DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pif1 and HELB

DIOPT Version :9

Sequence 1:	NP_001285587.1	Gene:	Pif1 / 33567	FlyBaseID:	FBgn0031540	Length:	663	Species:	Drosophila melanogaster
Sequence 2:	NP_001357214.1	Gene:	HELB / 92797	HGNCID:	17196	Length:	1087	Species:	Homo sapiens

Alignment Length:	649	Identity:	126/649 - (19%)
Similarity:	210/649 - (32%)	Gaps:	228/649 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly   120 TLQKKLREHLMSGKPSSFNDVSPVT------------TAEMILARKKAGLLSK------------ 160
            ||...:..|..|.......|:..||            .||..:|.....|:.|
Human   321 TLSNHMSFHAASESLKFLKDIGVVTYEKSCVFPYDLYHAERAIAFSICDLMKKPPWHLCVDVEKV 385

  Fly   161 -GSVTTPSPQGAKKRRFEELKEEKER--------GTMPAAKKLYASTTDE------SLRLSEEQM 210
             .|:.|..|:.:......|.|.::.|        .|......::.:..:|      .::|.::|:
Human   386 LASIHTTKPENSSDDALNESKPDEVRLENPVDVVDTQDNGDHIWTNGENEINAEISEVQLDQDQV 450

  Fly   211 EV---------LRACTSGKSVFFTGSAGTGKSFLLRRII-------------------------- 240
            ||         |....|......:|..|.||:.::.|:.
Human   451 EVPLDRDQVAALEMICSNPVTVISGKGGCGKTTIVSRLFKHIEQLEEREVKKACEDFEQDQNASE 515

  Fly   241 --------SALPPDGTV---ATASTGVAACLIGGTT-LHAF--AGIGGGDATMQRCLELASRPAN 291
                    |.|..|..:   .||.||.||.|:...| |||:  ..:.....:..:.:...::|
Human   516 EWITFTEQSQLEADKAIEVLLTAPTGKAAGLLRQKTGLHAYTLCQVNYSFYSWTQTMMTTNKP-- 578

  Fly   292 AQTWR--KCKRLIIDEISMVD-GQFFEKIEAVARHIRRNDRPFGGIQLILCGDFLQLPPVIKGDF 353
               |:  ..:.|::||.|:|. |.|...:..:..|.:.:       :||:.||..|||.:..|:.
Human   579 ---WKFSSVRVLVVDEGSLVSVGIFKSVLNLLCEHSKLS-------KLIILGDIRQLPSIEPGNL 633

  Fly   354 GAAPTATPQQRFCFQSSAWETCIQCVYELKQVHRQSD---------------PEFVKILNHLRIG 403
            ......|.:.|            .|..|||..||...               |:|...||
Human   634 LKDLFETLKSR------------NCAIELKTNHRAESQLIVDNATRISRRQFPKFDAELN----- 681

  Fly   404 HVNDSITSRLAATSKQKI-----EGNGILATQLCSHTNDANS--------INESKLENLDGDK-I 454
             ::|:.|..::...|..|     |.:   |:...|.||..:.        :.|:.|:|....: |
Human   682 -ISDNPTLPISIQDKTFIFVRLPEED---ASSQSSKTNHHSCLYSAVKTLLQENNLQNAKTSQFI 742

  Fly   455 LFKADDSD-----------ASMTRTLDQQIQAPSQLYLKVNAQVMLLKNINISNGLVNGARGVVV 508
            .|:..|.|           ..:|:  |.|    |:|...:..::...:|..:|:.|.....|...
Human   743 AFRRQDCDLINDCCCKHYTGHLTK--DHQ----SRLVFGIGDKICCTRNAYLSDLLPENISGSQQ 801

  Fly   509 RMEKDLPVVRF--------KNNQEY-----VCKHERWIIKTASGNHIT------RRQVP------ 548
            ..:.|.....|        ||.:::     :|..|.:.|.    |.:|      ||.:.
Human   802 NNDLDASSEDFSGTLPDFAKNKRDFESNVRLCNGEIFFIT----NDVTDVTFGKRRSLTINNMAG 862

  Fly   549 ---------------LKLAWAFSIHKSQGLTLDCVEMSLSKVFEAGQA--------YVALSRAK 589
                           :|.|||.:||..||.....|      |:..|:|        |.|::|.:
Human   863 LEVTVDFKKLMKYCRIKHAWARTIHTFQGSEEQTV------VYVVGKAGRQHWQHVYTAVTRGR 920

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pif1	NP_001285587.1	P-loop_NTPase	205..510	CDD:304359	80/396 (20%)
HELB	NP_001357214.1	recD_rel	<294..947	CDD:273632	126/649 (19%)
		DEXSc_RecD-like	457..654	CDD:350691	45/220 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	953..989
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1015..1051
		Nuclear export signal. /evidence=ECO:0000269\|PubMed:15146062	1055..1078
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0507
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.900