Protein Alignment Pif1 and PIF1

DIOPT Version :9

• Sequence 1: NP_001285587.1
• Gene: Pif1 / 33567
• FlyBaseID: FBgn0031540
• Length: 663
• Species: Drosophila melanogaster
• Sequence 2: NP_013650.1
• Gene: PIF1 / 854941
• SGDID: S000004526
• Length: 859
• Species: Saccharomyces cerevisiae
• Alignment Length: 710
• Identity: 211/710 - (29%)
• Similarity: 311/710 - (43%)
• Gaps: 219/710 - (30%)

  Fly   118 DATLQKK---------LREHLMSGKPSSF-----NDVSPVTTAEMI--LARKKAGLLSKGSVTTP 166
            |.:.|||         ||..|.  |.|||     |.:|...:.|||  ..:||.....|.:|.|.
Yeast   145 DQSSQKKSRSTGFKNPLRPALK--KESSFDELQNNSISQERSLEMINENEKKKMQFGEKIAVLTQ 207

  Fly   167 SPQGAKKRRFEELKEEKERGTMPAAKKLYASTTDESLR------------LSEEQMEVLRACTSG 219
            .|.      |.||:.:::               |.:|.            ||:||..:::...:|
Yeast   208 RPS------FTELQNDQD---------------DSNLNPHNGVKVKIPICLSKEQESIIKLAENG 251

  Fly   220 KSVFFTGSAGTGKSFLLRRIISALP----PDGTVATASTGVAACLIGGTTLHAFAGIGGGDATMQ 280
            .::|:|||||||||.|||.:|..|.    .:....|||||:|||.|||.|:|:|||||.|.....
Yeast   252 HNIFYTGSAGTGKSILLREMIKVLKGIYGRENVAVTASTGLAACNIGGITIHSFAGIGLGKGDAD 316

  Fly   281 RCLELASRP-ANAQTWRKCKRLIIDEISMVDGQFFEKIEAVARHIRRNDRPFGGIQLILCGDFLQ 344
            :..:...|. .:.:.|.....|::|||||:|.:..:|::.:||.||:|.:||||||||.||||.|
Yeast   317 KLYKKVRRSRKHLRRWENIGALVVDEISMLDAELLDKLDFIARKIRKNHQPFGGIQLIFCGDFFQ 381

  Fly   345 LPPVIKGDFGAAPTATPQQRFCFQSSAWETCIQCVYELKQVHRQ-SDPEFVKILNHLRIGHVNDS 408
            ||||.|..  ..||     :|.|:|.||:..::....|::|.|| .|.:|:.:||.:|:|:::|.
Yeast   382 LPPVSKDP--NRPT-----KFAFESKAWKEGVKMTIMLQKVFRQRGDVKFIDMLNRMRLGNIDDE 439

  Fly   409 ITSRLAATSKQKIEGNGILATQLCSHTNDANSINESKLENLDGDKILFKADD----SDASMTRTL 469
             |.|......:.:..:.|:..:|.|...:....|.|:|..|.|...:|.|.|    .|..:...|
Yeast   440 -TEREFKKLSRPLPDDEIIPAELYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALEDEELKERL 503

  Fly   470 DQQIQAPSQLYLKVNAQVMLLKNINISNGLVNGARGVVVR------------------------- 509
            .|...||.:|:|||.||||::||::.:  ||||:.|.|:.
Yeast   504 LQNFLAPKELHLKVGAQVMMVKNLDAT--LVNGSLGKVIEFMDPETYFCYEALTNDPSMPPEKLE 566

  Fly   510 ------------MEKD------------------------------------------------- 513
                        ||::
Yeast   567 TWAENPSKLKAAMEREQSDGEESAVASRKSSVKEGFAKSDIGEPVSPLDSSVFDFMKRVKTDDEV 631

  Fly   514 ------------------------LPVVRFK----NNQEYVCKHERWIIKTASGNH-ITRRQVPL 549
                                    ||:||||    :.:..:.:.|.|.|:..:... ::|.|:||
Yeast   632 VLENIKRKEQLMQTIHQNSAGKRRLPLVRFKASDMSTRMVLVEPEDWAIEDENEKPLVSRVQLPL 696

  Fly   550 KLAWAFSIHKSQGLTLDCVEMSLSKVFEAGQAYVALSRAKSLQSIRILDFDAKQVWANPHVLQFY 614
            .|||:.|||||||.||..|::.|.:|||.||||||||||.|.:.:::|:||..::.|:..|:.||
Yeast   697 MLAWSLSIHKSQGQTLPKVKVDLRRVFEKGQAYVALSRAVSREGLQVLNFDRTRIKAHQKVIDFY 761

  Fly   615 ----------------KGFRRKLMDTTMIPLGPKNKDKKPGDKAANSALAKLKKSLINKP 658
                            :..:::.:|...   |||.|.|              .||..|.|
Yeast   762 LTLSSAESAYKQLEADEQVKKRKLDYAP---GPKYKAK--------------SKSKSNSP 804

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pif1	NP_001285587.1	P-loop_NTPase	205..510	CDD:304359	124/351 (35%)
PIF1	NP_013650.1	RecD	4..763	CDD:223581	201/650 (31%)
		DEAD-like_helicase_N	237..542	CDD:421690	124/314 (39%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346091
Domainoid	1	1.000	170	1.000	Domainoid score	I774
eggNOG	1	0.900	-	-		E1_COG0507
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	287	1.000	Inparanoid score	I539
Isobase	1	0.950	-	0	Normalized mean entropy	S2212
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002406
OrthoInspector	1	1.000	-	-		otm46814
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR23274
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R921
SonicParanoid	1	1.000	-	-		X3535
TreeFam	0	0.000	Not matched by this tool.
	12	11.870