DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pif1 and Helb

DIOPT Version :9

Sequence 1:	NP_001285587.1	Gene:	Pif1 / 33567	FlyBaseID:	FBgn0031540	Length:	663	Species:	Drosophila melanogaster
Sequence 2:	NP_536694.2	Gene:	Helb / 117599	MGIID:	2152895	Length:	1074	Species:	Mus musculus

Alignment Length:	756	Identity:	151/756 - (19%)
Similarity:	253/756 - (33%)	Gaps:	254/756 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 NDAVLTCAVNMEWTNAVGITGRKLAYKTATLRLVR-----------------------------N 39
            :|.||.|..::..|....:..|::.|:  .::|||                             :
Mouse   226 SDWVLGCIEDVLGTQPWKLGFRRITYR--EMKLVRCEASWTAFSQCPSLLQLMTPLQKNALVIYS 288

  Fly    40 DLRE-------LFVEVT------------PEKLKPLKFKLKDL--MVHKK---FMSEGKATFNFK 80
            .||:       .::||.            .|..:.|.| |||:  ::::|   |:||     .::
Mouse   289 KLRQTCREDGHTYIEVKDLTSGLSEHMSFEEACQSLAF-LKDIDVVIYEKDYVFLSE-----LYE 347

  Fly    81 AEK------CTLYLSNAPPGTLMFFLRTIFVKMNG-----------------NEGGSQPDDATLQ 122
            ||:      |.  |.:.||..|...::.:...:.|                 .|.||:..|:.|.
Mouse   348 AEQDIASSICE--LMSRPPWHLKVDVKNVLASIRGAKPNDPGSAEAVEGSKPEEVGSEQGDSVLD 410

  Fly   123 --------KKLREHLMSGKPSSFNDV----SPVTTAEMILARKKAGLLSKGSV--TTPSPQGAKK 173
                    :...||:.:   :..|||    ..|...|.|.|.....|..||..  ||     ...
Mouse   411 AQDGDDHVRSNGEHVAN---AEINDVPLDQDQVVALETICANAVTVLSGKGGCGKTT-----IVS 467

  Fly   174 RRFEELKEEKERGTMPAAKKLYASTTDESLRLSEEQMEVLRACTSG--KSVFFTGSAGTGKSFLL 236
            |.|:.::..:|.....|.:..     ::....|||.::..:...:|  |:|....:|.|||:..|
Mouse   468 RLFKHMEHLEETEVQQACEDF-----EQDQEASEEWLDCPKQSPAGVDKAVEVLLTAPTGKAAGL 527

  Fly   237 RRIISALPPDGTVATASTGVAACLIGGTTLHAFAGIGGGDATMQRCLELASRPANAQTWRKCKRL 301
            .|..:.||          ....|.:.    ::|        .|.:......:|....|.|.   |
Mouse   528 LRQRTDLP----------AYTLCQVN----YSF--------YMWKTKNEVDKPWKFSTVRV---L 567

  Fly   302 IIDEISMVD-GQFFEKIEAVARHIRRNDRPFGGIQLILCGDFLQLPPVIKGDFGAAPTATPQQRF 365
            ::||.|:|. |.|...::.:.:|.:.:       :||:.||..|||.:..|:.......|.:.|
Mouse   568 VVDEGSLVSVGIFKSVLQLLCKHSKLS-------KLIILGDVRQLPSIEPGNMLQDVFETLKSR- 624

  Fly   366 CFQSSAWETCIQCVYELKQVHRQSD---------------PEFVKILN----------------- 398
                       ||..|||..||...               |:|...||
Mouse   625 -----------QCAIELKTNHRTESQLIVDNATRISRRQFPKFDAELNICGNPTLPLSIQDKTFI 678

  Fly   399 HLRIGHVNDSIT--------SRLAATSKQKIEGNGILATQ---------------------LCSH 434
            .:|:.. .||.:        |.|....|..::|....:.:                     .|.|
Mouse   679 FVRLPE-EDSRSQSSKGEHRSNLYTAVKTLLQGKDFCSFESSKTSQFIAFRRQDCDLINDCCCKH 742

  Fly   435 -TNDANSINESKLENLDGDKILFKADDSDASMTRTLDQQIQ------APSQLYLKVNAQVMLLKN 492
             |......:|.||....||||....:...:.:....||:.:      ||..       ...:.::
Mouse   743 YTGHLIKDHEKKLIFAVGDKICCTRNAYLSDLLPDKDQEAEGKGYGDAPDD-------DAKIKQD 800

  Fly   493 INISNGLVNGARGVVVRMEKDLPVVRFKNNQEYVCKHERWIIKTASGNHITRRQVPLKLAWAFSI 557
            ...|..|.||.   :..:.:|:..|.||..:.....:|..:..|...:.: .....:|.|||.:|
Mouse   801 FESSTRLCNGE---IFFITRDVTDVTFKRKRLLTINNEAGLEVTVDFSKL-MANCQIKHAWARTI 861

  Fly   558 HKSQGLTLDCVEMSLSKVFEAGQA--------YVALSRAKS 590
            |..||...:.|      |:..|:|        |.|::|.:|
Mouse   862 HTFQGSEENTV------VYVVGKAGRQHWQHVYTAVTRGRS 896

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pif1	NP_001285587.1	P-loop_NTPase	205..510	CDD:304359	74/375 (20%)
Helb	NP_536694.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..38
		recD_rel	<273..922	CDD:273632	141/707 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	380..420		6/39 (15%)
		P-loop_NTPase	435..635	CDD:304359	56/253 (22%)
		UvrD_C_2	855..901	CDD:304668	15/48 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	932..1014
		Nuclear export signal. /evidence=ECO:0000269\|PubMed:26774285	1022..1046
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1040..1074
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0507
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900