Protein Alignment AGO3 and PIWIL1

DIOPT Version :10

• Sequence 1: NP_001036627.2
• Gene: AGO3 / 3355150
• FlyBaseID: FBgn0250816
• Length: 867
• Species: Drosophila melanogaster
• Sequence 2: NP_004755.2
• Gene: PIWIL1 / 9271
• HGNCID: 9007
• Length: 861
• Species: Homo sapiens
• Alignment Length: 883
• Identity: 332/883 - (37%)
• Similarity: 501/883 - (56%)
• Gaps: 87/883 - (9%)

  Fly     3 GRGNLLSLFNKNAGNMGKSISSKDHEIDSGLDFNNSESSGERLLSSHNIETDLITTLQHVNISVG 67
            |||       :..|..|.:..|:..:|.:|....:....|.|....|::..:....|.||
Human    48 GRG-------RQRGTAGGTAKSQGLQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHV----- 100

  Fly    68 RGRARLIDTLKTDDHTSNQFITSESKENITKKTKGPESEAIASENGLFFPDLIYGSKGSSVNIYC 132
                                     ||:.|                        ||.|..|.:..
Human   101 -------------------------KESKT------------------------GSSGIIVRLST 116

  Fly   133 NYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPILLPNKMTVF 197
            |:.:||:.....::.|.:.:.|.:::..||...|..|:|.:|....|||..|:||..|..|:|..
Human   117 NHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGKCHAFDGTILFLPKRLQQKVTEV 181

  Fly   198 ISK---AEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKL 259
            .||   .|||.:.|.:..:.......|.|.|||:|.|::|::|..:..|..::|:.|..||..:|
Human   182 FSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRL 246

  Fly   260 EVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEH-YQESARKMLVGNIVLTR 323
            .:|||:.|::.:|:..:|||.||||::|..:|||:.:.:.|.|..|| :||...|.|:|.:|||:
Human   247 VIWPGFTTSILQYENSIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTK 311

  Fly   324 YNNRTYKINDICFDQNPTCQFEIKTGC-TSYVEYYKQYHNINIKDVNQPLIYSIKKSRGIPAERE 387
            |||:||:::||.:||||...|:...|. .|::|||::.:|..|.|:.||::.|..|.|..|....
Human   312 YNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTL 376

  Fly   388 NLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSW 452
            .....|||||||||||.|::|:|..:|:::|..||::|.|||..:.:..:.:......|..|..|
Human   377 PGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDW 441

  Fly   453 GLSLTNNSNKISGRQMDIEQIYFSKISVSAGRS-------AEFSKHAVTNEMLKVVHLSKWIIIH 510
            |||..:|....|||.:..|:|:      ..|::       |::||......::.|..|..|::|:
Human   442 GLSFDSNLLSFSGRILQTEKIH------QGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIY 500

  Fly   511 LRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRR 575
            .|...:||.||:.|:.:...::||.:....||.:| ||.:||::.|::.:|.:||:|||:..:.|
Human   501 TRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEVD-DRTEAYLRVLQQKVTADTQIVVCLLSSNR 564

  Fly   576 DDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMIC 640
            .|:|.||||..|::.|.|||.:.|:||.....:.::..||.||||||:||.||.|.||.|.|||.
Human   565 KDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIV 629

  Fly   641 GIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLP 705
            |||.|||.:....|:|.||||||...::|:|:.:.|.:.:|:|:||....:.||:.:...|..:|
Human   630 GIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMP 694

  Fly   706 TNIIIYRDGIGDGQLYTCLNYEIPQFEMVC------GNRIKISYIVVQKRINTRIFSGSGIHLEN 764
            :.||:||||:|||||.|.:|||:||| :.|      |...:::.|||:||:|||.|:.||..|:|
Human   695 SRIIVYRDGVGDGQLKTLVNYEVPQF-LDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQN 758

  Fly   765 PLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWA 829
            ||||||:|..:|:...||||:|||.||.|:|:||||.|:.|:....||.||:|:||||.:||||.
Human   759 PLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWP 823

  Fly   830 GTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL 867
            |.:|:||.|.||||||:|:||||.|:...:||.:|:||
Human   824 GVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL 861

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	59/135 (44%)
		Piwi_piwi-like_Euk	412..850	CDD:240016	192/450 (43%)
PIWIL1	NP_004755.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..64		6/22 (27%)
		GAGE	16..106	CDD:461753	17/118 (14%)
		D-box. /evidence=ECO:0000269|PubMed:28552346	217..224		2/6 (33%)
		PAZ	277..415	CDD:198017	59/137 (43%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000269|PubMed:21193640	316..318		1/1 (100%)
		Piwi_piwi-like_Euk	401..844	CDD:240016	192/450 (43%)
		MID region. /evidence=ECO:0000250|UniProtKB:Q9JMB7	479..615		52/136 (38%)