DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment AGO3 and Piwil4

DIOPT Version :9

Sequence 1:	NP_001036627.2	Gene:	AGO3 / 3355150	FlyBaseID:	FBgn0250816	Length:	867	Species:	Drosophila melanogaster
Sequence 2:	XP_008764202.1	Gene:	Piwil4 / 689972	RGDID:	1311015	Length:	848	Species:	Rattus norvegicus

Alignment Length:	881	Identity:	298/881 - (33%)
Similarity:	476/881 - (54%)	Gaps:	47/881 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSGRGNLLSLFNKNAGNMGKSISSKDHEIDSGLDFNNSESSGERLLSSHNIETDLITTLQHVNIS 65
            ||||..:.:. ....|:..:.:.....::     ...|.|.|:...||   .|.:|:....:.:|
  Rat     1 MSGRARVRAR-GITTGHSAREVERAPRDL-----MVTSVSPGDNEASS---RTSVISQPHELGVS 56

  Fly    66 VGRGRARLIDTLKT--DDHTSNQFITSESKENITKKTKGPESEAIASENGLFFPDLIYGSKGSSV 128
            .|.||..|::....  .|.......|.|      |.|...:|:.              ||.|..|
  Rat    57 SGDGRRTLVEKRGNGRQDFVDLGVCTRE------KLTHVKDSKT--------------GSSGIPV 101

  Fly   129 NIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPILLPNK 193
            .:..|...|...:...::.|.|.:.|.:.|..||...|.:|.......|.|||.:|:|...|.:|
  Rat   102 QLATNLFHLDLPQDWQLYQYHVTYSPDLASRRLRTALLYNHSILSDKAKAFDGTSLFLSEKLDHK 166

  Fly   194 MTVFIS---KAEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIP 255
            :|...|   :.|.|::.:.:..:.......|.|.:|::|.::.|.|:..:..|..:.||.|..||
  Rat   167 VTELTSETQRGETVKITLTLTSELFPNSPMCIQFFNVIFRKIFKKLSMYQIGRNYYKPSEPVEIP 231

  Fly   256 LAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDL-YQQNVEHYQESARKMLVGNI 319
            ..||.:|||:..:|..::..|:...||::::|..:||||.:.|| .:.::..:.|:..|.|||.|
  Rat   232 QYKLSLWPGFAISVSRFESKLLFNADVNYKVLRNETVLEFMTDLCLRTDMSCFTETCHKQLVGLI 296

  Fly   320 VLTRYNNRTYKINDICFDQNPTCQFEIKTGC-TSYVEYYKQYHNINIKDVNQPLIYS-IKKSRGI 382
            |||||||:||:|:||.:...||..|:.:.|. |:||:||||.::|.:.|:|||::.| :|:.|..
  Rat   297 VLTRYNNKTYRIDDIDWSVKPTHAFQKRDGSETTYVDYYKQQYDITLSDLNQPVLVSLLKRRRND 361

  Fly   383 PAERENLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQE 447
            .:|.:.:.  ||||||:||||..:..||.:||:.:|..||:||..||..|.:..:::......:.
  Rat   362 NSEPQMVH--LIPELCFLTGLTSQATSDFRLMKAVAEETRLSPMGRQQQLARLADDIQRNQVTRF 424

  Fly   448 ILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLR 512
            .|.:|||.. .:...::||.:..|:|.....:.....:|::||...:.::|....|:.|:|:...
  Rat   425 ELETWGLHF-GSQLSLTGRVVPSEKILLQDHTCQPAFAADWSKDMRSCKVLSSEPLNTWLIVCCS 488

  Fly   513 NYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDD 577
            .......|.|..:::...|.|.::.:|.:|.:|.:.. |:::|::.::..:.|:|:||..:.:.:
  Rat   489 RAEHLIESFLSCLRRVGGSTGFSVGHPKIIKVDENPA-AFLRAIQLHVNPDVQLVMCILPSNQKN 552

  Fly   578 RYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGI 642
            .|.:||:...|:.|:|||.:..:||.....:.||..||.:||.|||||.||.|:||.|::|:.||
  Rat   553 YYDSIKRYLSSDCPVPSQCVLTRTLNKQGMMLSVATKIAMQMTCKLGGELWAVEIPLKSLMVVGI 617

  Fly   643 DSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTN 707
            |...|..::..:|..|||||||..::|:|:.|:|....:|.:.|......||..:.|.|..||..
  Rat   618 DICRDALSKDVAVVGFVASINSRITRWFSRCVLQRTAADIADCLKVCMTGALNKWYKHNHDLPAR 682

  Fly   708 IIIYRDGIGDGQLYTCLNYEIPQF---EMVCGN---RIKISYIVVQKRINTRIFSGSGIHLENPL 766
            ||.||||:|:|||...|.||:||.   ...||:   ..::|.:||:||...|:|:.||..|:||.
  Rat   683 IIAYRDGVGNGQLKAVLEYEVPQLLSSVTECGSDARSCRLSVVVVRKRCLLRLFAESGHTLQNPP 747

  Fly   767 PGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGT 831
            .|||||...|:...|||:|.||...:|||:||:|.|:.||....||.:|:|::|||.|||||.|.
  Rat   748 LGTVVDSEATRPEWYDFYLTSQTANRGTVSPTYYNVIYDDNALKPDHMQRLTFKLCHLYYNWQGV 812

  Fly   832 VRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL 867
            :.:||.|.|||||.:|:.|||.::.:..|:.|||||
  Rat   813 ISVPAPCQYAHKLTFLVAQSIHKEPSLELANKLFYL 848

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	61/136 (45%)
AGO3	NP_001036627.2	Piwi_piwi-like_Euk	412..850	CDD:240016	162/443 (37%)
Piwil4	XP_008764202.1	ArgoN	95..205	CDD:293095	29/109 (27%)
		PAZ_piwi_like	265..381	CDD:239211	53/117 (45%)
		Piwi_piwi-like_Euk	389..831	CDD:240016	162/443 (37%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166340831
Domainoid	1	1.000	310	1.000	Domainoid score	I1281
eggNOG	1	0.900	-	-		E1_KOG1042
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	615	1.000	Inparanoid score	I865
OMA	1	1.010	-	-		QHG54391
OrthoDB	1	1.010	-	-		D220258at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm44598
orthoMCL	1	0.900	-	-		OOG6_100694
Panther	1	1.100	-	-	O	PTHR22891
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X548
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	12	11.810