DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment AGO3 and Piwil2

DIOPT Version :9

Sequence 1:	NP_001036627.2	Gene:	AGO3 / 3355150	FlyBaseID:	FBgn0250816	Length:	867	Species:	Drosophila melanogaster
Sequence 2:	NP_001351250.1	Gene:	Piwil2 / 57746	MGIID:	1930036	Length:	1042	Species:	Mus musculus

Alignment Length:	903	Identity:	321/903 - (35%)
Similarity:	491/903 - (54%)	Gaps:	68/903 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 GRGNLLSLFNKNAGNMGKSISSKDHEIDSGLDFNNSESS--GERLLSSHNIETDLITTLQHVNIS 65
            |||...::..|:......|:  .|..:.:..|...:|:|  ..|:|...:.|..|: .|.....|
Mouse   170 GRGLSANMVRKDREEPRSSL--PDPSVLAAGDSKLAEASVGWSRMLGRGSSEVSLL-PLGRAASS 231

  Fly    66 VGRG----------RARLIDTL-------KTDDHTSN--QFITSESKENITKKTKGPESEAIASE 111
            :|||          .||....|       .|..|:::  ..:|.|.|          |.|.:..:
Mouse   232 IGRGMDKPPSAFGLTARDPPRLPQPPALSPTSLHSADPPPVLTMERK----------EKELLVKQ 286

  Fly   112 NGLFFPDLIYGSKGSSVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGT 176
                      ||||:..::..|.:|:.. .::.|:.|.|.|.|.::...:|...|.||:...|..
Mouse   287 ----------GSKGTPQSLGLNLIKIQC-HNEAVYQYHVTFSPSVECKSMRFGMLKDHQSVTGNV 340

  Fly   177 KTFDGNTLYLPILLPNKMTVFIS-KAEDVELQIRILYKKKEEMRN--CTQLYNILFDRVMKVLNY 238
            ..|||:.||||:.|...:.:... |.:|.|:.|:|...|..|..:  |...||::|.||||:|:.
Mouse   341 TAFDGSILYLPVKLQQVVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDM 405

  Fly   239 VKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQN 303
            ....|..:||:...::...:|::||||..::....|||.|..||||:::...:||:::..:||||
Mouse   406 KLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVMHAIYQQN 470

  Fly   304 VEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGC-TSYVEYYKQYHNINIKD 367
            .||:|:...|:|||:||:||||||||:|:|:.:::.|...|.:..|. .:::|||.:.:.|.:|:
Mouse   471 KEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKE 535

  Fly   368 VNQPLIYSIKKSRGIPAERENL-------QFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSP 425
            .:|||:  |.:    |:||:|.       :..|:|||.::||:.::::.|.:.|:::.....:||
Mouse   536 DDQPLL--IHR----PSERQNNHGMLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLTQQINLSP 594

  Fly   426 NQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSK 490
            .|...||....:.:|....|...|..|||||..:.:||.||.:.:|:|.....|........:.|
Mouse   595 KQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLLPMERINLRNTSFVTSEDLNWVK 659

  Fly   491 HAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQA 555
            ....:..:..:.:..|.:.:.:.....|..|::.:::....:||.||.|..:.|..|||:.||:.
Mouse   660 EVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIRT 724

  Fly   556 LRR--NITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQ 618
            ::.  .:....||||||....|||.|.||||:||.:.|:||||||.:|:....::|||.|||:||
Mouse   725 IQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQ 789

  Fly   619 MNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIV 683
            |||||||.||.|.||.|.:|:.|:|.|||||....||..||||||.:.::|||:.|.|...:|||
Mouse   790 MNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIV 854

  Fly   684 NGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQ----FEMVCGNRIKISYI 744
            :.|......:||.|.:.|..||..|::||||:.||||.|..||||||    ||.......|:...
Mouse   855 DSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDNYHPKMVVF 919

  Fly   745 VVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNY 809
            ||||:|:|.::..:..|...|.||||||..||.....||:|::..||||...||||:.:.:..|.
Mouse   920 VVQKKISTNLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANL 984

  Fly   810 GPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL 867
            .||.:|:|::|||.:|:||.||:|:||.|.||||||:|.||.:..:.|..|...||:|
Mouse   985 SPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCGNLFFL 1042

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	49/141 (35%)
AGO3	NP_001036627.2	Piwi_piwi-like_Euk	412..850	CDD:240016	182/443 (41%)
Piwil2	NP_001351250.1	Atrophin-1	<73..271	CDD:331285	23/103 (22%)
		ArgoL1	408..455	CDD:312283	16/46 (35%)
		PAZ_piwi_like	456..573	CDD:239211	47/122 (39%)
		Piwi_piwi-like_Euk	581..1025	CDD:240016	182/443 (41%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167837114
Domainoid	1	1.000	310	1.000	Domainoid score	I1322
eggNOG	1	0.900	-	-		E1_KOG1042
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H23071
Inparanoid	1	1.050	615	1.000	Inparanoid score	I894
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54391
OrthoDB	1	1.010	-	-		D220258at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm42534
orthoMCL	1	0.900	-	-		OOG6_100694
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3665
SonicParanoid	1	1.000	-	-		X548
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.700