Protein Alignment AGO3 and piwil1

DIOPT Version :10

• Sequence 1: NP_001036627.2
• Gene: AGO3 / 3355150
• FlyBaseID: FBgn0250816
• Length: 867
• Species: Drosophila melanogaster
• Sequence 2: NP_899181.1
• Gene: piwil1 / 368200
• ZFINID: ZDB-GENE-030813-2
• Length: 858
• Species: Danio rerio
• Alignment Length: 826
• Identity: 311/826 - (37%)
• Similarity: 471/826 - (57%)
• Gaps: 55/826 - (6%)

  Fly    60 QHVNISVGRGRARLIDTLKTDDHTSNQFITSESKENITKKTKGPESEAIASENGLFFPDLIYGSK 124
            |.|.|....||.|       |.|.|... |.:..|:: |::|                   .|..
Zfish    70 QQVKIGERGGRRR-------DFHDSGVH-TRQLMEHV-KESK-------------------TGVS 106

  Fly   125 GSSVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPIL 189
            |:::.:..|:::|.:.....::.|.|.:.||::|..||...|..|::.||...||||..|:||..
Zfish   107 GTAIELRANFMRLLSRPMWALYQYHVDYKPPMESRRLRSALLFQHEETLGKAHTFDGAILFLPNK 171

  Fly   190 LPNKMTVFISK---AEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRP 251
            |.|..||..|:   .|.||:.:.:..:.......|.|.|||||.|::::||..:..|..::|..|
Zfish   172 LRNAETVLCSETRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDDP 236

  Fly   252 KIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQ-NVEHYQESARKML 315
            ..||..:|.:|||::|.:.:|:..:|||.||||::|..:|||:.:..|.|| ..:.:.|:..|.|
Zfish   237 FNIPQHRLTIWPGFMTTILQYESSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQRFPEACTKEL 301

  Fly   316 VGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCT--SYVEYYKQYHNINIKDVNQPLIYSIKK 378
            ||.|:||:|||:||:|:||.:|..|...|  |.|.|  |:..|:|..:.::|.|.||.|:.|..|
Zfish   302 VGLIILTKYNNKTYRIDDIAWDHTPNNTF--KKGDTEISFKNYFKSQYGLDITDGNQVLLVSHVK 364

  Fly   379 SRGIPAERENLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTP 443
            ..|...........|:||.||||||.|::|:|..:|:::|:.||:||.||:..:|:...|::...
Zfish   365 RLGPSGRPPPGPAMLVPEFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNG 429

  Fly   444 AAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGR-------SAEFSKHAVTNEMLKVV 501
            ..|..|.:||||..|....::||.:..|:|      :..||       :|::||......::..:
Zfish   430 DVQNELTTWGLSFENKLLSLNGRVLPSERI------IQGGRAFEYNPWTADWSKEMRGLPLISCM 488

  Fly   502 HLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQM 566
            .|..|::.:.|.....|.|||..:.:....:|:.:....||..: ||.::.::||::|:...|||
Zfish   489 SLDNWLMFYTRRNADVAQSLLQTLNKVSGPMGIRMQRAVMIEYE-DRQESLLRALQQNVARETQM 552

  Fly   567 VVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVK 631
            ||.|....|.|:|..:||..|.:.|.|||.:.::|:.....:.:|..||.||||||:||.||:|:
Zfish   553 VVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVE 617

  Fly   632 IPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKM 696
            ||.:.:||.|||.|||.:....|:.|.|||:|...|:|:||.|:|.:.:||::.|..|.:.|||.
Zfish   618 IPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKA 682

  Fly   697 YRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQF-----EMVCGNRIKISYIVVQKRINTRIFS 756
            |.|.|..||:.||:||||:|||.|.:.::||:||.     .|......|:|.:||:|||::|.|:
Zfish   683 YLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFA 747

  Fly   757 GSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKL 821
            .....:.||.||||:|..:|:...||||:|||.||.|.|.||||.|:.|:....||.:|:|:|||
Zfish   748 RIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDNSGLKPDHMQRLTYKL 812

  Fly   822 CFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL 867
            |.:||||.|.||:||.|.||||||:|:||||.::....|.:.|:||
Zfish   813 CHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHKEPNMNLDDFLYYL 858

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	55/136 (40%)
		Piwi_piwi-like_Euk	412..850	CDD:240016	180/449 (40%)
piwil1	NP_899181.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..56
		GAGE	17..104	CDD:461753	13/61 (21%)
		PAZ	275..412	CDD:198017	56/138 (41%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000250|UniProtKB:Q9JMB7	314..316		1/1 (100%)
		Piwi_piwi-like_Euk	398..841	CDD:240016	180/449 (40%)
		MID region. /evidence=ECO:0000250|UniProtKB:Q9JMB7	476..612		44/136 (32%)