DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment AGO3 and piwil1

DIOPT Version :9

Sequence 1:	NP_001036627.2	Gene:	AGO3 / 3355150	FlyBaseID:	FBgn0250816	Length:	867	Species:	Drosophila melanogaster
Sequence 2:	NP_899181.1	Gene:	piwil1 / 368200	ZFINID:	ZDB-GENE-030813-2	Length:	858	Species:	Danio rerio

Alignment Length:	826	Identity:	311/826 - (37%)
Similarity:	471/826 - (57%)	Gaps:	55/826 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly    60 QHVNISVGRGRARLIDTLKTDDHTSNQFITSESKENITKKTKGPESEAIASENGLFFPDLIYGSK 124
            |.|.|....||.|       |.|.|... |.:..|:: |::|                   .|..
Zfish    70 QQVKIGERGGRRR-------DFHDSGVH-TRQLMEHV-KESK-------------------TGVS 106

  Fly   125 GSSVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPIL 189
            |:::.:..|:::|.:.....::.|.|.:.||::|..||...|..|::.||...||||..|:||..
Zfish   107 GTAIELRANFMRLLSRPMWALYQYHVDYKPPMESRRLRSALLFQHEETLGKAHTFDGAILFLPNK 171

  Fly   190 LPNKMTVFISK---AEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRP 251
            |.|..||..|:   .|.||:.:.:..:.......|.|.|||||.|::::||..:..|..::|..|
Zfish   172 LRNAETVLCSETRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDDP 236

  Fly   252 KIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQ-NVEHYQESARKML 315
            ..||..:|.:|||::|.:.:|:..:|||.||||::|..:|||:.:..|.|| ..:.:.|:..|.|
Zfish   237 FNIPQHRLTIWPGFMTTILQYESSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQRFPEACTKEL 301

  Fly   316 VGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCT--SYVEYYKQYHNINIKDVNQPLIYSIKK 378
            ||.|:||:|||:||:|:||.:|..|...|  |.|.|  |:..|:|..:.::|.|.||.|:.|..|
Zfish   302 VGLIILTKYNNKTYRIDDIAWDHTPNNTF--KKGDTEISFKNYFKSQYGLDITDGNQVLLVSHVK 364

  Fly   379 SRGIPAERENLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTP 443
            ..|...........|:||.||||||.|::|:|..:|:::|:.||:||.||:..:|:...|::...
Zfish   365 RLGPSGRPPPGPAMLVPEFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNG 429

  Fly   444 AAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGR-------SAEFSKHAVTNEMLKVV 501
            ..|..|.:||||..|....::||.:..|:|      :..||       :|::||......::..:
Zfish   430 DVQNELTTWGLSFENKLLSLNGRVLPSERI------IQGGRAFEYNPWTADWSKEMRGLPLISCM 488

  Fly   502 HLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQM 566
            .|..|::.:.|.....|.|||..:.:....:|:.:....||..: ||.::.::||::|:...|||
Zfish   489 SLDNWLMFYTRRNADVAQSLLQTLNKVSGPMGIRMQRAVMIEYE-DRQESLLRALQQNVARETQM 552

  Fly   567 VVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVK 631
            ||.|....|.|:|..:||..|.:.|.|||.:.::|:.....:.:|..||.||||||:||.||:|:
Zfish   553 VVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVE 617

  Fly   632 IPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKM 696
            ||.:.:||.|||.|||.:....|:.|.|||:|...|:|:||.|:|.:.:||::.|..|.:.|||.
Zfish   618 IPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKA 682

  Fly   697 YRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQF-----EMVCGNRIKISYIVVQKRINTRIFS 756
            |.|.|..||:.||:||||:|||.|.:.::||:||.     .|......|:|.:||:|||::|.|:
Zfish   683 YLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFA 747

  Fly   757 GSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKL 821
            .....:.||.||||:|..:|:...||||:|||.||.|.|.||||.|:.|:....||.:|:|:|||
Zfish   748 RIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDNSGLKPDHMQRLTYKL 812

  Fly   822 CFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL 867
            |.:||||.|.||:||.|.||||||:|:||||.::....|.:.|:||
Zfish   813 CHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHKEPNMNLDDFLYYL 858

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	55/136 (40%)
AGO3	NP_001036627.2	Piwi_piwi-like_Euk	412..850	CDD:240016	180/449 (40%)
piwil1	NP_899181.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..56
		GAGE	17..104	CDD:283487	13/61 (21%)
		ArgoN	104..203	CDD:293095	30/98 (31%)
		PAZ	275..412	CDD:198017	56/138 (41%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000250\|UniProtKB:Q9JMB7	314..316		1/1 (100%)
		Piwi_piwi-like_Euk	398..841	CDD:240016	180/449 (40%)
		MID region. /evidence=ECO:0000250\|UniProtKB:Q9JMB7	476..612		44/136 (32%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580667
Domainoid	1	1.000	92	1.000	Domainoid score	I7636
eggNOG	1	0.900	-	-		E1_KOG1042
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	568	1.000	Inparanoid score	I1032
OMA	1	1.010	-	-		QHG54391
OrthoDB	1	1.010	-	-		D220258at2759
OrthoFinder	1	1.000	-	-		FOG0003636
OrthoInspector	1	1.000	-	-		otm24422
orthoMCL	1	0.900	-	-		OOG6_100694
Panther	1	1.100	-	-	O	PTHR22891
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3665
SonicParanoid	1	1.000	-	-		X548
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	14	13.840