DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment AGO3 and Piwil2

DIOPT Version :9

Sequence 1:	NP_001036627.2	Gene:	AGO3 / 3355150	FlyBaseID:	FBgn0250816	Length:	867	Species:	Drosophila melanogaster
Sequence 2:	XP_006252333.2	Gene:	Piwil2 / 306011	RGDID:	1306964	Length:	1005	Species:	Rattus norvegicus

Alignment Length:	903	Identity:	322/903 - (35%)
Similarity:	492/903 - (54%)	Gaps:	68/903 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 GRGNLLSLFNKNAGNMGKSISSKDHEIDSGLDFNNSESS--GERLLSSHNIETDLITTLQHVNIS 65
            |||...::..|:......|:  .|..:.:..|...:|:|  ..|:|...:.:..|: .|.....|
  Rat   133 GRGLSANVVRKDREESRSSL--PDPSVLAAGDSKLAEASVGWSRMLGRGSSDVSLL-PLGRAAGS 194

  Fly    66 VGRG----------------RARLIDTLK-TDDHTSN--QFITSESKENITKKTKGPESEAIASE 111
            :|||                |.....||. |..|:|:  ..:|.|.|          |.|.:..:
  Rat   195 IGRGVDKPPSAFGLTARDPPRLPQPPTLSPTSLHSSDPPPVLTIERK----------EKELLVKQ 249

  Fly   112 NGLFFPDLIYGSKGSSVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGT 176
                      ||||:..::..|.:|:.. .::.|:.|.|.|.|.::...:|...|.||:...|..
  Rat   250 ----------GSKGTPQSLGLNLIKIQC-HNEAVYQYHVTFSPNVECKSMRFGMLKDHQSVTGNV 303

  Fly   177 KTFDGNTLYLPILLPNKMTVFIS-KAEDVELQIRILYKKKEEMRN--CTQLYNILFDRVMKVLNY 238
            ..|||:.||||:.|...:.:... |.:|.|:.|:|...|..|..:  |...||::|.||||:|:.
  Rat   304 TAFDGSILYLPVKLQQLVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDM 368

  Fly   239 VKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQN 303
            ....|..:||:...::...:|::||||..::....|||.|..||||:::...:||:::..:||||
  Rat   369 KLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVMHAIYQQN 433

  Fly   304 VEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGC-TSYVEYYKQYHNINIKD 367
            .||:|:...|:|||:||:||||||||:|:|:.:::.|...|.:..|. .:::|||.:.:.|.:|:
  Rat   434 KEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKE 498

  Fly   368 VNQPLIYSIKKSRGIPAERENL-------QFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSP 425
            .:|||:  |.:    |:||:|.       :..|:|||.::||:.::::.|.:.|:::.....:||
  Rat   499 DDQPLL--IHR----PSERQNNHGTLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLTQQINLSP 557

  Fly   426 NQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSK 490
            .|...||....:.:|....|...|..|||||..:.:||.||.:.:|:|.....|........:.|
  Rat   558 KQHHSALECLLQRISQNETANNELTRWGLSLQKDVHKIEGRLLPMERINLRNTSFVTSEDLNWVK 622

  Fly   491 HAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQA 555
            ....:..:..:.:..|.:.:.:.....|..|::.:::....:||.||.|..:.|..|||:.||:.
  Rat   623 EVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIRT 687

  Fly   556 LRR--NITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQ 618
            ::.  .:....||||||....|||.|.||||:||.:.|:||||||.:|:....::|||.|||:||
  Rat   688 IQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQ 752

  Fly   619 MNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIV 683
            |||||||.||.|.||.|.:|:.|:|.|||||....||..||||||.:.::|||:.|.|...:|||
  Rat   753 MNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIV 817

  Fly   684 NGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQ----FEMVCGNRIKISYI 744
            :.|......:||.|.:.|..||..|::||||:.||||.|..||||||    ||.......|:...
  Rat   818 DSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDNYHPKMVVF 882

  Fly   745 VVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNY 809
            ||||:|:|.::..:..|...|.||||||..||.....||:|::..||||...||||:.:.:..|.
  Rat   883 VVQKKISTNLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANL 947

  Fly   810 GPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL 867
            .||.:|:|::|||.:|:||.||:|:||.|.||||||:|.||.:..:.|..|.|.||:|
  Rat   948 SPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCENLFFL 1005

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	49/141 (35%)
AGO3	NP_001036627.2	Piwi_piwi-like_Euk	412..850	CDD:240016	182/443 (41%)
Piwil2	XP_006252333.2	ArgoN	250..>346	CDD:293095	30/96 (31%)
		ArgoL1	371..418	CDD:285861	16/46 (35%)
		PAZ_piwi_like	419..536	CDD:239211	47/122 (39%)
		Piwi_piwi-like_Euk	544..988	CDD:240016	182/443 (41%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166340834
Domainoid	1	1.000	310	1.000	Domainoid score	I1281
eggNOG	1	0.900	-	-		E1_KOG1042
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H23071
Inparanoid	1	1.050	615	1.000	Inparanoid score	I865
OMA	1	1.010	-	-		QHG54391
OrthoDB	1	1.010	-	-		D220258at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm44598
orthoMCL	1	0.900	-	-		OOG6_100694
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X548
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	12	11.670