Protein Alignment AGO3 and Ago4

DIOPT Version :10

• Sequence 1: NP_001036627.2
• Gene: AGO3 / 3355150
• FlyBaseID: FBgn0250816
• Length: 867
• Species: Drosophila melanogaster
• Sequence 2: NP_001381300.1
• Gene: Ago4 / 298533
• RGDID: 1304583
• Length: 861
• Species: Rattus norvegicus
• Alignment Length: 881
• Identity: 200/881 - (22%)
• Similarity: 351/881 - (39%)
• Gaps: 196/881 - (22%)

  Fly   122 GSKGSSVNIYCNYLKLTTDESKGVFNYEVRFFPP----------IDSV--HLRIKYLNDHKDKLG 174
            |:.|..:.:..|:.::...:. .|::|:|...|.          :|::  |.:::...|.:....
  Rat    22 GTVGKPIRLLANHFQVQIPKI-DVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYD 85

  Fly   175 GTKTFDGNTLY----LPI---------LLPN-------KMTVFISKAEDVELQIRILYKKKEEM- 218
            |.:     .:|    |||         .||.       |::|.......::|.:..|.....|:
  Rat    86 GKR-----NMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLSEVP 145

  Fly   219 RNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPL-AKLEVWPGYVTAVDEYKGGLMLCCDV 282
            .:..|..::: .|.:..:.|....|..|.|......|| ...|||.|:..:|......:||..||
  Rat   146 DDSVQALDVI-TRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDV 209

  Fly   283 SHRILCQ-KTVLEMLVDLYQ-QN--------------------------VEHYQESARKMLVGNI 319
            |.....: :.::|.:.::.. ||                          |.|..:..||..|.|:
  Rat   210 SATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNV 274

  Fly   320 VLTRYNNRTYKINDICFDQNPTCQFEIKTG----CTSYVEYYKQYHNINIKDVNQPLIY--SIKK 378
            .....:::|:.:             :::.|    || ..:|:||.:|:.:|..:.|.:.  ..:|
  Rat   275 TRRPASHQTFPL-------------QLENGQAMECT-VAQYFKQKYNLQLKYSHLPCLQVGQEQK 325

  Fly   379 SRGIPAERENL---QFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVS 440
            ...:|.|..|:   |.|:            :..:||:....|....|.:|: ||..:::..::.|
  Rat   326 HTYLPLEVCNIVAGQRCI------------KKLTDNQTSTMIKATARSAPD-RQEEISRLVKSNS 377

  Fly   441 NTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVT----------N 495
            ........|..:|:.:.|...::.||.:....:.:      .||    :|...|          .
  Rat   378 VLGGPDPYLKEFGIVVHNEMTELMGRVLPAPMLQY------GGR----NKTVATPNQGVWDMRGK 432

  Fly   496 EMLKVVHLSKWIIIHLRNYRQAATSLL----DNMKQACESLGMNISNPTMI---SLDHDRIDAYI 553
            :....:.:..|.:......:|....||    |.:::..:..||.|......   :...|.::...
  Rat   433 QFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMF 497

  Fly   554 QALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIR-SVVQKIVL 617
            :.|:... :..|::|.|... :...||.:|::..:.:.:.:|.:..|   |.:|.. ..:..:.|
  Rat   498 KHLKMTY-VGLQLIVVILPG-KTPVYAEVKRVGDTLLGMATQCVQVK---NVVKTSPQTLSNLCL 557

  Fly   618 QMNCKLGGSLWTVKIPFKN-------VMICGIDSYHDPSNRGN--SVAAFVASINSSYSQWYSKA 673
            ::|.|||| :..|.:|.:.       |:..|.|..|.|:..|.  |:||.|.|::...|::.:..
  Rat   558 KINAKLGG-INNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATV 621

  Fly   674 VVQTKREEIVNGL-----------SASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYE 727
            .|||.|:||...|           |.:.|:.::.|:....| ||.||.||.|:.:||:......|
  Rat   622 RVQTSRQEIAQELLFSQEVVQDLTSMARELLIQFYKSTRFK-PTRIIYYRGGVSEGQMKQVAWPE 685

  Fly   728 IPQFEMVC-----GNRIKISYIVVQKRINTRIFSG--------SGIHLENPLPGTVVDQHITKSN 779
            :......|     ..|..|:|||||||.:||:|..        ||    |...||.||..||..:
  Rat   686 LIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSG----NVPAGTTVDSTITHPS 746

  Fly   780 MYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKL 844
            .:||:|.|....|||..|:||.||.||..:..|.:|.|:|:||..|.....:|.|||...||..:
  Rat   747 EFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLV 811

  Fly   845 AY------------------LIGQSIQRDVAEALSE 862
            |:                  :.|||..|| .:||::
  Rat   812 AFRARYHLVDKDHDSAEGSHVSGQSNGRD-PQALAK 846

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	29/169 (17%)
		Piwi_piwi-like_Euk	412..850	CDD:240016	126/506 (25%)
Ago4	NP_001381300.1	ArgoN	26..155	CDD:465134	21/134 (16%)
		ArgoL1	166..216	CDD:462567	15/49 (31%)
		PAZ_argonaute_like	217..337	CDD:239212	23/133 (17%)
		Piwi_ago-like	384..819	CDD:240015	120/455 (26%)