Protein Alignment AGO3 and Ago3

DIOPT Version :10

• Sequence 1: NP_001036627.2
• Gene: AGO3 / 3355150
• FlyBaseID: FBgn0250816
• Length: 867
• Species: Drosophila melanogaster
• Sequence 2: NP_700451.2
• Gene: Ago3 / 214150
• MGIID: 2446634
• Length: 860
• Species: Mus musculus
• Alignment Length: 883
• Identity: 205/883 - (23%)
• Similarity: 350/883 - (39%)
• Gaps: 201/883 - (22%)

  Fly   121 YGSKGSSVNIYCNYLKLTTDESKGVFNYEVRFFPP----------IDSV--HLRIKYLNDHKDKL 173
            ||:.|..:.:..|..::...:. .|:.|||...|.          :||:  |.::....|.:...
Mouse    23 YGTMGKPIKLLANCFQVEIPKI-DVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVY 86

  Fly   174 GGTKT-FDGNTL-------YLPILLPN--------KMTV-FISKA---------------EDVEL 206
            .|.:: :..|.|       .|.:.||.        |::| |:|:.               |.:||
Mouse    87 DGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSVKFVSRVSWHLLHEALAGGTLPEPLEL 151

  Fly   207 QIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPL-AKLEVWPGY----- 265
            .       |....|.....:::. |.:..:.|....|..|........|| ...|||.|:
Mouse   152 D-------KPVSTNPVHAVDVVL-RHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVR 208

  Fly   266 -----------VTAVDEYKGG--LMLCCDV---------------SHRILCQKTVLEMLVDLYQQ 302
                       |:|...||..  :...|:|               |||:...|.:..:.|:
Mouse   209 PAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVE---- 269

  Fly   303 NVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCT---SYVEYYKQYHNIN 364
             |.|.....||..|.|:.....:::|:.:             :::.|.|   :..:|:::.:.:.
Mouse   270 -VTHCGTMRRKYRVCNVTRRPASHQTFPL-------------QLENGQTVERTVAQYFREKYTLQ 320

  Fly   365 IKDVNQPLIY--SIKKSRGIPAERENL---QFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVS 424
            :|..:.|.:.  ..:|...:|.|..|:   |.|:            :..:||:....|....|.:
Mouse   321 LKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCI------------KKLTDNQTSTMIKATARSA 373

  Fly   425 PNQRQMALNKFYE--NVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAE 487
            |: ||..:::...  |....|..||    :...:.:....::||.:....:.:.    ...|:..
Mouse   374 PD-RQEEISRLVRSANYETDPFVQE----FQFKVRDEMAHVTGRVLPAPMLQYG----GRNRTVA 429

  Fly   488 FSKHAVTNEMLKVVH----LSKWIIIHLRNYRQAATSLL----DNMKQACESLGMNISNPTMI-- 542
            ...|.|.:...|..|    :..|.|......||....:|    |.:::..:..||.|......
Mouse   430 TPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCK 494

  Fly   543 -SLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDL 606
             :...|.::...:.| :|.....|:::.|... :...||.:|::..:.:.:.:|.:..|   |.:
Mouse   495 YAQGADSVEPMFRHL-KNTYSGLQLIIVILPG-KTPVYAEVKRVGDTLLGMATQCVQVK---NVI 554

  Fly   607 KIR-SVVQKIVLQMNCKLGGSLWTVKIPFKN-------VMICGIDSYHDPSNRGN--SVAAFVAS 661
            |.. ..:..:.|::|.|||| :..:.:|.:.       |:..|.|..|.|:..|.  |:||.|.|
Mouse   555 KTSPQTLSNLCLKINVKLGG-INNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGS 618

  Fly   662 INSSYSQWYSKAVVQTKREEIVNGLSASF-EIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLN 725
            :::..|::.:...||..|:||:..|::.. |:.::.|:....| ||.||.||||:.:||....|.
Mouse   619 MDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFRQVLY 682

  Fly   726 YEIPQFEMVCGNRIK-----ISYIVVQKRINTRIFSG--------SGIHLENPLPGTVVDQHITK 777
            ||:......|.:..|     |:|||||||.:||:|..        ||    |...||.||..||.
Mouse   683 YELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSG----NIPAGTTVDTDITH 743

  Fly   778 SNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAH 842
            ...:||:|.|....|||..|:||.||.||..:..|.:|.|:|:||..|.....:|.|||...|||
Mouse   744 PYEFDFYLCSHAGIQGTSRPSHYHVLWDDNFFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAH 808

  Fly   843 KLAY------------------LIGQSIQRDVAEALSE 862
            .:|:                  :.|||..|| .:||::
Mouse   809 LVAFRARYHLVDKEHDSAEGSHVSGQSNGRD-PQALAK 845

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	23/141 (16%)
		Piwi_piwi-like_Euk	412..850	CDD:240016	129/492 (26%)
Ago3	NP_700451.2	ArgoN	73..167	CDD:465134	17/100 (17%)
		ArgoL1	177..227	CDD:462567	10/49 (20%)
		PAZ_argonaute_like	228..348	CDD:239212	23/137 (17%)
		Piwi_ago-like	393..818	CDD:240015	123/443 (28%)
		Interaction with guide RNA. /evidence=ECO:0000250|UniProtKB:Q9H9G7	530..567		7/39 (18%)
		Interaction with guide RNA. /evidence=ECO:0000250|UniProtKB:Q9H9G7	758..805		21/46 (46%)