Protein Alignment AGO3 and sago-2

DIOPT Version :10

• Sequence 1: NP_001036627.2
• Gene: AGO3 / 3355150
• FlyBaseID: FBgn0250816
• Length: 867
• Species: Drosophila melanogaster
• Sequence 2: NP_490758.1
• Gene: sago-2 / 171653
• WormBaseID: WBGene00018921
• Length: 887
• Species: Caenorhabditis elegans
• Alignment Length: 901
• Identity: 183/901 - (20%)
• Similarity: 323/901 - (35%)
• Gaps: 254/901 - (28%)

  Fly   125 GSSVNIYCNYLKLTTDESKGVFNYEVR---FFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYL 186
            |:.|||..|..|||...::.::.|.|:   .|...|.....|:.....|.   ||:..:..|
 Worm    38 GTKVNIDTNIRKLTIKPNQPIYKYAVQVNYVFRKPDGTEATIEMSKSAKK---GTEHDNDKT--- 96

  Fly   187 PILLPNKMTVFISKAEDVELQIRILYKKKEEMRNCTQLYN-----ILFDRVMKVLNY--VKFDRK 244
              ...|.....|.:.::::......|.::..:...|:|.|     ::.|::.|..|:  .:|..|
 Worm    97 --RCQNVYNEAIKRYDELKTGGPFFYDRQASLYTLTKLKNESISFVVTDKICKRQNFKEAQFVLK 159

  Fly   245 QFDP--------------SRPKIIPLAKLEVW------PGY-----------------VTAVD-- 270
            :.|.              |.|.......||..      |.:                 .|.||
 Worm   160 KVDQSFQSTSNDVIKTTNSCPANADKTLLEAMNIIVSGPAFENKNVITVGACVHYLIDPTGVDVA 224

  Fly   271 --EY-KGGLMLCCDVSHRILC----------------QKTVL-----EMLVDLYQ---------- 301
              || :|.|.....||..:..                .||.|     ..||:|.|
 Worm   225 YKEYPEGQLYSGVGVSKSVKTLEGTDKKVPSLFMTTEMKTTLFHPDYAPLVELLQTFRGFSTTLK 289

  Fly   302 ------QNVEHYQESARKMLVGNIVLTRY--------NNRTYKINDICFDQNPTCQFEIKTGC-- 350
                  |.:|       |..||..|:..|        :....||.........|| ||::...
 Worm   290 ANSPAAQRIE-------KAFVGLDVVLNYGVHKGLGEDGVVMKIRRFHTSAKETC-FEVEKSTRE 346

  Fly   351 -TSYVEYYKQYHNINIKDVNQPLIYSI--KKSRG---IPAERENLQFCLIPELCYLTGLRDEVRS 409
             |:..:|:|:.:.|.:|   .|.:::|  |..:|   .|||        :..||    ....|.:
 Worm   347 FTNVFDYFKKKYGITLK---YPDLFTIEAKGKQGKIHFPAE--------VLLLC----PNQTVTN 396

  Fly   410 DNKLMREIATFTRVS---PNQRQMALNKFYENV---SNTPAAQEILNSWGLSLTNNSNKISGRQM 468
            |..:..|.|...::|   |:.|:...:....||   ||        |.:|.....:...:.|..:
 Worm   397 DQMINNEQADMIKMSAAQPHIRKTTTDTIVRNVGLASN--------NIYGFIKVEDPVNLEGMVL 453

  Fly   469 DIEQIYF--SKISVSAGRSAEF-SKHAVTNEMLKVVHLSKWIIIHLRN------YRQAATSLLDN 524
            ...:|.|  ::::..|...:.| :......:......|:||.::.::|      .:|.|..:::|
 Worm   454 PKPKIAFAGNRLADLANPKSRFPTDFNRAGQYYDAKELTKWELVFVQNEEVQGLAKQLADEMVNN 518

  Fly   525 MKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSE 589
                    ||..|||||..:....::...:..:   ...||::..:..:|.:  |....|....:
 Worm   519 --------GMKCSNPTMSFIIRGDLEPIFKKAK---AAGTQLLFFVVKSRYN--YHQQIKALEQK 570

  Fly   590 IPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNS 654
            ..:.:|.|.|:|.:...:.......|:.:.|.||||..:.:          |.::::.| ||  .
 Worm   571 YDVLTQEIRAETAEKVFRQPQTRLNIINKTNMKLGGLNYAI----------GSEAFNKP-NR--L 622

  Fly   655 VAAFVASI----NSSY-------------SQWYSKAVVQTKREEIVNG--LSASFEIALKMYRKR 700
            :..||.|.    |..|             .|.::...|...|:..|.|  :..:.....|...::
 Worm   623 IVGFVTSQRVGGNPDYPISVGFAANMLKHHQKFAGGYVYVHRDRDVFGSIIKDTLLTIFKTCTEQ 687

  Fly   701 NGKLPTNIIIYRDGIGDGQLYTCLNYEI----------------PQFEMVCGNRIKISYIVVQKR 749
            .|: |.:|::|.:|:.:|| ::.:|.|.                |.|      |..|:.|...|.
 Worm   688 RGR-PDDILLYFNGVSEGQ-FSMINEEFSARVKEACMAFQKEGTPPF------RPHITIIASSKA 744

  Fly   750 INTRIFSGSGIHLENPLPGTVVDQHITKSNMY-DFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDI 813
            .|.|::......:.|..|||||| |...||:| :::..|.:.||||...|.:.:: .....||..
 Worm   745 HNERLYKSDKGRIVNLEPGTVVD-HTIVSNVYTEWYHASAVARQGTAKATKFTLI-FTTKAGPQA 807

  Fly   814 -----IQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAY-LIGQSIQRDVAEALSEK 863
                 :::|:..||                 |.|::.: .:|..:...:|:..|::
 Worm   808 EPLWHLEQLTNDLC-----------------YDHQIVFHPVGLPVPLYIADRYSQR 846

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	38/173 (22%)
		Piwi_piwi-like_Euk	412..850	CDD:240016	101/494 (20%)
sago-2	NP_490758.1	PAZ_argonaute_like	272..389	CDD:239212	29/135 (21%)
		Piwi-like	444..849	CDD:239208	93/456 (20%)