DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nipped-B and mis4

DIOPT Version :9

Sequence 1:	NP_001036451.1	Gene:	Nipped-B / 3355136	FlyBaseID:	FBgn0026401	Length:	2077	Species:	Drosophila melanogaster
Sequence 2:	NP_592917.2	Gene:	mis4 / 2542657	PomBaseID:	SPAC31A2.05c	Length:	1587	Species:	Schizosaccharomyces pombe

Alignment Length:	1862	Identity:	370/1862 - (19%)
Similarity:	705/1862 - (37%)	Gaps:	448/1862 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly   267 FPQEPSNFNLNSVNIPNMLY--VQSPPFTESEPTQAHHSSIEYLKKK-KSQILDV-----SILNR 323
            |...|..|  ..||..|.:.  :|..|...|.|.:....::.|...| :::.|.:     ||:.|
pombe     3 FEMTPETF--KKVNSSNRIIKGLQYTPLASSIPLENGLQNVLYPSSKFQNEPLQLNSEESSIMQR 65

  Fly   324 QQVLENTSLHIINKSSTYQTNQNVSTTTSTS-------TSSSGKSQVRVC----INRLSIEDSRL 377
            ...:.|.....:|.|.|:...:|..:...|.       .::|.....:||    ::.|:.....|
pombe    66 YVDMLNPGATFVNDSETFNFYKNALSAMITEPAMPAMRVNASPVLDQKVCNSDSLSELNDFTKSL 130

  Fly   378 MQQSIKKFVQKSPELARSMGLLQETCQQQHENLNIIPTSAEECVLESRASSTNNTVKIPIDTFST 442
            :|.|:.....|....|.|:    .|.:...:|..:.|:|:......||             .|:.
pombe   131 IQPSMLMCEPKEKPDASSI----NTNRSSSDNGFLTPSSSPRSPSCSR-------------VFNA 178

  Fly   443 IKADEKRSAKRKLAISIGDIPPEQIFSKPKMRRVERITPLSNTKCVKEEVTRSQTYQQ-FIRNMD 506
            ::....:.:|       .||      :.||.|.:|      :|...:|..::.|..|. .::.:.
pombe   179 VQLCSPKKSK-------DDI------TTPKKRLME------DTYSPRESPSKIQRLQDVLLKQLQ 224

  Fly   507 H----IIEILDDSESPNFDSEDV-----DETIECISSKLLNSMSTDVAKLKAKQALDSIPKNKLT 562
            .    |.::::...|.:|.|..:     |:..:.|||..:..:...:.||....|.|.:.:....
pombe   225 DTRLLISQVIEAENSEDFSSNSLFIKREDDDGKHISSHAIEKLYMALTKLSRLGACDKLLEEGSI 289

  Fly   563 LLINYAMRNVYLARNYFAGTEDEDEFVDDEVIEKLLNAMD-ACLLICNIYSTVSDLQFLQEDNVS 626
            :|:.                         :::||.|..:. ||..|.|::.:::     |..|:.
pombe   290 ILVK-------------------------QILEKELKELPVACYSIINLHDSLT-----QFPNLD 324

  Fly   627 HIIK--------------FTQFQLRETIFPLHDPV-----YTA-KSIKRTTHRKKIKSHQAQNRS 671
            .|:|              |.:.|..|:|..|.:.:     ||. ::|......|...:..::.:.
pombe   325 FILKTTALVLFIIFLVPSFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQH 389

  Fly   672 MQLFYLKTVELLKVFVTLFDKCVFVDTIVLPLSTLAIEPFFVDN--------------------- 715
            :.:...|...:|.:...:..:....:.:|:.:..|..:...:||
pombe   390 LSVLLQKVANVLNILSKVAHEIPLSEAVVIRIVYLFPKVSTLDNSFKTKLPNCNSSSFDFLKAPL 454

  Fly   716 IETLQFVCLELVTTIFRKERYDKIRNSILGDILTSIDRLPSSKKNLRPYKLTNNGGNIQMVTALV 780
            .:|||:        :||...|.  |:.|:.:.||:...||:::...|.|:| :||.:||..:.|.
pombe   455 FQTLQY--------LFRLYPYQ--RDFIIEESLTNFSHLPTARSVSRTYRL-SNGKSIQYYSTLF 508

  Fly   781 LQLIQCATI--LPDS--LCDNGKFSNKPQEGNTFD--EEGKKLLQPSQDLLVLQKYDVAVSIGGN 839
            ::|||..:|  |.||  :....|.:.....||..:  :..:.:|..|:.    ::|.:|..|...
pombe   509 VRLIQSCSIQNLFDSEIVQSESKSTEALHSGNLTEHLKTVESILSKSRH----EEYRIANHIVAY 569

  Fly   840 FLTTFLNKCKSRSNETDFRPLFENFIHDLLATVNKPEWPASELLLSLLGTMLVRYVSDKGIEQSI 904
            .|:..|.:.|:.| :..|..|.:..:.|||..::.|||..:|.::......||..|::.....|.
pombe   570 LLSRSLKQNKTES-DNSFAILTKILLEDLLNMLSLPEWCGTETIIRQFAMNLVMTVTNDKQAVSS 633

  Fly   905 RLVSLDYLGIVAARLRKDTVESRCRVNIIDSMIQSIKLEQEKEGDVTSNNDQFDLEPEEQRTDFL 969
            :..:||.:.::              ||.:.::......|:......|:.||.....|...|.:.|
pombe   634 KNAALDLISLI--------------VNKVLALFDLSLFEKHNIPAPTNFNDIISFIPSITRLNEL 684

  Fly   970 QKILLD---FLAV-NAQEENLI-WDYARHFYLAQWYRDVIYQRRRINDGKKGLAFRKSKIRNNRR 1029
            .::..:   ||.. :...||:: ::|.:      |:..::..|:..|| .:.|....:.|..|.:
pombe   685 SQVSFNHFYFLCKGDISLENILPYNYNK------WFSFLLQLRKVCND-SEALKIIDNCIDKNMQ 742

  Fly  1030 TNGDYLDTSDSGSCDESDTDTNKKRIHCVDSNDFELNINIYKALEARKQYFINKIKPFSVFGEQN 1094
            .:.:..........||:|.|     |..:......|.:|:        ::|::.|..|
pombe   743 KSQEGFQGPSPFKADENDED-----IFIISLYHSSLFLNL--------KFFVSLIIGF------- 787

  Fly  1095 HSSNQHIKTYIDYNNAQLIAQYLATKRPFSQSFDGCLKKIILVVNEPSIAVRTRAMKCLANIVEV 1159
                      :|...|.     |.||         ||:    ::|:         ||.:.:|:..
pombe   788 ----------LDSPQAS-----LRTK---------CLR----IINQ---------MKTIPSILRT 815

  Fly  1160 DPLVLKRKDMQMGVNQKFLDTAISVREAAVDLVGKFVLSNQDLIDQYYDMLSTRILDTGVSVRKR 1224
            .|.||.:      :..|..|.:..||:..:||:|.::::.::.|.|.|..:.:.|.|....||||
pombe   816 HPEVLAQ------IISKSNDQSAIVRDTVLDLLGTYIMAYRETIPQIYGCIISGISDPSTIVRKR 874

  Fly  1225 VIKILRDICLEYPDFSKIPEICVKMIRRVHD-EEGIQKLVTEVFMKMWFTP------CTK----- 1277
            .||.|.::.....|.:...:|..|::.|.:| ||.|.:|..||..|:||:|      |.|
pombe   875 AIKQLCEVYEATEDLNIRVDIASKLLTRSNDEEETISELSLEVLEKLWFSPASNELDCQKGYEQL 939

  Fly  1278 ----NDKIGIQRKINHIIDVVNTAHDTGTTWLEGLLMSIFKPRDNMLRSEGCVQEFIKKNSEPPM 1338
                ..|:.:|     ...::....:..|. ...||::..|   .||.|:   :|.........:
pombe   940 TFLEKQKLRVQ-----YFPILKLCAEPSTE-RHVLLVTSLK---TMLTSK---EEINLSTLHTQI 992

  Fly  1339 DIVIACQQLADGLVDRLIEL--EDTDNSRMLGCI----TTLHLLAKVRPQLL-VKHAITIEPYLN 1396
            .::::|      |.::|||:  ||..:....|.:    :||.:.::..|.|. :.:...::|||.
pombe   993 RLLLSC------LFNQLIEVVTEDQVDESTKGILYEIMSTLFVFSRAFPFLFDLSYLHLLKPYLR 1051

  Fly  1397 IKCHSATAAKFICAVADILEKVVPLVNNASESFLASLEEHLMLLVVSRNQAEVTSCVSCLGAL-- 1459
             ...:....:|:..|..|..:|:|.....|||||.|||..|:..:.....|.:...|.||.:|
pombe  1052 -SASTIEEQRFLYYVVAIFRQVLPFQKEISESFLRSLESVLLQRLTKAGTATLMEIVPCLCSLFT 1115

  Fly  1460 -VNKITHNFKLIRDC-------------FQKFYRVLDVSRSQVIQGNNSVDNIYTPSFRRSLFTI 1510
             :|......|::..|             |||..|::|:                          |
pombe  1116 RLNDYERLKKIVVSCLKSLEEARHSENNFQKMVRLIDL--------------------------I 1154

  Fly  1511 GILMRYFDFKSPIALGETNDGLPVSI------CEDVFHCLM-FFCRCTNQ---EIRKQALISLGS 1565
            |:..||.|      |...||....|:      |:|.:..|: :|.:....   ::|...:.::..
pombe  1155 GLFSRYGD------LNRINDDWKHSLDFISPECDDAYVILLGYFQKLLKDAKGQLRIHIIDNMSR 1213

  Fly  1566 FCVLNDG-YLTRSELKNLYCEILSSIANDAG--FK----IICMRNIWIYLTESEMFMHNKEKEWE 1623
            .|:.... :::...|..|...|..:..|:..  ||    ::......|:..:.::.:..|:....
pombe  1214 ICLRETSLFISPLMLSTLDMIIAENNVNEVSVLFKSFLELLAADEDLIFEADQKLSLKGKQNVQS 1278

  Fly  1624 KQSKHED-LKEMNDVS--SGMASRIIQLYLEEILECFLNRDDTVRLWAVKVIQIVLRQGLVHPVR 1685
            .:|...| ||...|..  .|:::.::|.:|..||:...:::....:..:::::.::.||||:|..
pombe  1279 NKSVDRDMLKGTKDKQWIEGVSASLMQHFLPCILDSCFSKNLRYSMLGIEILKCIIHQGLVNPRM 1343

  Fly  1686 MVPYLICLSTDHRIESAHRADALLKDIDKTYSGFVNMKVQFGLQLCFKLQKILQINNRGKLEIIR 1750
            ....:|.|.::...|:...|..|..::.:.:...::........|.|.|||.
pombe  1344 CFSTIIALESNAIKETREVAILLHTELHRRHESLIDGLYAQSADLIFSLQKT------------- 1395

  Fly  1751 GYASRGPDNTTTALNDF-----LYTLLRTTK--PQRRALVQTVTK----------QFDDQKTSLQ 1798
                  .:..|..|.:|     .||::...|  ..|:.|:..:.|          .|.::|.|..
pombe  1396 ------EEYQTFKLGEFSPFQSAYTIVSADKSSKSRKKLIMQILKPLKLDGIDLPSFTEEKVSFV 1454

  Fly  1799 QMLYIADNLAYFPYVVQDEPLYLIHQIDLLISMAGTHLLATFK--EHIKPSDKEGDVLEDDDDVE 1861
            ....:.  ||..|||..:|||.:|..:|.:::..|..:....|  :|
pombe  1455 SFCCVC--LAGIPYVSIEEPLMIISTVDSVLATIGPTITGWMKKLDH------------------ 1499

  Fly  1862 DPEVLFNRLPEDLTEIIKCITSAQACMLLLILKDHLKEMYAITDSKISRYSPSEQK----LYEKA 1922
                          |..|.:.....|. |:.||.::|..::|:||  ||  |..:|    |..:.
pombe  1500 --------------ERFKILAGINLCN-LIYLKRYIKYAFSISDS--SR--PIREKKPLTLLNRG 1545

  Fly  1923 VTRKSVNDFNPKTTIDVIKKQMSQEKLSTDINFTLTKEEKLD 1964
            ......:|..|.....::.|...:|.:       |:.|::::
pombe  1546 YVDLITSDAKPDIVSKLVIKLFEEENI-------LSGEDQVE 1580

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nipped-B	NP_001036451.1	Cohesin_HEAT	1151..1192	CDD:289531	10/40 (25%)
Nipped-B	NP_001036451.1	Nipped-B_C	1643..1826	CDD:289591	40/199 (20%)
mis4	NP_592917.2	HEAT repeat	780..808	CDD:293787	12/71 (17%)
		Cohesin_HEAT	801..842	CDD:289531	12/59 (20%)
		HEAT repeat	818..846	CDD:293787	9/33 (27%)
		HEAT repeat	855..881	CDD:293787	11/25 (44%)
		HEAT repeat	892..921	CDD:293787	10/28 (36%)
		Nipped-B_C	1301..1480	CDD:289591	40/199 (20%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	69	1.000	Domainoid score	I2687
eggNOG	1	0.900	-	-		E1_KOG1020
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	148	1.000	Inparanoid score	I1321
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003400
OrthoInspector	1	1.000	-	-		oto102035
orthoMCL	1	0.900	-	-		OOG6_103308
Panther	1	1.100	-	-	LDO	PTHR21704
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R719
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	9.890