DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Gprk1 and Grk5

DIOPT Version :9

Sequence 1:	NP_001036438.1	Gene:	Gprk1 / 3355013	FlyBaseID:	FBgn0260798	Length:	700	Species:	Drosophila melanogaster
Sequence 2:	NP_061357.3	Gene:	Grk5 / 14773	MGIID:	109161	Length:	590	Species:	Mus musculus

Alignment Length:	595	Identity:	213/595 - (35%)
Similarity:	314/595 - (52%)	Gaps:	71/595 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 DLEAVLADVSYLMAME------KSKCTPAARASKK----LNLPDPSVRSVMYKYLEKEGELNFHK 57
            :||.::|:...|.|.|      |.|       |||    |..|..|....:.:.::::    ::.
Mouse     2 ELENIVANTVLLKAREGGGGKRKGK-------SKKWKEILKFPHISQCEDLRRTIDRD----YYS 55

  Fly    58 IFN-EVLGYLLFKDFCENDSEEPIQQ--LKFFEQIKLFEKTECYDER-KKMARDIYDNFIMEEML 118
            :.: :.:|.|||:.|||.   .|..:  ::|.:.:..:|.|.  ||. ....::|...::..:..
Mouse    56 LCDKQPIGRLLFRQFCET---RPGLECYIQFLDLVAEYEITP--DENLGAKGKEIMTKYLTPKSP 115

  Fly   119 SHTYEYSKHAVASVQKYLLKNEVPVDLFEPYLEEIFTQLKGKPFKKFLESDKFTRFCQWKNLELN 183
            ....:..:..|:..:|.||::... :||....:.:...|||.||.::|:|..|.||.|||.||..
Mouse   116 VFIAQVGQDLVSQTEKKLLQSPCK-ELFSACAQSVHDYLKGDPFHEYLDSMYFDRFLQWKWLERQ 179

  Fly   184 IQLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGEMLALNERNMLQAVST 248
             .:|.|.|..:|::|:||||||..|:...||||||.|.|:|||||.::||.:||||:.:|:.|::
Mouse   180 -PVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNS 243

  Fly   249 GIDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGI--FSEDEMKFYAAEVILGLEHMHK 311
                .|:|.:.||:.|.|.||.:|.:||||||.:|:...|.  |.|:...|||||::.|||.:|:
Mouse   244 ----QFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHR 304

  Fly   312 RCIVYRDLKPANILLDENGHIRISDLGLACDFSKKKPHAS-----VGTHGYMAPEVLSKGTSYDS 371
            ...|||||||.|||||:.|||||||||||.    |.|...     |||.||||||||: ...|..
Mouse   305 ENTVYRDLKPENILLDDYGHIRISDLGLAV----KIPEGDLIRGRVGTVGYMAPEVLN-NQRYGL 364

  Fly   372 CADWFSFGCMLYKLLKGHSPFRQHKTKDKL-EIDKMTLTMNVELPESFSLELKNLLEMLLQRDVS 435
            ..|::..||::|::::|.||||..|.|.|. |:|:..|.........||.|.|::..|||.:|..
Mouse   365 SPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSICNMLLTKDSK 429

  Fly   436 KRLGCMGNGADEVKMHNFFCGIDWHQVYIQKYTPPLVPPRGEVNAADAFDIGSFDEEDTKGIKLN 500
            :||||...||.|||.|.||..:::.::......||.||....|...|..||..|  ...||:.|:
Mouse   430 QRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQF--STVKGVNLD 492

  Fly   501 DADQDLYKMFSL-TISERWQQEVSET-------VFDTVNTETDKLEQKR-----------KLKQK 546
            ..|.|.|..||. ::...||.|:.||       ||....|.:..|.:.:           :|.::
Mouse   493 HTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRR 557

  Fly   547 QHFDADEKES 556
            || .::.|.|
Mouse   558 QH-QSNSKSS 566

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Gprk1	NP_001036438.1	RGS_GRK2_GRK3	30..186	CDD:188701	40/163 (25%)
		PKc_like	185..531	CDD:304357	157/361 (43%)
		Pkinase	191..454	CDD:278497	129/270 (48%)
		PH_GRK2_subgroup	552..675	CDD:269946	2/5 (40%)
		PH	559..657	CDD:278594
Grk5	NP_061357.3	N-terminal	1..185		50/200 (25%)
		Interaction with calmodulin. /evidence=ECO:0000250	20..39		7/25 (28%)
		RGS	53..175	CDD:295367	32/127 (25%)
		STKc_GRK5	183..495	CDD:270780	144/322 (45%)
		S_TKc	186..448	CDD:214567	129/270 (48%)
		Nuclear localization signal. /evidence=ECO:0000250	388..395		3/6 (50%)
		Sufficient for membrane localization. /evidence=ECO:0000250	546..565		3/19 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	557..590		4/11 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167845431
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0986
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1104340at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.750