Protein Alignment Set1 and Setd1a

DIOPT Version :10

• Sequence 1: NP_001015221.1
• Gene: Set1 / 3354971
• FlyBaseID: FBgn0040022
• Length: 1641
• Species: Drosophila melanogaster
• Sequence 2: NP_821172.2
• Gene: Setd1a / 233904
• MGIID: 2446244
• Length: 1716
• Species: Mus musculus
• Alignment Length: 1938
• Identity: 488/1938 - (25%)
• Similarity: 760/1938 - (39%)
• Gaps: 543/1938 - (28%)

  Fly    18 DSSLANSKMPRNFKLL----SDPQLVKCGTRLYRYDGL--MPGDPSYPTITP-RDPRNPLIRIRA 75
            |...|.|...||:||:    .||.|.:...::|||||:  ...|..|   || .|.::|...:|:
Mouse     8 DGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVSDSKY---TPVEDLQDPRCHVRS 69

  Fly    76 RAVEPLMLLIPRFVIDSDYVGQPPAVEVTIVNLNDNIDKQFLASMLDKCGTSDEINIYHHPITNK 140
            :| ....|.:|:|.:|..|:||.|..|||...||||:.:.||..|..|.|..:|:.|..||.|.|
Mouse    70 KA-RDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRK 133

  Fly   141 HLGIARIVFDSTKGARQFVEKYNQKSVMGKILDVFCDPFGATLKKSLESLTNSVAGKQLI---GP 202
            |||:||::|.||:||::.|:..:..||||.|:....|..|....|..|.:.|.....|.:   |.
Mouse   134 HLGLARVLFTSTRGAKETVKNLHLTSVMGNIIHAQLDIKGQQRMKYYELIVNGSYTPQTVPTGGK 198

  Fly   203 KVTPQWTFQQAALEDTE----------------FIHGYPEKNGEHIKDIYTTQTNHEIPNRSRDR 251
            .::.::....||.|.||                .:.|.| .||...               |:|.
Mouse   199 ALSEKFQGSGAAAETTEARRRSSSDTAAYPAGTTVGGTP-GNGTPC---------------SQDT 247

  Fly   252 NWNRDKERERDRHFKERSRHSSERSYDRDRGMRENVGTSIRRRRTFYRRRSSDISPEDSRDILIM 316
            |::             .||..:..|:.:                  :..:||..:|..||.
Mouse   248 NFS-------------SSRQDTPSSFGQ------------------FTPQSSQGTPYTSRG---- 277

  Fly   317 TRERSRDSDSRPRDYCRSRERESFRDRKRSHEKGRDQPREKREHYYNSSKDREYRGRDRDRSAEI 381
            :...|:||     .|..|....||:.| ||....:|.  ..|.|:..||............:|
Mouse   278 STPYSQDS-----AYSSSTTSTSFKPR-RSENSYQDS--FSRRHFSTSSAPATTATATSATAA-- 332

  Fly   382 DQRDRGSLKYCSRYSLHEYIETDVRRSSNTISSYYSASSLPIASHGF--NSCSFPSIENIKTWSD 444
                                .|....||::.||..|:||...::..|  :..|:|:.  .::|:.
Mouse   333 --------------------ATAASSSSSSSSSSSSSSSSSSSASQFRGSDSSYPAY--YESWNR 375

  Fly   445 RRAWTAFQP---------------------------------------DFHP---VQPPPPPPEE 467
            .:..|::.|                                       |:.|   ..|||.|||.
Mouse   376 YQRHTSYPPRRATREDPSGASFAENTAERFPPSYTSYLAPEPNRSTDQDYRPPASEAPPPEPPEP 440

  Fly   468 --------------------IDNWDEEEHDKNSIVPTHYGCMAKLQPPVPSNVNFATKLQSV--T 510
                                ..:.:.||    :..|......|:...|.|...|     :||  .
Mouse   441 GGGGGGSGGGGGGGGGGGGGAPSPEREE----ARTPPRPASPARSGSPAPETTN-----ESVPFA 496

  Fly   511 QPNSDPGTVDLDTRIALIFKGKTFGNAPPFLQMDSSDSE-------------------------T 550
            |.:|      ||:||.::.|.:.  :...||..|:.:.|                         |
Mouse   497 QHSS------LDSRIEMLLKEQR--SKFSFLASDTEEEEENSSAGPGARDAGAEVPSGAGHGPCT 553

  Fly   551 DQGKPEVFSDVNSDSNNSENKKRSCEKNNKVLHQPNEASDISSDEELIGKKDKSKLSLICEKEVN 615
            ....|..|.||....:......|...|.|    ..|:||..||.|::               |::
Mouse   554 PPPAPANFEDVAPTGSGEPGAARESPKAN----GQNQASPCSSGEDM---------------EIS 599

  Fly   616 DDNMS---------------------------LSSLSSQEDP----------------------- 630
            ||:..                           |:||.....|
Mouse   600 DDDRGGSPPPAPTPPQQPPPPPPPPPPPPPPYLASLPLGYPPHQPAYLLPPRPDGPPPPEYPPPP 664

  Fly   631 ---------------IQTKEGAEYKSIMSSYMYSHSNQNPFYYHASGYG---------------H 665
                           :..:.||::..:..|:..........:....|.|               .
Mouse   665 PPPPPHIYDFVNSLELMDRLGAQWGGMPMSFQMQTQMLTRLHQLRQGKGLTAASAGPPGGAFGEA 729

  Fly   666 YLSGIPSESAS-----RLFSNGAYVHSEYLKAVASFNFDSFSKPYDYNKGALSDQNDGIRQK--- 722
            :|...|.:.|:     .|::.|......|.:..........::|..  ..::|.:...:..:
Mouse   730 FLPFPPPQEAAYGLPYALYTQGQEGRGSYSREAYHLPLPMAAEPLP--SSSVSGEEARLPHREEA 792

  Fly   723 -------------VKQVIGYIVEELKQILKRDVNKRMIEITAFKHFETWWDEHTSKARSKPLFEK 774
                         |.:|:..:|:|:|.|::||:|::|:|..||..|:.||:....||  || |:
Mouse   793 EIAESKVLPSAGTVGRVLATLVQEMKSIMQRDLNRKMVENVAFGAFDQWWESKEEKA--KP-FQ- 853

  Fly   775 ADSTVNTPLNCIKDTSYNE-------KNPDINLLINAHRE--VADFQSYS-SIGLRAAMPKLPSF 829
                     |..|..:..|       |.|.:..|::..:.  :...:::: ..|||.|: :||||
Mouse   854 ---------NAAKQQAKEEDKEKMKLKEPGMLSLVDWAKSGGITGIEAFAFGSGLRGAL-RLPSF 908

  Fly   830 RRIRKHPSPI--------PTKRNFLERDLSDQEEMVQRSDSDKEDSNVEISDTARSKIKGPVPIQ 886
            :..||.||.|        |......|.|..|.|...:..:..:..:.....|..|.|.:|
Mouse   909 KVKRKEPSEISEASEEKRPRPSTPAEEDEDDPEREKEAGEPGRPGTKPPKRDEERGKTQG----- 968

  Fly   887 ESDSKSHT---SGLNSKRKGSASSFFSSSSSSTSSEAEYEAIDCVEK---ARTSEEDSPRGYGQR 945
             ...||.|   .|..:.::.|:.............|.:.||:|..:|   |...|::......|.
Mouse   969 -KHRKSFTLDSEGEEASQESSSEKDEDDDDEDEEDEEQEEAVDATKKEAEASDGEDEDSDSSSQC 1032

  Fly   946 NL-------NQRTTTIRNRNLVGTMDVINVRNLCSGSNEFKKENVTKRTKKNIYSDTDEDNDRTL 1003
            :|       |..|:...:                ..|:.....:.:..:..:..|.::|:....:
Mouse  1033 SLYADSDGENGSTSDSES----------------GSSSSSSSSSSSSSSSSSSESSSEEEEQSAV 1081

  Fly  1004 FP-ALKEKNISTILSDLEEISKDSCIGLDENGIEPTILRK-IPNTPKLNEECRRSLTPVPP--PG 1064
            .| |...:.:...|...:|  |....||.::.:.|...:: :|..|....|......|.||  |.
Mouse  1082 IPSASPPREVPEPLPAPDE--KPETDGLVDSPVMPLSEKETLPTQPAGPAEEPPPSVPQPPAEPP 1144

  Fly  1065 YNEEEIKKKVDCKQKPSFEYDRIYSDSEEEKEYQERRKRNTEYMAQMEREFLEEQ---------- 1119
            ....:...::|  ::||.....:        ...::|::...:.|..|....|..
Mouse  1145 AGPPDAAPRLD--ERPSSPIPLL--------PPPKKRRKTVSFSAAEEAPVPEPSTAAPLQAKSS 1199

  Fly  1120 ---EKRIEKSLDKNLQS------------PNNIVKNNNSPRNK-NDETRKTAISQTRSCFESASK 1168
               .:::.:.:::.:::            |..:.:..   ||: ....|.|   :.....||.::
Mouse  1200 GPVSRKVPRVVERTIRNLPLDHASLVKSWPEEVARGG---RNRAGGRVRST---EEEEATESGTE 1258

  Fly  1169 VDTTLVNIISVENDINEFGPHEEG-DVLTNGCNKMYTNSKGKTKRTQSPVYSEGGSSQASQASQV 1232
            ||..::..:::.       |...| ..|..|.:...|.:..:.:| .||:           .|.:
Mouse  1259 VDLAVLADLALT-------PARRGLATLPTGDDSEATETSDEAER-PSPL-----------LSHI 1304

  Fly  1233 ALEHCYSL----PPHS------------VSLGDYPSGKVNETKNILKREAENIAIVSQMTRTGPG 1281
            .|||.|:|    ||.:            .:|...|:.:|.|...::..|||...  .|:.:..|.
Mouse  1305 LLEHNYALAIKPPPTTPAPRPLEPAPALAALFSSPADEVLEAPEVVVAEAEEPK--QQLQQQHPE 1367

  Fly  1282 RPRKDPICIQKKKRDLAPRMSNVKSKMTPNGDEWPDLAHKNVHF-------------VPCDMYKT 1333
            :..      ::::.|......:.:|..:.:.||...:..:::..             .|...::.
Mouse  1368 QEG------EEEEEDEEEESESSESSSSSSSDEEGAIRRRSLRSHTRRRRPPLPPPPPPPPSFEP 1426

  Fly  1334 RDQNEEMVILYTFLTKGIDAEDINFIKMSYLDHLHKEPYAMFLNNTHWVDHCTTDRAFWPPPSKK 1398
            |.:.|:|.|||.....|:|.||:::::::|...|.:...|.:||:||||.|..|:.:   .|.:|
Mouse  1427 RSEFEQMTILYDIWNSGLDLEDMSYLRLTYERLLQQTSGADWLNDTHWVQHTITNLS---TPKRK 1488

  Fly  1399 RRKDDELIRHKTGCARTEGFYKLDVREKAKHKYHYAKANTEDSFNEDRSDEPTALTNHHHNKLIS 1463
            ||..|....|:||.||:||:|.:..:||.|    |.......:...:..|
Mouse  1489 RRPQDGPREHQTGSARSEGYYPISKKEKDK----YLDVCPVSARQLEGGD--------------- 1534

  Fly  1464 KMQGISR---EARSNQRRLLTAFGSMG--ESELLKFNQLKFRKKQLKFAKSAIHDWGLFAMEPIA 1523
             .||.:|   |.||.|||||:|.|:..  :|:|||.||||||||:|:|.:|.||:||||||||||
Mouse  1535 -TQGTNRVLSERRSEQRRLLSAIGTSAIMDSDLLKLNQLKFRKKKLRFGRSRIHEWGLFAMEPIA 1598

  Fly  1524 ADEMVIEYVGQMIRPVVADLRETKYEAIGIGSSYLFRIDMETIIDATKCGNLARFINHSCNPNCY 1588
            |||||||||||.||.:|||:||.:|...|||||||||:|.:|||||||||||||||||.|.||||
Mouse  1599 ADEMVIEYVGQNIRQMVADMREKRYVQEGIGSSYLFRVDHDTIIDATKCGNLARFINHCCTPNCY 1663

  Fly  1589 AKVITIESEKKIVIYSKQPIGINEEITYDYKFPLEDEKIPCLCGAQGCRGTLN 1641
            ||||||||:||||||||||||::|||||||||||||.|||||||.:.|||:||
Mouse  1664 AKVITIESQKKIVIYSKQPIGVDEEITYDYKFPLEDNKIPCLCGTESCRGSLN 1716

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Set1	NP_001015221.1	RRM_Set1	99..191	CDD:409745	39/91 (43%)
		U2AF_lg	255..>386	CDD:273727	24/130 (18%)
		N-SET	1352..1496	CDD:463344	49/148 (33%)
		SET_SETD1	1490..1637	CDD:380946	117/146 (80%)
Setd1a	NP_821172.2	Interaction with WDR82. /evidence=ECO:0000250|UniProtKB:O15047	60..89		8/29 (28%)
		RRM_Set1A	92..186	CDD:409964	39/93 (42%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	194..367		47/253 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	380..499		20/127 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	516..670		25/172 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	849..869		7/32 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	911..1206		55/328 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1230..1259		6/34 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1275..1297		5/22 (23%)
		HCFC1-binding motif (HBM). /evidence=ECO:0000250|UniProtKB:O15047	1307..1311		2/3 (67%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1355..1427		8/79 (10%)
		Interaction with CFP1. /evidence=ECO:0000250|UniProtKB:O15047	1424..1459		11/34 (32%)
		N-SET	1434..1571	CDD:463344	53/159 (33%)
		Interaction with ASH2L, RBBP5 and WDR5. /evidence=ECO:0000250|UniProtKB:O15047	1459..1546		32/109 (29%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1480..1508		12/30 (40%)
		WDR5 interaction motif (WIN). /evidence=ECO:0000250|UniProtKB:O15047	1501..1506		3/4 (75%)
		RxxxRR motif. /evidence=ECO:0000250|UniProtKB:P38827	1546..1551		3/4 (75%)
		SET_SETD1	1565..1712	CDD:380946	117/146 (80%)