DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Fign and SPAST

DIOPT Version :9

Sequence 1:	NP_001259995.1	Gene:	Fign / 33544	FlyBaseID:	FBgn0031519	Length:	523	Species:	Drosophila melanogaster
Sequence 2:	NP_055761.2	Gene:	SPAST / 6683	HGNCID:	11233	Length:	616	Species:	Homo sapiens

Alignment Length:	454	Identity:	167/454 - (36%)
Similarity:	261/454 - (57%)	Gaps:	33/454 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly    90 ESCQQTEMASSDLQAPALE--VSSTYPVQQAVKSRPEGQFPESR----NNST------------- 135
            |.|::...    |||..:.  |.:...:|...|.:|...|.:|:    |:||
Human   169 EQCERARR----LQAKMMTNLVMAKDRLQLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSES 229

  Fly   136 ----KKIDAQQYSSESSSQSGFGFRTAREQLIMDELKKKNRQATSEVDAVPTGMMNFRKKTLGGK 196
                |:.|...::|.|..:|....:|....| ....:..:....|.|..|..|.........|..
Human   230 GAVPKRKDPLTHTSNSLPRSKTVMKTGSAGL-SGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTP 293

  Fly   197 RTVSSNFVSPVAQNDNSTSSRSSSIPPALAHLDSKMVDHILGESMHDFKPVAWEDIAGLESAKST 261
            :|..:|     ..:..:|::|.........::||.:.:.|:.|.:.:...|.::||||.:.||..
Human   294 KTNRTN-----KPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQA 353

  Fly   262 FLEAIIMPLRRPDLFTGVRCPPRGVLLFGPPGTGKTLIAKSIASQAKAKFFSINPSSLTSKWVGD 326
            ..|.:|:|..||:||||:|.|.||:|||||||.|||::||::|:::.|.||:|:.:|||||:||:
Human   354 LQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGE 418

  Fly   327 AEKLVKTLFAVAAAHQPAIIFIDEVDSLLSKRSANENESTLRLKNEFLIHLDGAASNEEIRVLVI 391
            .||||:.|||||...||:|||||||||||.:|...|::::.|||.||||..||..|..:.||||:
Human   419 GEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVM 483

  Fly   392 GATNRPQELDEAVRRRFVRRLYVPLPTREARQKIIEKLIHQVKHNLDVRQVIELAELTDGYSGAD 456
            |||||||||||||.|||::|:||.||..|.|..:::.|:.:....|..:::.:||.:||||||:|
Human   484 GATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSD 548

  Fly   457 VDTLCRYASMAPLRSLTPDQMEVIETHQLPAVTMDDFKQALRVISKSVSSEDCKQFEAWNEIYG 520
            :..|.:.|::.|:|.|.|:|::.:...::..:.:.||.::|:.|.:|||.:..:.:..||:.:|
Human   549 LTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFG 612

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Fign	NP_001259995.1	AAA	282..418	CDD:214640	85/135 (63%)
		AAA	286..416	CDD:278434	81/129 (63%)
		Vps4_C	<480..520	CDD:286426	9/39 (23%)
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269\|PubMed:16602018	1..300		28/140 (20%)
		Required for midbody localization. /evidence=ECO:0000269\|PubMed:18997780	1..194		6/28 (21%)
		Required for interaction with ATL1. /evidence=ECO:0000269\|PubMed:16339213, ECO:0000269\|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269\|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269\|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269\|PubMed:18997780	112..196		7/30 (23%)
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:15269182	114..200		8/34 (24%)
		MIT_spastin	116..195	CDD:239142	7/29 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	224..266		7/42 (17%)
		Sufficient for microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	228..616		153/391 (39%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	270..328		11/62 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	278..312		7/38 (18%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	309..312		1/2 (50%)
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	98/162 (60%)
		AAA_lid_3	533..587	CDD:407720	18/53 (34%)
		Vps4_C	<580..612	CDD:401324	9/31 (29%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1176820at2759
OrthoFinder	1	1.000	-	-		FOG0001088
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100722
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	4	3.820