Protein Alignment Fign and Spast

DIOPT Version :10

• Sequence 1: NP_608763.2
• Gene: Fign / 33544
• FlyBaseID: FBgn0031519
• Length: 523
• Species: Drosophila melanogaster
• Sequence 2: NP_001156342.1
• Gene: Spast / 50850
• MGIID: 1858896
• Length: 614
• Species: Mus musculus
• Alignment Length: 473
• Identity: 169/473 - (35%)
• Similarity: 262/473 - (55%)
• Gaps: 72/473 - (15%)

  Fly    89 YESCQQTE--------MASSDLQAPALEVSSTYPVQQAVKSRPEGQFPESRNNSTKKIDAQQYSS 145
            ||..::.:        ||...||.    :....||.|..||:.: .:.||.|.:.:       :.
Mouse   169 YERARRLQAKMMTNLVMAKDRLQL----LEKLQPVLQFSKSQTD-VYNESTNLTCR-------NG 221

  Fly   146 ESSSQSGFGFRTAREQLIMDELKKKNRQATSEVDAVPTGMMNFRKKTL---------GGKRTVSS 201
            ...|:||             .:.|:....|...:::|      |.||:         |..|..|.
Mouse   222 HLQSESG-------------AVPKRKDPLTHASNSLP------RSKTVLKSGSAGLSGHHRAPSC 267

  Fly   202 NFVS----------PVAQNDNSTSSRSSSIPPA--------------LAHLDSKMVDHILGESMH 242
            :.:|          |.|.....|...:.:..|:              ..::||.:.:.|:.|.:.
Mouse   268 SGLSMVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVD 332

  Fly   243 DFKPVAWEDIAGLESAKSTFLEAIIMPLRRPDLFTGVRCPPRGVLLFGPPGTGKTLIAKSIASQA 307
            :...|.::||||.|.||....|.:|:|..||:||||:|.|.||:|||||||.|||::||::|:::
Mouse   333 NGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAES 397

  Fly   308 KAKFFSINPSSLTSKWVGDAEKLVKTLFAVAAAHQPAIIFIDEVDSLLSKRSANENESTLRLKNE 372
            .|.||:|:.:|||||:||:.||||:.|||||...||:|||||||||||.:|...|::::.|||.|
Mouse   398 NATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTE 462

  Fly   373 FLIHLDGAASNEEIRVLVIGATNRPQELDEAVRRRFVRRLYVPLPTREARQKIIEKLIHQVKHNL 437
            |||..||..|..:.||||:|||||||||||||.|||::|:||.||..|.|..:::.|:.:....|
Mouse   463 FLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPL 527

  Fly   438 DVRQVIELAELTDGYSGADVDTLCRYASMAPLRSLTPDQMEVIETHQLPAVTMDDFKQALRVISK 502
            ..:::.:||.:||||||:|:..|.:.|::.|:|.|.|:|::.:...::..:.:.||.::|:.|.:
Mouse   528 TQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKR 592

  Fly   503 SVSSEDCKQFEAWNEIYG 520
            |||.:..:.:..||:.:|
Mouse   593 SVSPQTLEAYIRWNKDFG 610

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Fign	NP_608763.2	PH-like	<4..50	CDD:473070
		P-loop containing Nucleoside Triphosphate Hydrolases	229..414	CDD:476819	104/184 (57%)
		AAA_lid_3	444..495	CDD:465537	18/50 (36%)
		Vps4_C	<480..520	CDD:462762	9/39 (23%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..298		30/159 (19%)
		Required for midbody localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..192		5/22 (23%)
		Required for interaction with ATL1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..78
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..48
		Required for nuclear localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..48
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	48..85
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	57..65
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	91..112
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250|UniProtKB:Q9UBP0	110..194		6/28 (21%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	112..198		6/32 (19%)
		MIT_spastin	114..193	CDD:239142	6/27 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	220..306		17/104 (16%)
		Sufficient for microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	226..614		154/404 (38%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	268..326		7/57 (12%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	307..310		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	308..310		0/1 (0%)
		RecA-like_spastin	341..504	CDD:410932	99/162 (61%)
		AAA_lid_3	531..585	CDD:465537	18/53 (34%)
		Vps4_C	<578..610	CDD:462762	9/31 (29%)