Protein Alignment Mad and smad3a

DIOPT Version :10

• Sequence 1: NP_001259992.1
• Gene: Mad / 33529
• FlyBaseID: FBgn0011648
• Length: 525
• Species: Drosophila melanogaster
• Sequence 2: NP_571646.1
• Gene: smad3a / 58092
• ZFINID: ZDB-GENE-000509-3
• Length: 425
• Species: Danio rerio
• Alignment Length: 466
• Identity: 281/466 - (60%)
• Similarity: 333/466 - (71%)
• Gaps: 71/466 - (15%)

  Fly    89 SLFSFTSPAVKKLLGWKQGD---EEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPGQPSKCVT 150
            |:..||.|.||:|||||:|:   :||||.||||.|||||||| .|.::|||:|::.....:||:|
Zfish     2 SILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKK-TGQLDELEKAITTQDVNTKCIT 65

  Fly   151 IPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVE 215
            ||||||||||||||||||||||||:||||||||||||:.:|||::.|..|:.|||:|||||:|||
Zfish    66 IPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLQSHHELRAIELCEFAFHMKKDEVCVNPYHYQRVE 130

  Fly   216 SPVLPPVLVPRH----SEFAPGHSMLQFNHVAEPSMPHNVSYSNSGFNSHSLSTSNTSVGSPSSV 276
            :|||||||||||    |||.|   :..::|    |:|.|..:                   |:.:
Zfish   131 TPVLPPVLVPRHTEIPSEFPP---LDDYSH----SIPENTIF-------------------PAGI 169

  Fly   277 NSNPNSPYDSLAGTPPPAYSPSEDGNSNNPNDGGQLLDAQMG----------------------D 319
              .|.|.|  :..||||.|. ||||.::         |.||.                      |
Zfish   170 --EPQSNY--IPETPPPGYI-SEDGETS---------DHQMNRSMDTGSPNLSPNPVSPAHNNLD 220

  Fly   320 VAQVSYSEPAFWASIAYYELNCRVGEVFHCNNNSVIVDGFTNPSNNSDRCCLGQLSNVNRNSTIE 384
            :..|:|.|||||.||:|||||.||||.||.:..|:.|||||:|| ||:|.|||.|||||||:.:|
Zfish   221 LQPVTYCEPAFWCSISYYELNQRVGETFHASQPSLTVDGFTDPS-NSERFCLGLLSNVNRNAAVE 284

  Fly   385 NTRRHIGKGVHLYYVTGEVYAECLSDSAIFVQSRNCNYHHGFHPSTVCKIPPGCSLKIFNNQEFA 449
            .||||||:||.|||:.|||:|||||||||||||.|||..:|:||:|||||||||:||||||||||
Zfish   285 LTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFA 349

  Fly   450 QLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQM 514
            .||:||||.||||||:||:||||||||||||||||.||.|||||||||:||:|||||||||||||
Zfish   350 ALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQM 414

  Fly   515 GSPHNAISSVS 525
            |||....||||
Zfish   415 GSPSIRCSSVS 425

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	86/125 (69%)
		MH2_SMAD_1_5_9	325..525	CDD:199822	154/199 (77%)
smad3a	NP_571646.1	MH1_SMAD_2_3	7..131	CDD:199815	85/124 (69%)
		MH2_SMAD_2_3	224..414	CDD:199826	148/190 (78%)