Protein Alignment Mad and SMAD4

DIOPT Version :10

• Sequence 1: NP_001259992.1
• Gene: Mad / 33529
• FlyBaseID: FBgn0011648
• Length: 525
• Species: Drosophila melanogaster
• Sequence 2: NP_005350.1
• Gene: SMAD4 / 4089
• HGNCID: 6770
• Length: 552
• Species: Homo sapiens
• Alignment Length: 528
• Identity: 187/528 - (35%)
• Similarity: 260/528 - (49%)
• Gaps: 115/528 - (21%)

  Fly    98 VKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPG-QPSKCVTIPRSLDGRLQV 161
            |..|:..:||.|.|.:|::|::|||||||::|..::.|..|::..| .|||||||.|:|||||||
Human    20 VHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQV 84

  Fly   162 SHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPVLPPVLVPR 226
            :.|||.|||||.|:||||||.. :|||.::.|||.|..|...||:|||||:||.||.:....:..
Human    85 AGRKGFPHVIYARLWRWPDLHK-NELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTL 148

  Fly   227 HSEFAPGHSMLQFNHV----AEPSMP---HNV--------------SYSNSGF----NSHSLSTS 266
            .|. ||...|::..:|    .:||:.   |::              :||....    .|::.||:
Human   149 QSN-APSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTA 212

  Fly   267 N------TSVGSPSSV--------------NSNPNSPYDSLAGTP---------------PPAYS 296
            |      .|...|:|:              ...|....:...|.|               ...|:
Human   213 NFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYT 277

  Fly   297 PSEDGNSNN---------PNDGGQL-LDAQMGDVAQVS-YSEPAFWASIAYYELNCRVGEVFHCN 350
            |:...:.|.         |:.|... :..::.....:| :..|.:|.||||:|::.:|||.|...
Human   278 PNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVP 342

  Fly   351 NNS--VIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIGKGVHLYYV-TGEVYAECLSDSA 412
            ::.  |.|||:.:|| ..||.||||||||:|...||..|.||||||.|... .|:|:..||||.|
Human   343 SSCPIVTVDGYVDPS-GGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHA 406

  Fly   413 IFVQSRNCNYHHGFHP-STVCKIPPGCSLKIFNNQE----------------------------- 447
            :||||...:...|..| ..|.||.|...:|:|:.::
Human   407 VFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPG 471

  Fly   448 ------FAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQW 506
                  .|..:|.|...|. .|.:|.::|.:|||||||||.:|.||.:..||||||||||..||.
Human   472 PGSVGGIAPAISLSAAAGI-GVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQL 535

  Fly   507 LDKVLTQM 514
            ||:||..|
Human   536 LDEVLHTM 543

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	66/118 (56%)
		MH2_SMAD_1_5_9	325..525	CDD:199822	93/229 (41%)
SMAD4	NP_005350.1	Mediates interaction with ZBTB7A. /evidence=ECO:0000269|PubMed:25514493	1..322		95/303 (31%)
		MH1_SMAD_4	14..138	CDD:199816	66/118 (56%)
		Required for interaction with TSC22D1. /evidence=ECO:0000269|PubMed:15881652	44..69		9/24 (38%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	168..194		3/25 (12%)
		Pneumo_att_G	<176..>281	CDD:114270	15/104 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	236..256		1/19 (5%)
		SAD	275..320		6/44 (14%)
		MH2_SMAD_4	320..541	CDD:199823	91/222 (41%)