Protein Alignment Mad and SMAD3

DIOPT Version :10

• Sequence 1: NP_001259992.1
• Gene: Mad / 33529
• FlyBaseID: FBgn0011648
• Length: 525
• Species: Drosophila melanogaster
• Sequence 2: NP_001393940.1
• Gene: SMAD3 / 4088
• HGNCID: 6769
• Length: 462
• Species: Homo sapiens
• Alignment Length: 496
• Identity: 280/496 - (56%)
• Similarity: 335/496 - (67%)
• Gaps: 91/496 - (18%)

  Fly    87 LGSLFSFTSPAVKKLLGWKQGD---EEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPGQPSKC 148
            :.|:..||.|.||:|||||:|:   :||||.||||.|||||||| .|.::|||:|::.....:||
Human     1 MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKK-TGQLDELEKAITTQNVNTKC 64

  Fly   149 VTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKR 213
            :|||||||||||||||||||||||||:||||||.|||||:.:|||::.|:.|:.|||:|||||:|
Human    65 ITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQR 129

  Fly   214 VESPVLPPVLVPRH----SEFAPGHSMLQFNHVAEPSMPHNVSYSNSGFNSHSLSTSNTSVGSPS 274
            ||:|||||||||||    :||.|   :..::|    |:|.|.::                   |:
Human   130 VETPVLPPVLVPRHTEIPAEFPP---LDDYSH----SIPENTNF-------------------PA 168

  Fly   275 SVNSNPNSPYDSLAGTPPPAYSPSEDGNS-----NNPNDGG--QLLDAQMG------DVAQVSYS 326
            .:....|.|     .||||.|. ||||.:     |:..|.|  .|....|.      |:..|:|.
Human   169 GIEPQSNIP-----ETPPPGYL-SEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYC 227

  Fly   327 EPAFWASIAYYELNCRVGEVFHCNNNSVIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIG 391
            |||||.||:|||||.||||.||.:..|:.|||||:|| ||:|.|||.|||||||:.:|.||||||
Human   228 EPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPS-NSERFCLGLLSNVNRNAAVELTRRHIG 291

  Fly   392 KGVHLYYVTGEVYAECLSDSAIFVQSRNCNYHHGFHPSTVCKIPP-------------------- 436
            :||.|||:.|||:|||||||||||||.|||..:|:||:|||||||
Human   292 RGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGKAPLSTPSPYFWKPSLQTS 356

  Fly   437 -----------------GCSLKIFNNQEFAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEY 484
                             ||:||||||||||.||:||||.||||||:||:||||||||||||||||
Human   357 PCSGSRSLWASLPSPLAGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEY 421

  Fly   485 HRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNAISSVS 525
            .||.|||||||||:||:||||||||||||||||....||||
Human   422 RRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS 462

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	85/125 (68%)
		MH2_SMAD_1_5_9	325..525	CDD:199822	154/236 (65%)
SMAD3	NP_001393940.1	MH1_SMAD_2_3	8..132	CDD:199815	84/124 (68%)
		MH2	224..451	CDD:469593	148/227 (65%)