Protein Alignment Mad and smad2

DIOPT Version :10

• Sequence 1: NP_001259992.1
• Gene: Mad / 33529
• FlyBaseID: FBgn0011648
• Length: 525
• Species: Drosophila melanogaster
• Sequence 2: NP_571441.3
• Gene: smad2 / 30639
• ZFINID: ZDB-GENE-990603-7
• Length: 468
• Species: Danio rerio
• Alignment Length: 497
• Identity: 282/497 - (56%)
• Similarity: 332/497 - (66%)
• Gaps: 87/497 - (17%)

  Fly    87 LGSLFSFTSPAVKKLLGWK--------------QGDEEEKWAEKAVDSLVKKLKKRKGAIEELER 137
            :.|:..||.|.||:|||||              |..:||||.||||.|||||||| .|.::|||:
Zfish     1 MSSILPFTPPVVKRLLGWKKSASGSSGAGGGGEQNGQEEKWCEKAVKSLVKKLKK-TGQLDELEK 64

  Fly   138 ALSCPGQPSKCVTIP------------------------------RSLDGRLQVSHRKGLPHVIY 172
            |::...:.:||||||                              ||||||||||||||||||||
Zfish    65 AITTQNRNTKCVTIPSNCSEIWGLSTPNTIEQWDTSGLYSYPDQTRSLDGRLQVSHRKGLPHVIY 129

  Fly   173 CRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPVLPPVLVPRHSEFAPGHSML 237
            ||:||||||.|||||:.:|.|:|.|:.|:.|||:|||||:|||:|||||||||||:|..
Zfish   130 CRLWRWPDLHSHHELRAIETCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIL------ 188

  Fly   238 QFNHVAEPSMPHNVSYSNSGFNSHSLSTSNTSVGSPSSVNSNPNSPYDSLAGTPPPAYSPSEDGN 302
                   ..:|....|:||       ...||:.  |:.:    ..|.:.:..||||.|. ||||.
Zfish   189 -------TELPPLDDYTNS-------IPENTNF--PTGI----EPPNNYIPETPPPGYI-SEDGE 232

  Fly   303 S-----NNPNDGGQ--------LLDAQMG-DVAQVSYSEPAFWASIAYYELNCRVGEVFHCNNNS 353
            :     |...|.|.        |.....| |:..|:|||||||.||||||||.||||.||.:..|
Zfish   233 ASDQQMNQSMDTGSPAELSPSTLSPVNHGMDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPS 297

  Fly   354 VIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIGKGVHLYYVTGEVYAECLSDSAIFVQSR 418
            :.|||||:|| ||:|.|||.|||||||:|:|.||||||:||.|||:.|||:|||||||||||||.
Zfish   298 LTVDGFTDPS-NSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSP 361

  Fly   419 NCNYHHGFHPSTVCKIPPGCSLKIFNNQEFAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAE 483
            |||..:|:||:|||||||||:||||||||||.||:||||.||||||:||:|||||||||||||||
Zfish   362 NCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAE 426

  Fly   484 YHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNAISSVS 525
            |.||.|||||||||:||:||||||||||||||||....||:|
Zfish   427 YRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS 468

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	86/166 (52%)
		MH2_SMAD_1_5_9	325..525	CDD:199822	157/199 (79%)
smad2	NP_571441.3	MH1_SMAD_2_3	8..173	CDD:199815	85/165 (52%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..254		9/30 (30%)
		MH2_SMAD_2_3	267..457	CDD:199826	151/190 (79%)