DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mad and Smad3

DIOPT Version :9

Sequence 1:	NP_001259992.1	Gene:	Mad / 33529	FlyBaseID:	FBgn0011648	Length:	525	Species:	Drosophila melanogaster
Sequence 2:	NP_037227.1	Gene:	Smad3 / 25631	RGDID:	3032	Length:	425	Species:	Rattus norvegicus

Alignment Length:	459	Identity:	280/459 - (61%)
Similarity:	335/459 - (72%)	Gaps:	54/459 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    87 LGSLFSFTSPAVKKLLGWKQGD---EEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPGQPSKC 148
            :.|:..||.|.||:|||||:|:   :||||.||||.|||||||| .|.::|||:|::.....:||
  Rat     1 MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKK-TGQLDELEKAITTQNVNTKC 64

  Fly   149 VTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKR 213
            :|||||||||||||||||||||||||:||||||.|||||:.:|||::.|:.|:.|||:|||||:|
  Rat    65 ITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQR 129

  Fly   214 VESPVLPPVLVPRH----SEFAPGHSMLQFNHVAEPSMPHNVSYSNSGFNSHSLSTSNTSVGSPS 274
            ||:|||||||||||    :||.|   :..::|    |:|.|.::                   |:
  Rat   130 VETPVLPPVLVPRHTEIPAEFPP---LDDYSH----SIPENTNF-------------------PA 168

  Fly   275 SVNSNPNSPYDSLAGTPPPAYSPSEDGNS-----NNPNDGG--QLLDAQMG------DVAQVSYS 326
            .:....|.|     .||||.|. ||||.:     |:..|.|  .|....|.      |:..|:|.
  Rat   169 GIEPQSNIP-----ETPPPGYL-SEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYC 227

  Fly   327 EPAFWASIAYYELNCRVGEVFHCNNNSVIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIG 391
            |||||.||:|||||.||||.||.:..|:.|||||:|| ||:|.|||.|||||||:.:|.||||||
  Rat   228 EPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPS-NSERFCLGLLSNVNRNAAVELTRRHIG 291

  Fly   392 KGVHLYYVTGEVYAECLSDSAIFVQSRNCNYHHGFHPSTVCKIPPGCSLKIFNNQEFAQLLSQSV 456
            :||.|||:.|||:|||||||||||||.|||..:|:||:|||||||||:||||||||||.||:|||
  Rat   292 RGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSV 356

  Fly   457 NNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNAI 521
            |.||||||:||:||||||||||||||||.||.|||||||||:||:||||||||||||||||....
  Rat   357 NQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRC 421

  Fly   522 SSVS 525
            ||||
  Rat   422 SSVS 425

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	85/125 (68%)
Mad	NP_001259992.1	MH2_SMAD_1_5_9	325..525	CDD:199822	154/199 (77%)
Smad3	NP_037227.1	MH1_SMAD_2_3	8..132	CDD:199815	84/124 (68%)
		Linker	137..231		36/125 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	165..208		15/67 (22%)
		MH2_SMAD_2_3	224..414	CDD:199826	148/190 (78%)
		Sufficient for interaction with XPO4. /evidence=ECO:0000250	271..324		40/52 (77%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3701
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D274383at33208
OrthoFinder	1	1.000	-	-		FOG0000630
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X373
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	6	5.820