Protein Alignment Mad and daf-8

DIOPT Version :10

• Sequence 1: NP_001259992.1
• Gene: Mad / 33529
• FlyBaseID: FBgn0011648
• Length: 525
• Species: Drosophila melanogaster
• Sequence 2: NP_492321.1
• Gene: daf-8 / 187612
• WormBaseID: WBGene00000904
• Length: 546
• Species: Caenorhabditis elegans
• Alignment Length: 534
• Identity: 144/534 - (26%)
• Similarity: 224/534 - (41%)
• Gaps: 134/534 - (25%)

  Fly   106 QGDEEEKWAEKAVDSLVKKLKKRKGAIEELERAL--------SCPGQPSKCVTIPRSLDGRLQVS 162
            :||....||.| |..|:.::.|:....|....|:        .||....|.:
 Worm    29 EGDGTIYWALK-VTRLLSRVAKKHQCFEAFYDAVIKGDPKTRCCPAHNEKLI------------- 79

  Fly   163 HRKGLPHVIYC--RVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPV--LPPVL 223
              ......|.|  |.:|:|.::...::|.:..|::.|::..:.||:|||||:.||.|.  :||::
 Worm    80 --GNFGRAIMCVLRAFRFPVIRYESQVKSILTCRHAFNSHSRNVCLNPYHYRWVELPTCQVPPII 142

  Fly   224 VPRHSEF-----------------------------APGHSM-------LQFNHVAEPSMPHNVS 252
            |.:..::                             .|..:|       |...:.||......::
 Worm   143 VNKELDYGEPPIRTEDALDDWNQKDLKEEQVVASWDVPNVTMRGDEIKLLYDRYAAENGDFQYMN 207

  Fly   253 YSNSG-FNSHSLSTSNTSVGS----------------PSSVNSNPNSPYDSLAGTPPPAYSP--- 297
            |.|.| |:....|::..|:||                |....|..:||....|.  |..:.|
 Worm   208 YGNFGSFDGSPCSSTTMSLGSSFASLLQQSNRQDDEEPGYYRSGLSSPASHAAS--PREFIPNGN 270

  Fly   298 --------------------SEDGNS-NNPN------DGGQLLD-----------AQMGDVAQ-- 322
                                ||:.:| ||.|      ||..:..           .::||:::
 Worm   271 DTISLDQEMMDDSEVSDIMHSENFSSENNGNRKPTYADGRPITPIEPRPLYRSSALELGDLSRQA 335

  Fly   323 -----VSYSEPAFWASIAYYELNCRVGEVFHCNNNSVIVDGFTNPSNNSD---RCCLGQLSNVNR 379
                 |.|.|...|..:.|||....:||......:..::||||....:|:   |..||..:|.||
 Worm   336 GIYECVEYEESPSWLKLIYYEEGTMIGEKADVEGHHCLIDGFTASRTDSETRSRFSLGWYNNPNR 400

  Fly   380 NSTIENTRRHIGKGVHLYYVTGEVYAECLSDSAIFVQSRNCNYHHGFHPSTVCKIPPGCSLKIFN 444
            :......|..|||||..|.:.||||.|.|.:..:||||...|..:||..:||.|:||..::|:|:
 Worm   401 SPQTAEVRGLIGKGVRFYLLAGEVYVENLCNIPVFVQSIGANMKNGFQLNTVSKLPPTGTMKVFD 465

  Fly   445 NQEFAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDK 509
            .:.|::.|..:....::.||.|::|||:|:||.||||..|.|..|..:|.|.:.||:.|:.|:|.
 Worm   466 MRLFSKQLRTAAEKTYQDVYCLSRMCTVRVSFCKGWGEHYRRSTVLRSPVWFQAHLNNPMHWVDS 530

  Fly   510 VLTQMGSPHNAISS 523
            |||.||:|....||
 Worm   531 VLTCMGAPPRICSS 544

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	29/119 (24%)
		MH2_SMAD_1_5_9	325..525	CDD:199822	79/202 (39%)
daf-8	NP_492321.1	DWA	32..135	CDD:214708	28/118 (24%)
		DWB	348..522	CDD:197770	65/173 (38%)