Protein Alignment Mad and Smad4

DIOPT Version :9

• Sequence 1: NP_001259992.1
• Gene: Mad / 33529
• FlyBaseID: FBgn0011648
• Length: 525
• Species: Drosophila melanogaster
• Sequence 2: NP_032566.2
• Gene: Smad4 / 17128
• MGIID: 894293
• Length: 551
• Species: Mus musculus
• Alignment Length: 553
• Identity: 194/553 - (35%)
• Similarity: 258/553 - (46%)
• Gaps: 166/553 - (30%)

  Fly    98 VKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPG-QPSKCVTIPRSLDGRLQV 161
            |..|:..:||.|.|.:|::|::|||||||::|..::.|..|::..| .|||||||.|:|||||||
Mouse    20 VHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQV 84

  Fly   162 SHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPVL------- 219
            :.|||.|||||.|:||||||.. :|||.::.|||.|..|...||:|||||:||.||.:
Mouse    85 AGRKGFPHVIYARLWRWPDLHK-NELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTL 148

  Fly   220 ----PPVLVPRHSEF-----------APGHSMLQFNH---------------------------- 241
                |.:||  ..|:           ..|||:....|
Mouse   149 QSNAPSMLV--KDEYVHDFEGQPSLPTEGHSIQTIQHPPSNRASTETYSAPALLAPAESNATSTT 211

  Fly   242 ------VAEPSMPHNV---SYS---------------NSGFNS-----HSLSTSNTSVGSPSSVN 277
                  ||..|.|.::   |:|               .:||.:     |..||: |..||.::..
Mouse   212 NFPNIPVASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTAQPATYHHNSTT-TWTGSRTAPY 275

  Fly   278 SNPNSPYDS---LAGTPPPAYSPSEDGN--------------SNNPNDGGQLLDAQMGDVAQVSY 325
            : ||.|:..   |...||   .|...|:              ||:|
Mouse   276 T-PNLPHHQNGHLQHHPP---MPPHPGHYWPVHNELAFQPPISNHP------------------- 317

  Fly   326 SEPAFWASIAYYELNCRVGEVFHCNNNS--VIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRR 388
             .|.:|.||||:|::.:|||.|...::.  |.|||:.:|| ..||.||||||||:|...||..|.
Mouse   318 -APEYWCSIAYFEMDVQVGETFKVPSSCPVVTVDGYVDPS-GGDRFCLGQLSNVHRTEAIERARL 380

  Fly   389 HIGKGVHLYYV-TGEVYAECLSDSAIFVQSRNCNYHHGFHP-STVCKIPPGCSLKIFNNQE---- 447
            ||||||.|... .|:|:..||||.|:||||...:...|..| ..|.||.|...:|:|:.::
Mouse   381 HIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQ 445

  Fly   448 -------------------------------FAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWG 481
                                           .|..:|.|...|. .|.:|.::|.:|||||||||
Mouse   446 MQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGI-GVDDLRRLCILRMSFVKGWG 509

  Fly   482 AEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQM 514
            .:|.||.:..||||||||||..||.||:||..|
Mouse   510 PDYPRQSIKETPCWIEIHLHRALQLLDEVLHTM 542

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	66/118 (56%)
		MH2_SMAD_1_5_9	325..525	CDD:199822	93/229 (41%)
Smad4	NP_032566.2	Mediates interaction with ZBTB7A. /evidence=ECO:0000250|UniProtKB:Q13485	1..321		102/328 (31%)
		MH1_SMAD_4	14..138	CDD:199816	66/118 (56%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	166..192		4/25 (16%)
		SAD	274..319		11/68 (16%)
		MH2_SMAD_4	319..540	CDD:199823	91/222 (41%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3701
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810