Protein Alignment HTR1B and Octalpha2R

DIOPT Version :10

• Sequence 1: NP_000854.1
• Gene: HTR1B / 3351
• HGNCID: 5287
• Length: 390
• Species: Homo sapiens
• Sequence 2: NP_001262715.1
• Gene: Octalpha2R / 42258
• FlyBaseID: FBgn0038653
• Length: 740
• Species: Drosophila melanogaster
• Alignment Length: 622
• Identity: 139/622 - (22%)
• Similarity: 222/622 - (35%)
• Gaps: 256/622 - (41%)

Human    22 QANLSSAPSQNCSAKDYI-------YQDSISLPWKVLLVMLLALITLATTLSNAFVIATVYRTRK 79
            |::.|.|.|.|.:..::.       |....:|...|:..:::.::.:...:.|..||..:...:.
  Fly    81 QSSASGATSGNLTNPNHTEVHWDGRYPSGYTLTHIVIASIIVTILMIIIVVGNMLVIIAIATEKS 145

Human    80 LHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQVVCDFWLSSDITCCTASILHLCVIA 144
            |....|:.||||||.|..:.:::||.|....:.|.|..|...||...:.|:..|||||::||:|:
  Fly   146 LKNIQNWFIASLAVADFFLGLIIMPFSLANELMGYWIFGSWWCDIHSAMDVLLCTASIMNLCLIS 210

Human   145 LDRYWAITDAVEYSAKRTPKRAAVMIALVWVFSISISLPPFF-WRQAKAEEEVSECVVNTDHILY 208
            |||||:||.||:|...|||.||||||..||:.|..|.:||.. |:....|..:.:|.::.| |.|
  Fly   211 LDRYWSITKAVDYLKSRTPARAAVMITAVWIMSALICIPPLLGWKVKMPEGPLPKCELSED-IGY 274

Human   209 TVYSTVGAFYFPTLLLIALYGRIYVEARSRI---LKQTPNRTGKR-------------------- 250
            .:||.:|:||.|:.:::.:|.|||..|::|.   :|:.|.:|...
  Fly   275 VLYSALGSFYIPSCIMVFVYIRIYFAAKARARRGIKKHPRKTNNEQVTSFTTAKKGTIPMPSSSG 339

Human   251 LTRAQL----------------------------------------------------------- 256
            ::..||
  Fly   340 VSALQLHQQRQIATIETPPNSASLPMQIPTVTMDLASDISTSEAGELEAVAAQTVLAYANPNGAG 404

Human   257 -------------------------------------------ITDSPGSTSSVTSINS------ 272
                                                       :..|.||.||:..:|:
  Fly   405 TNSVTVVSSGNGGPGASGVPPSSPKETLQVSTIATGRVGLNVPVPPSMGSNSSINVLNNNNGTVA 469

Human   273 ------------------------------RVPDV--------------------PSESGSPV-- 285
                                          ||..:                    ||.|.|.|
  Fly   470 MDGGATAAGGGNGSAVATPGGLQQTGGNELRVSPIAGRCRALSVGVDTDMVSEFDPSSSDSGVVS 534

Human   286 ---YVNQVKVRVSDALL------------------------------------------------ 299
               .|..:|.|:...:.
  Fly   535 RCAVVKPLKFRLCQPIFGRKSSNQQRRNEAKAAAKNQQSSGVASKNQQQQQQQQQQQVVKSELEP 599

Human   300 -------------EKKKLMAARERKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHLAIF 351
                         ||:::...:|::||..||:|:|:||.||||||.:.:::|.|...|....:.|
  Fly   600 AIPKTPKPRDPEKEKRRIARKKEKRATLILGLIMGSFIACWLPFFFLYILVPACSSHCNIPESAF 664

Human   352 DFFTWLGYLNSLINPIIYTMSNEDFKQAFHKLIRFKC 388
            ....||||:||.:||.|||:.|:||::||.:::...|
  Fly   665 AVAFWLGYMNSALNPAIYTIFNKDFRRAFRRILFKXC 701

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HTR1B	NP_000854.1	7tmA_5-HT1B_1D	46..380	CDD:320455	130/581 (22%)
		TM helix 1	51..77	CDD:320455	3/25 (12%)
		TM helix 2	84..110	CDD:320455	11/25 (44%)
		TM helix 3	122..152	CDD:320455	16/29 (55%)
		DRY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	146..148		1/1 (100%)
		TM helix 4	164..187	CDD:320455	12/23 (52%)
		TM helix 5	205..234	CDD:320455	12/28 (43%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	259..281		10/77 (13%)
		TM helix 6	307..337	CDD:320455	15/29 (52%)
		TM helix 7	348..373	CDD:320455	12/24 (50%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	365..369		2/3 (67%)
Octalpha2R	NP_001262715.1	7tmA_alpha2_AR	132..>307	CDD:320187	72/175 (41%)
		TM helix 2	150..176	CDD:320187	11/25 (44%)
		TM helix 3	188..218	CDD:320187	16/29 (55%)
		TM helix 4	230..253	CDD:320187	12/22 (55%)
		TM helix 5	271..300	CDD:320187	13/29 (45%)
		7tm_GPCRs	<616..693	CDD:475119	33/76 (43%)
		TM helix 6	626..648	CDD:410628	13/21 (62%)
		TM helix 7	661..686	CDD:410628	12/24 (50%)