DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Duox and FRE3

DIOPT Version :9

Sequence 1:	NP_001259968.1	Gene:	Duox / 33477	FlyBaseID:	FBgn0283531	Length:	1537	Species:	Drosophila melanogaster
Sequence 2:	NP_015026.1	Gene:	FRE3 / 854563	SGDID:	S000005908	Length:	711	Species:	Saccharomyces cerevisiae

Alignment Length:	707	Identity:	143/707 - (20%)
Similarity:	243/707 - (34%)	Gaps:	220/707 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   966 KEYKGDFVAIGLDCKGAKQNFLDTSTNVARMTSFNIEPMQDKPRHWLL--AKWDAYITFLEEN-- 1026
            :.::..|.||..||    ..:.|   |:..||:.:...|.:....:::  ::..|.:|:..|.
Yeast    86 RTFEKTFAAIKEDC----AYYTD---NLQNMTNADFYNMLNNGTTYIIQYSEGSANLTYPIEMDA 143

  Fly  1027 --RQNIFY-----------------LFLFYVVTIVLFVERFIHY-SFMAEHTDL------RHIMG 1065
              |:|.:|                 :...|.|.:::... .:|| |:....|.|      |::..
Yeast   144 QVRENYYYSYHGFYANYDIGHTYGGIICAYFVGVMILAS-ILHYLSYTPFKTALFKQRLVRYVRR 207

  Fly  1066 -VGIAITRGSAASLSFCY-------------------SLLLLTM---------SRNLITKLKEFP 1101
             :.|....|..|| ||.|                   .|:|.|:         ..|||...:...
Yeast   208 YLTIPTIWGKHAS-SFSYLKIFTGFLPTRSEGVIILGYLVLHTVFLAYGYQYDPYNLIFDSRREQ 271

  Fly  1102 IQQYI------------PL-------------------DSHIQFHKIAACTALFFSVLHTVGHIV 1135
            |.:|:            ||                   .|.|.|||.........:|:|  |...
Yeast   272 IARYVADRSGVLAFAHFPLIALFAGRNNFLEFISGVKYTSFIMFHKWLGRMMFLDAVIH--GAAY 334

  Fly  1136 NFYHVSTQSHENLRCLTREVHFASDY--KPDITFWLFQTVTGTTGVMLFIIMCIIFVFAHPTIRK 1198
            ..|.|                |..|:  ..:.|:|.|       ||....|:.::..|:....||
Yeast   335 TSYSV----------------FYKDWAASKEETYWQF-------GVAALCIVGVMVFFSLAMFRK 376

  Fly  1199 KAYNFFWNMHTLYIGLYLLSLIHGLARLTGPPRFWMFFLGPGIVYTLDKIVSL-RTKYMALDVID 1262
            ..|..|..:|.:...|:..:....:..|:|..  |::  ....::|:|:::.: |..|.......
Yeast   377 FFYEAFLFLHIVLGALFFYTCWEHVVELSGIE--WIY--AAIAIWTIDRLIRIVRVSYFGFPKAS 437

  Fly  1263 TDLLPSDVIKIKFYRPPNL-KYLSGQWVRLSCTAFRPH----EMHSFT-LTSAPHENFLSCHIKA 1321
            ..|:..|:|::...||..| |...||:|.:|   |..|    :.|.|| |.|...:..|:..:|.
Yeast   438 LQLVGDDIIRVTVKRPVRLWKAKPGQYVFVS---FLHHLYFWQSHPFTVLDSIIKDGELTIILKE 499

  Fly  1322 QGPWTWKLRNYFDPCNYNPEDQPKIRIEGPFGGGNQDWYKFEVAVMVGGGIGVTPYASILNDLVF 1386
            :...|..::.|.  |....:...::.||||: |.:.....::..:::.||.|:.  ..|.:.:..
Yeast   500 KKGVTKLVKKYV--CCNGGKASMRLAIEGPY-GSSSPVNNYDNVLLLTGGTGLP--GPIAHAIKL 559

  Fly  1387 GTSTNRYSGVACKKVYFLWICPSHKHFEWFIDVLRDVEKKDVTNVLE---------------IHI 1436
            | .|:..:|               |.|...:..:|..      ||||               :||
Yeast   560 G-KTSAATG---------------KQFIKLVIAVRGF------NVLEAYKPELMCLEDLNVQLHI 602

  Fly  1437 F---------------ITQFFHKFD-----LRTTMLYICENHFQRLSKTSIFTGLKAVNHFGRPD 1481
            :               |:|...|.|     :.||:        ::......|.|  .|.|.|||:
Yeast   603 YNTMEVPALTPNDSLEISQQDEKADGKGVVMATTL--------EQSPNPVEFDG--TVFHHGRPN 657

  Fly  1482 MSSFLKFVQKKHSYVSKIGVFSCGPRPLTKSV--MSACDEVNKTRKLPYFIHHFENF 1536
            :...|..|...:   ..:.|..|||......|  .:|...:.|..|.   |.:||.:
Yeast   658 VEKLLHEVGDLN---GSLAVVCCGPPVFVDEVRDQTANLVLEKPAKA---IEYFEEY 708

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Duox	NP_001259968.1	An_peroxidase	70..580	CDD:281139
		dual_peroxidase_like	72..625	CDD:188652
		EFh	827..882	CDD:238008
		EFh	860..921	CDD:238008
		EF-hand_7	860..920	CDD:290234
		EFh	895..966	CDD:238008	143/707 (20%)
		EF-hand_7	899..965	CDD:290234
		Ferric_reduct	1084..1217	CDD:280043	35/174 (20%)
		NOX_Duox_like_FAD_NADP	1265..1536	CDD:99783	70/313 (22%)
FRE3	NP_015026.1	Ferric_reduct	283..396	CDD:396386	27/137 (20%)
FRE3	NP_015026.1	NOX_Duox_like_FAD_NADP	435..709	CDD:99783	70/320 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0039
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R458
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.840