DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Duox and RBOH F

DIOPT Version :9

Sequence 1:	NP_001259968.1	Gene:	Duox / 33477	FlyBaseID:	FBgn0283531	Length:	1537	Species:	Drosophila melanogaster
Sequence 2:	NP_564821.1	Gene:	RBOH F / 842710	AraportID:	AT1G64060	Length:	944	Species:	Arabidopsis thaliana

Alignment Length:	815	Identity:	204/815 - (25%)
Similarity:	375/815 - (46%)	Gaps:	101/815 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly   791 ARAETRERRQKRLEYFFREAYALTFGLRPGERRRR-----SDASSDGEVMTVMRTSLSKAEFAAA 850
            |||..::|.|  |:.....|.....|||....:::     :|..|:.|... ....:.:::||..
plant   159 ARALRKQRAQ--LDRTRSSAQRALRGLRFISNKQKNVDGWNDVQSNFEKFE-KNGYIYRSDFAQC 220

  Fly   851 LGMKPNDMFVRKMFNIVDKDQD---GRISFQEFLETVVLFSRGKTDDKLRIIFDMCDNDRNGVID 912
            :|||.:..|..::|:.:.:.:.   .:|:..|..|.....:....|.:|:|.||:.|.:.:|.|.
plant   221 IGMKDSKEFALELFDALSRRRRLKVEKINHDELYEYWSQINDESFDSRLQIFFDIVDKNEDGRIT 285

  Fly   913 KGELSEMMRSLVEIARTTSLGDDQVTELIDGMFQDVGLEHKNHLTYQDFKLMMKEYKGDFVAIGL 977
            :.|:.|:: .|...|...|...:|..|....:.:::..|...::.....:.::.:
plant   286 EEEVKEII-MLSASANKLSRLKEQAEEYAALIMEELDPERLGYIELWQLETLLLQ---------- 339

  Fly   978 DCKGAKQNFLDTSTNVARMTSFNIEPMQDKPRHWLLAKWDAYITFLEENRQNIFYLFLFYVVTIV 1042
              |....|:....:..::..|.|::.::.|.|...::....||  ::||.:.|:.|.|:.::.|.
plant   340 --KDTYLNYSQALSYTSQALSQNLQGLRGKSRIHRMSSDFVYI--MQENWKRIWVLSLWIMIMIG 400

  Fly  1043 LFVERFIHYSFMAEHTDLRHIMGVGIAITRGSAASLSFCYSLLLLTMSRNLITKLKEFPIQQYIP 1107
            ||:.:|..|    :..|..|:||..:...:|:|.:|.|..:|:|..:.||.||.|:...:..::|
plant   401 LFLWKFFQY----KQKDAFHVMGYCLLTAKGAAETLKFNMALILFPVCRNTITWLRSTRLSYFVP 461

  Fly  1108 LDSHIQFHKIAACTALFFSVLHTVGHIVNFYH--VSTQSHENLRCLTREVHFASDYKPDITFWLF 1170
            .|.:|.|||..|...:...:||...|:...:.  |....::..|.|   .|:....:|.. |.|.
plant   462 FDDNINFHKTIAGAIVVAVILHIGDHLACDFPRIVRATEYDYNRYL---FHYFQTKQPTY-FDLV 522

  Fly  1171 QTVTGTTGVMLFIIMCIIFVFA------------HPTIRKKAYNFFWNMHTLYIGLYLLSLIHGL 1223
            :...|.||:::.|:|.|.|..|            .|..|...:|.||..|.|::.:|:|.::||:
plant   523 KGPEGITGILMVILMIISFTLATRWFRRNLVKLPKPFDRLTGFNAFWYSHHLFVIVYILLILHGI 587

  Fly  1224 ARLTGPPRF----WMFFLGPGIVYTLDKIVS-LRTKYMALDVIDTDLLPSDVIKIKFYRPPNLKY 1283
            ......|.:    ||:...|.::|..::.:. .|:...::.::...:.|.:|:.::..:|...:|
plant   588 FLYFAKPWYVRTTWMYLAVPVLLYGGERTLRYFRSGSYSVRLLKVAIYPGNVLTLQMSKPTQFRY 652

  Fly  1284 LSGQWVRLSCTAFRPHEMHSFTLTSAPHENFLSCHIKAQGPWTWKLRNYFDPCNYNP-------- 1340
            .|||::.:.|.|..|.|.|.|::||||.::::|.||:..|.||.:|:..|......|
plant   653 KSGQYMFVQCPAVSPFEWHPFSITSAPEDDYISIHIRQLGDWTQELKRVFSEVCEPPVGGKSGLL 717

  Fly  1341 -------EDQPKIRIEGPFGGGNQDWYKFEVAVMVGGGIGVTPYASILNDLV------------- 1385
                   :..||:.|:||:|...||:.|::|.::||.|||.||:.|||.||:
plant   718 RADETTKKSLPKLLIDGPYGAPAQDYRKYDVLLLVGLGIGATPFISILKDLLNNIVKMEEHADSI 782

  Fly  1386 --------FGTSTN-----RYSGVACKKVYFLWICPSHKHFEWFIDVLRDVEKKDVTNVLEIHIF 1437
                    :.|.:|     |...:.....||.|:......|:||..|:.:|.:.|...|:|:|.:
plant   783 SDFSRSSEYSTGSNGDTPRRKRILKTTNAYFYWVTREQGSFDWFKGVMNEVAELDQRGVIEMHNY 847

  Fly  1438 ITQFFHKFDLRTTMLYICE--NHFQRLSKTSIFTGLKAVNHFGRPDMSSFLKFVQKKHSYVSKIG 1500
            :|..:.:.|.|:.::.:.:  ||.:  :...|.:|.:...||.||:....|..:..||.. ::||
plant   848 LTSVYEEGDARSALITMVQALNHAK--NGVDIVSGTRVRTHFARPNWKKVLTKLSSKHCN-ARIG 909

  Fly  1501 VFSCGPRPLTKSVMSACDEVNK--TRKLPYFIHHF 1533
            ||.||...|.|.:...|:..|:  :.|..:...||
plant   910 VFYCGVPVLGKELSKLCNTFNQKGSTKFEFHKEHF 944

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Duox	NP_001259968.1	An_peroxidase	70..580	CDD:281139
		dual_peroxidase_like	72..625	CDD:188652
		EFh	827..882	CDD:238008	12/57 (21%)
		EFh	860..921	CDD:238008	14/63 (22%)
		EF-hand_7	860..920	CDD:290234	14/62 (23%)
		EFh	895..966	CDD:238008	15/70 (21%)
		EF-hand_7	899..965	CDD:290234	13/65 (20%)
		Ferric_reduct	1084..1217	CDD:280043	40/146 (27%)
		NOX_Duox_like_FAD_NADP	1265..1536	CDD:99783	90/314 (29%)
RBOH F	NP_564821.1	NADPH_Ox	169..267	CDD:400631	18/98 (18%)
		EFh	<247..294	CDD:415501	13/47 (28%)
		EF-hand_7	266..337	CDD:404394	16/71 (23%)
		Ferric_reduct	435..582	CDD:396386	40/150 (27%)
		FAD_binding_8	624..740	CDD:285293	32/115 (28%)
		NAD_binding_6	746..927	CDD:369657	50/183 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	41	1.000	Domainoid score	I4812
eggNOG	1	0.900	-	-		E1_KOG0039
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000155
OrthoInspector	1	1.000	-	-		otm2409
orthoMCL	1	0.900	-	-		OOG6_100242
Panther	1	1.100	-	-	O	PTHR11972
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X184
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	8	7.810