DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Unc-89 and trio

DIOPT Version :9

Sequence 1:	NP_001097440.1	Gene:	Unc-89 / 3346201	FlyBaseID:	FBgn0053519	Length:	4218	Species:	Drosophila melanogaster
Sequence 2:	NP_651960.2	Gene:	trio / 43974	FlyBaseID:	FBgn0024277	Length:	2263	Species:	Drosophila melanogaster

Alignment Length:	765	Identity:	171/765 - (22%)
Similarity:	277/765 - (36%)	Gaps:	185/765 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    69 NPTMSSSNGKESGDAEF-----------------------RKLTILRELVETEEEFSRDLLHVVE 110
            |.::|||:|....|...                       ||..|:.||::||..:..||...::
  Fly  1243 NSSISSSSGDRHSDPTLEAKLNSSNKENKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIK 1307

  Fly   111 KYIKGIDK-PVVPRSVRDNKDIIFCNFLQIAEFHNNVLKEGLKCYSNQPNMVAKTFLRLERDFDK 174
            .:::.... ..||.::...:|:||.|..:|..||..:....|:.|...|..|...|:.....||.
  Fly  1308 CFLEEFRAGKSVPSALIGQEDVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDM 1372

  Fly   175 HVVYCQNEPLAQDYL---GSSPDAKKYFQELSKQLGDDKSLAEHLKLPIQRINDYQLLFKDFIKY 236
            :|.||:|:|.:.:.|   |.|     :|:||.::|..|..|..:|..|:|||..||||.||.:.
  Fly  1373 YVHYCKNKPTSNNLLVQHGGS-----FFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLLS- 1431

  Fly   237 SLSLKENVKDLERALELMLSVPSRAYDNRFLSSIEGCRGNIYKLGRLLL----HAW-CNVVDKEG 296
              ..:|:..:::..||:||:||.:|.|...||.:|.|..::.|||.::|    .|| ...:.::|
  Fly  1432 --CCEESHGEIKEGLEVMLNVPKKANDAMHLSLLENCDVSVDKLGEVVLQDAFQAWDTKQIIRKG 1494

  Fly   297 KAHDRYCFLFKSRILVTKVRKISENRSVFILQNIVK----------------------------- 332
            :  :|..|||:..:|..|..|.|         |:||
  Fly  1495 R--ERRVFLFELYLLFAKEVKES---------NVVKYQFKSKLMTTDMGITEHIEGDETKFAVWT 1548

  Fly   333 -----LPLCNIELKA---DEKQIHLS--LKAPEANSFLPIDIK-PHGPEAHLTWFNEISSHINQD 386
                 |..|.|.|||   :.|||.:.  .:..:...|....:. |..|..|.......|..:::.
  Fly  1549 GRSPMLSDCRIVLKATSLETKQIWVKKLREVMQETCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQ 1613

  Fly   387 VTLQEHNADDLKVDASQIASESELILHLPQRAEAHDPNLSVRPSDVAENYFLSKETKERLQHEQQ 451
            :|..:|:...|....|...::|             |..|.
  Fly  1614 LTENDHDRCSLASFGSGNTTDS-------------DNKLG------------------------- 1640

  Fly   452 ELLKLEQEA---IELYKKQQSSKSVS-SKTESVEITSSQVKSSSEVRKVVSPPPPPQAQVKE-VT 511
                 .|||   :..|.....|..:| ||.:.|||.........:...|...|....|.|:| :.
  Fly  1641 -----NQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLV 1700

  Fly   512 PVKVVSSPPPPKEITPAKVATPPPQPQVVTSPVKEVAPPPQPRAVASPAK--------------- 561
            ||.|:..||...:......|..........|.:::.:...:..|::|..|
  Fly  1701 PVSVLKPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSGRNWLPLMNR 1765

  Fly   562 ---EVTPSQSEPVKAPSPIKEVRKEVPPSASHSKEVEALVATEIRESLTETRSTVVESGQSSEIR 623
               :..||....||.||     .|.:.....|::|    :|.:..:....:.:||:.|.|.....
  Fly  1766 KGTDKPPSSKPLVKKPS-----EKNLRTPQKHAEE----LAEQQNQQPGASPATVLPSSQFPSST 1821

  Fly   624 EEIVVTEESSLEGKQVVALEREPSPCSIPKIQVYRPVECENPVVTKHKPIELKDIVGYSESLRDG 688
            :..........|..:.|.||..|.         .:|:: |..::....|...||.   .||  .|
  Fly  1822 QHPGGAGAGDYEPDEEVGLELPPP---------MKPIQ-EPHLIANGPPAFAKDA---KES--SG 1871

  Fly   689 DTAPAGGSPGRQQGYSANITDHASLTIWNNRLANIAGDRSGANQHLQQSG 738
            :.|    |||:..|...:..:....|......:|...|..|..::...:|
  Fly  1872 NMA----SPGKMDGNPLSEIEEIVKTTTEQHESNSRVDGGGGVENASTNG 1917

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Unc-89	NP_001097440.1	RhoGEF	90..260	CDD:238091	55/173 (32%)
		PH_unc89	275..388	CDD:270134	32/157 (20%)
		Atrophin-1	<493..690	CDD:331285	42/215 (20%)
		TonB_N	502..>596	CDD:318287	22/112 (20%)
		I-set	1017..1108	CDD:333254
		I-set	1123..1212	CDD:254352
		I-set	<1236..1299	CDD:333254
		I-set	1313..1403	CDD:254352
		Ig_3	1406..1487	CDD:316449
		I-set	1499..1595	CDD:254352
		I-set	1599..1690	CDD:333254
		I-set	1694..1786	CDD:254352
		I-set	<1836..1903	CDD:333254
		I-set	1922..2005	CDD:333254
		I-set	2018..2108	CDD:333254
		I-set	2113..2214	CDD:333254
		I-set	2220..2302	CDD:254352
		I-set	2318..2408	CDD:254352
		I-set	2415..2506	CDD:254352
		I-set	2519..2608	CDD:254352
		I-set	2615..2696	CDD:254352
		I-set	2717..2805	CDD:254352
		FN3	2834..2925	CDD:238020
		I-set	<2996..3063	CDD:333254
		I-set	3067..3157	CDD:333254
		PK_Unc-89_rpt1	3182..3440	CDD:271011
		I-set	3654..3744	CDD:333254
		FN3	3748..3840	CDD:238020
		STKc_Unc-89_rpt2	3893..4151	CDD:271014
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103
		SPEC	782..997	CDD:238103
		SPEC	897..1119	CDD:238103
		SPEC	1125..1226	CDD:197544
		RhoGEF	1287..1456	CDD:279015	56/176 (32%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	29/132 (22%)
		PH	1495..1580	CDD:278594	20/95 (21%)
		SH3_Kalirin_1	1643..1707	CDD:212786	18/63 (29%)
		RhoGEF	1945..2122	CDD:279015
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4240
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	2	1.810