DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Unc-89 and Trio

DIOPT Version :9

Sequence 1:	NP_001097440.1	Gene:	Unc-89 / 3346201	FlyBaseID:	FBgn0053519	Length:	4218	Species:	Drosophila melanogaster
Sequence 2:	NP_001101128.3	Gene:	Trio / 310192	RGDID:	1308360	Length:	3103	Species:	Rattus norvegicus

Alignment Length:	1631	Identity:	352/1631 - (21%)
Similarity:	563/1631 - (34%)	Gaps:	552/1631 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 SRDYQAQS----LATDEISVSRGDLV----ELISSKASEKSRCFVRMFDSGDSPKEGWVPIDILE 67
            |:|.:|.|    ..|...:.|..:||    ||:.||.:.:.|....:.|.|||....:.|.|
  Rat  1885 SQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSD--- 1946

  Fly    68 FNPTMSSSNGKESGDAEFRKLT-------ILRELVETEEEFSRDLLHVVEKYIKGIDKPVVPRSV 125
             |..:|||:..:  :.|.||.:       :|:||||||.::.|||..|||.|:..:.:..||..:
  Rat  1947 -NSLLSSSSPID--EMEERKCSSLKRRHYVLQELVETERDYVRDLGCVVEGYMALMKEDGVPDDM 2008

  Fly   126 RDNKDIIFCNFLQIAEFHNN-VLKEGLKCYSNQPNMVAKTFLRLERDFDKHVVYCQNEPLAQDYL 189
            :....|:|.|..||.::|.: .|.|..||..: |..:...|::.||....::|||||:|.::..:
  Rat  2009 KGKDKIVFGNIHQIYDWHRDFFLGELEKCLED-PEKLGSLFVKHERRLHMYIVYCQNKPKSEHIV 2072

  Fly   190 GSSPDAKKYFQELSKQLGDDKSLAEHLKLPIQRINDYQLLFKDFIKYSLSLKENVKDLERALELM 254
            ....|.  :|::|.::||....|.:.|..|:|||..||||.|||:|||.....:..:||:|:|:|
  Rat  2073 SEYIDT--FFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELEKAVEVM 2135

  Fly   255 LSVPSRAYDNRFLSSIEGCRGNIYKLGRLLLHAWCNVVDKEG----KAHDRYCFLFKSRILVTKV 315
            ..||.|..|...:..::|..|.|...|:|||.....|.|::.    :..:|..|||:..::.::.
  Rat  2136 CIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCKERRVFLFEQIVIFSEP 2200

  Fly   316 --RKISENRSVFILQNIVKLP-LC---NIELKADEKQIHLSLKAPEA-NSFLPIDIKPHGPEAHL 373
              :|...:...|:.:|.:|:. ||   |:|  :|..:..|:.:..:| .:|:   :....|....
  Rat  2201 LDKKKGFSMPGFLFKNSIKVSCLCLEENVE--SDPCKFALTSRTGDAVETFI---LHSSSPSVRQ 2260

  Fly   374 TWFNEISSHINQDVTLQEHNADDLKVDASQIASESELILHLPQRAEAHDPNLSVRPSDVAENYFL 438
            ||.:|    |||                                              :.||
  Rat  2261 TWIHE----INQ----------------------------------------------ILEN--- 2272

  Fly   439 SKETKERLQHEQQELLKLEQEAIELYKKQQSSKSVSSKTESVEITSSQVKSSSEVRKVVSPPPPP 503
                       |:..|......|| |::..|....|....|.........|.:......|....|
  Rat  2273 -----------QRNFLNALTSPIE-YQRNHSGGGGSGSGGSSGGGGGSGGSGASSGGSSSHGSGP 2325

  Fly   504 QAQVKEVTPVKVVSSPPPPKEITPAKVATPPPQPQVVTSPVKEVAPPPQPRAVASPAKEVTPSQS 568
            .:          .||.|......|:::    |||         |...| |..|:|.|.    ||:
  Rat  2326 SS----------CSSGPSSSRSRPSRI----PQP---------VRHHP-PMLVSSAAS----SQA 2362

  Fly   569 EPVK-----APSPIKEVRKEVPPSASHSKEVEALVATEIRESLTETRSTVVESGQSSEIREEIVV 628
            |..|     ||||..       |:.|:|..:||.:....|..|         ||.|
  Rat  2363 EADKMSGMSAPSPSL-------PTPSNSLALEASLGQPSRLPL---------SGDS--------- 2402

  Fly   629 TEESSLEGKQVVALEREPSPCSIPKIQVYRPVECENPVVTKHKPIELKDIVGYSESLRDGDTAPA 693
                  ||.     |||..|  |||::|     .|:|         .|.:...|.:.:||:|..|
  Rat  2403 ------EGH-----ERETEP--IPKMKV-----MESP---------RKALGSTSGTSQDGNTKDA 2440

  Fly   694 GGSPGRQQGYSANITDHASLTIWNNRLANIAGDRSGANQHLQQSGPPPPPIPPNFTRMPGFFQPL 758
            .|:.|                                            |:|...|| ||...||
  Rat  2441 RGNLG--------------------------------------------PLPLGKTR-PGAVSPL 2460

  Fly   759 --PLIAYETTIEILIVK-ARPPSPP--------------PPPPPTIKRVLVHTESLEQKTQNFFE 806
              ||   .||......| |.|||.|              |...|:.......::||:::|..
  Rat  2461 NSPL---STTFPSPFGKEAFPPSSPLQKGGSFWSSIPASPASRPSSFTFPGDSDSLQRQTHR--- 2519

  Fly   807 GIYDAASSDTSLRN-----AKQKIRSIKSTVLKSKDSTNYAQDTVQKAKARDFLHIFTPPVKKRP 866
              :.|.|.||...:     ::|.::|.:|...:|..|:|.:...|        .|.:|
  Rat  2520 --HAAPSKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLV--------THEYT------- 2567

  Fly   867 IYEIVEEPVNIFELEGDYTESIADDFREPSADFEARGQSVGGMDDYYSGYSRASTRRYETKTRD- 930
              .:.|:.:|::  :|:..:.:|.:.:.....|.|........:.:..|:....|.....:..|
  Rat  2568 --AVKEDEINVY--QGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIMENPDG 2628

  Fly   931 -YDRGTSYDSTVERSQYGISSRRDRSSVDKVEARSSLLATGRTESRAASRAESRAESRASYSVAE 994
             :.:.||:.:       .:..|:.....||...|.     |:.|:
  Rat  2629 TFKKSTSWHT-------ALRLRKKSEKKDKDGKRD-----GKLEN-------------------- 2661

  Fly   995 SRAGIRSSSRLQEDRPLRSVDKPVVVKMLKSVQVEPGETAHFEIQFKDQPGLVTWLKDNKPLEDR 1059
               |.|        :|...:...|.||:|..
  Rat  2662 ---GYR--------KPREGLSNKVSVKLLNP---------------------------------- 2681

  Fly  1060 LADRITQTAAPMNSYRLDIKNCSETDAGTYTIRAQSASETTTVSAQLAVGQAPGHDETKTNTEPA 1124
                         :|..|:                                           .|.
  Rat  2682 -------------NYIYDV-------------------------------------------PPE 2690

  Fly  1125 FLVSLKD--AEMIENTLFRFMVKIIGDPKPRVKFYKDEKEILETNDRIQIIRDKDY--LGFYELV 1185
            |::.|.:  .|..|..:||  .::.|.||..:.:...|...|..:|...|    .|  :|...|.
  Rat  2691 FVIPLSEVTCETGETVVFR--CRVCGRPKASITWKGPEHNTLNNDDHYNI----SYSDVGEATLK 2749

  Fly  1186 IADVQKTDAGTYSCKATNKHGEANCEAIATTVEDKNPFGALSGQIL-PAGEKPVFQWKRNGEEFD 1249
            |..|...|.|.|:|.|.|..|.|:..|              |.::| |..:..|..||.|.:.|.
  Rat  2750 IIGVSTEDDGVYTCIAVNDMGSASSSA--------------SLRVLGPGSDGIVVTWKDNFDAFY 2800

  Fly  1250 PE------ERFKVLFGEDED----SLALVFQHVK-------PEDAGIYTCVAQTSTGNISCSAEL 1297
            .|      .||.|:...|:.    ::|..|.:.|       ..:.||..        |:.....:
  Rat  2801 SEVAELGRGRFAVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQ--------NLQHPLFV 2857

  Fly  1298 SVQGAIQTLNREPEKPTLVIE------------------HREANASIGGSAILELQCKGFPKPAV 1344
            |:....:|    |....||:|                  ..:..|.:|  .:||         ||
  Rat  2858 SLLDTFET----PTSYVLVLELADQGRLLDCVVRWGSLTEGKVRAHLG--EVLE---------AV 2907

  Fly  1345 QWKHDGEVIQVDDRHKFMYEDEESMSLVIKNVDTVDA----GVYTIEAINELGQDESSINLVVKA 1405
            ::.|:..:..:|.:.:.:..|:......||..|..||    ..|.|..:  ||..|.:...::..
  Rat  2908 RYLHNCRIAHLDLKPENILVDQSLAKPTIKLTDFGDAVQLNTTYYIHQL--LGNPEFAAPEIILG 2970

  Fly  1406 PP--------KIKKITDITCSAGETIKMEIEVE-----------GFPQPTVQ-VTNNGKD----V 1446
            .|        .:..:|.:..| |.:..::..||           .||:...| |:...|:    :
  Rat  2971 NPVSLTADTWSVGVLTYVLLS-GVSPFLDDSVEETCLNICRLDFSFPEDYFQGVSQKAKEFVCFL 3034

  Fly  1447 TAESNVKISSSSIGKSLEKVVVEVKEIKLSQAGNYSIKATNDLSQTSEYWSCTVKSK------PV 1505
            ..|...|..|:::  :|::        :..||||.|.|....| .||...|...:.|      |:
  Rat  3035 LQEDPAKRPSAAL--ALQE--------QWLQAGNGSGKGMGVL-DTSRLTSFIERRKHQNDVRPI 3088

  Fly  1506 -IVKNF 1510
             .:|||
  Rat  3089 RSIKNF 3094

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Unc-89	NP_001097440.1	RhoGEF	90..260	CDD:238091	64/170 (38%)
		PH_unc89	275..388	CDD:270134	31/123 (25%)
		Atrophin-1	<493..690	CDD:331285	49/201 (24%)
		TonB_N	502..>596	CDD:318287	25/98 (26%)
		I-set	1017..1108	CDD:333254	6/90 (7%)
		I-set	1123..1212	CDD:254352	27/92 (29%)
		I-set	<1236..1299	CDD:333254	16/79 (20%)
		I-set	1313..1403	CDD:254352	22/111 (20%)
		Ig_3	1406..1487	CDD:316449	21/104 (20%)
		I-set	1499..1595	CDD:254352	5/19 (26%)
		I-set	1599..1690	CDD:333254
		I-set	1694..1786	CDD:254352
		I-set	<1836..1903	CDD:333254
		I-set	1922..2005	CDD:333254
		I-set	2018..2108	CDD:333254
		I-set	2113..2214	CDD:333254
		I-set	2220..2302	CDD:254352
		I-set	2318..2408	CDD:254352
		I-set	2415..2506	CDD:254352
		I-set	2519..2608	CDD:254352
		I-set	2615..2696	CDD:254352
		I-set	2717..2805	CDD:254352
		FN3	2834..2925	CDD:238020
		I-set	<2996..3063	CDD:333254
		I-set	3067..3157	CDD:333254
		PK_Unc-89_rpt1	3182..3440	CDD:271011
		I-set	3654..3744	CDD:333254
		FN3	3748..3840	CDD:238020
		STKc_Unc-89_rpt2	3893..4151	CDD:271014
Trio	NP_001101128.3	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..27
		SEC14	69..204	CDD:214706
		SPEC	227..445	CDD:238103
		Spectrin 1	311..418
		SPEC	341..566	CDD:238103
		Spectrin 2	538..644
		SPEC	567..783	CDD:238103
		SPEC	787..1014	CDD:238103
		Spectrin 3	878..984
		SPEC	908..1137	CDD:238103
		Spectrin 4	1109..1216
		SPEC	1143..1243	CDD:197544
		RhoGEF	1295..1465	CDD:238091
		PH1_Kalirin_Trio_like	1472..1594	CDD:270060
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1601..1651
		SH3_Kalirin_1	1659..1718	CDD:212786
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1738..1907		6/21 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1927..1956		10/32 (31%)
		RhoGEF	1970..2143	CDD:238091	65/175 (37%)
		PH2_Kalirin_Trio_p63RhoGEF	2148..2286	CDD:270061	38/207 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2287..2556		89/401 (22%)
		SH3_Kalirin_2	2559..2617	CDD:212787	10/76 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2643..2667		8/59 (14%)
		IG	2696..2780	CDD:214652	26/103 (25%)
		STKc_Trio_C	2792..3054	CDD:271015	55/297 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910