DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Unc-89 and Mcf2l

DIOPT Version :9

Sequence 1:	NP_001097440.1	Gene:	Unc-89 / 3346201	FlyBaseID:	FBgn0053519	Length:	4218	Species:	Drosophila melanogaster
Sequence 2:	XP_006253478.1	Gene:	Mcf2l / 117020	RGDID:	619782	Length:	1280	Species:	Rattus norvegicus

Alignment Length:	730	Identity:	169/730 - (23%)
Similarity:	257/730 - (35%)	Gaps:	200/730 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    56 PKEGWVPIDILEFNPTMSSSNGKESGDAEFRKLTILRELVETEEEFSRDLLHVVEKYIKGIDKP- 119
            |::|            .:||.|:|.......:..::.||::||..:..:||.|:|.|...:|.|
  Rat   683 PRQG------------RTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPL 735

  Fly   120 ---VVPRSVRDNKDIIFCNFLQIAEFHNNVLKEGLKCYSNQPNMVAKTFLRLERDFDKHVVYCQN 181
               ::...:::.|:|:|.|..:|..|||.:....|:...:.|.:|.:.||....:|..:..||||
  Rat   736 MAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQN 800

  Fly   182 EPLAQDYLGSSPDAKKYFQELSKQLGDDKSLAEHLKLPIQRINDYQLLFKDFIKYSLSLKENVKD 246
            :|.::.......|. .:|||..|:|....||..:|..|:|||..||||.|:.:|||... |..:|
  Rat   801 KPRSESLWRQCSDC-PFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHC-EGAED 863

  Fly   247 LERALELMLSVPSRAYDNRFLSSIEGCRGNIYKLGRLLLHAWCNVVDKEGKAH------------ 299
            |:.||..:|.:.....|:..|.:|.|..||:..||:||:....:|.....|.|
  Rat   864 LQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPM 928

  Fly   300 DRYCFLFKSRILVTKVRKISEN------------------RSVFILQNIVKLPLCNIELKADEKQ 346
            .|:.||.:..:|..|.|:  ||                  .:|.|.:|:          |.|.|:
  Rat   929 QRHLFLHEKAVLFCKKRE--ENGEGYEKAPSYSYKQSLNMTAVGITENV----------KGDTKK 981

  Fly   347 IHLSLKAPEANSFLPIDIKPHGPEAHLTWFNEISSHINQDVTLQEHNADDLKVDASQIASESELI 411
            ..:...|.|....    |:...||....|.|||.                 ||..||:.:..|..
  Rat   982 FEIWYNAREEVYI----IQAPTPEIKAAWVNEIR-----------------KVLTSQLQACREAS 1025

  Fly   412 LHLPQRA--EAHDPNLSVRPSDVAENYFLSKETKERLQHEQQELLKLEQEAIELYKKQQSSKSVS 474
            .|   ||  ::|...|.. |:..:.....::..| :|:..:.:.|.||             ..||
  Rat  1026 QH---RALEQSHSLPLPT-PASTSPTKGSTRNVK-KLEDRKTDPLCLE-------------GCVS 1072

  Fly   475 SKTESVEITSSQVKSSSEVRKVVSPPPPPQAQVKEVTPVKV-VSSPPPPKEITPAKVATPPPQPQ 538
            |                      |.|.||:....:..|... .||....|..|...:|....|..
  Rat  1073 S----------------------SLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKA 1115

  Fly   539 VVTSPVK-----EVAPPPQPRAVASPAKEVTPSQSEPVKAPSPIKEVRKEVPPSASHSKEVEALV 598
            ..|||.|     ||...|.|..:...:|                          .|||.|
  Rat  1116 SPTSPDKKAKRHEVKSDPTPFGLRGWSK--------------------------TSHSLE----- 1149

  Fly   599 ATEIRESLTETRSTVVESGQSSEIREEIVVTEESSLEGKQVVALEREPSPCSIPKIQVYRPVECE 663
            |.|.....:.....:    .||:..|:..|.....:.||..|.::.|.
  Rat  1150 APEEDGGWSSAEELI----NSSDAEEDGGVGPRKLVPGKYTVLMDGEK----------------- 1193

  Fly   664 NPVVTKHKPIELKDIVGYSESL--RDGDTAPAGGSPGRQQGYSANITDHASLTIWNNRLANIAGD 726
                            |.|::|  |.||.............|..::|......:..:.||.:.|.
  Rat  1194 ----------------GGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGK 1242

  Fly   727 RSGANQHLQQSGPPP 741
            .|.| |.|..|...|
  Rat  1243 SSSA-QCLSSSDSSP 1256

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Unc-89	NP_001097440.1	RhoGEF	90..260	CDD:238091	57/173 (33%)
		PH_unc89	275..388	CDD:270134	32/142 (23%)
		Atrophin-1	<493..690	CDD:331285	39/204 (19%)
		TonB_N	502..>596	CDD:318287	22/99 (22%)
		I-set	1017..1108	CDD:333254
		I-set	1123..1212	CDD:254352
		I-set	<1236..1299	CDD:333254
		I-set	1313..1403	CDD:254352
		Ig_3	1406..1487	CDD:316449
		I-set	1499..1595	CDD:254352
		I-set	1599..1690	CDD:333254
		I-set	1694..1786	CDD:254352
		I-set	<1836..1903	CDD:333254
		I-set	1922..2005	CDD:333254
		I-set	2018..2108	CDD:333254
		I-set	2113..2214	CDD:333254
		I-set	2220..2302	CDD:254352
		I-set	2318..2408	CDD:254352
		I-set	2415..2506	CDD:254352
		I-set	2519..2608	CDD:254352
		I-set	2615..2696	CDD:254352
		I-set	2717..2805	CDD:254352
		FN3	2834..2925	CDD:238020
		I-set	<2996..3063	CDD:333254
		I-set	3067..3157	CDD:333254
		PK_Unc-89_rpt1	3182..3440	CDD:271011
		I-set	3654..3744	CDD:333254
		FN3	3748..3840	CDD:238020
		STKc_Unc-89_rpt2	3893..4151	CDD:271014
Mcf2l	XP_006253478.1	SEC14	145..290	CDD:214706
		SPEC	421..623	CDD:238103
		RhoGEF	702..879	CDD:238091	57/178 (32%)
		PH_Dbs	886..1017	CDD:269934	36/163 (22%)
		PH	900..1014	CDD:278594	29/146 (20%)
		SH3_DBS	1184..1236	CDD:212791	11/84 (13%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4240
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810