DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Unc-89 and Mylk

DIOPT Version :9

Sequence 1:	NP_001097440.1	Gene:	Unc-89 / 3346201	FlyBaseID:	FBgn0053519	Length:	4218	Species:	Drosophila melanogaster
Sequence 2:	NP_647461.3	Gene:	Mylk / 107589	MGIID:	894806	Length:	1950	Species:	Mus musculus

Alignment Length:	2803	Identity:	570/2803 - (20%)
Similarity:	922/2803 - (32%)	Gaps:	1032/2803 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly  1109 GQAPGH--------------------DETKTN----TE-PAFLVSLKDAEMIENTLFRFMVKIIG 1148
            ||.||.                    |.:|.:    || |||::..::..:.|....:|..::.|
Mouse     3 GQLPGKTMGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRNLCVKEGATAKFEGRVRG 67

  Fly  1149 DPKPRVKFYKDEKEILETNDRIQIIRDKDYLGFYELVIADVQKTDAGTYSCKATNKHGEANCEAI 1213
            .|:|:|.:::..:.|  ||.. :.:.|....|.:.|||..|::.|.|.|:|:|:|..| |....:
Mouse    68 YPEPQVTWHRKGQAI--TNGG-RFLLDCGVRGTFSLVIHTVREEDKGKYTCEASNGSG-ARQVTV 128

  Fly  1214 ATTVE----DKNPFGALS------GQILPA--GE------------------------------K 1236
            ..|||    .|.....||      |:..|:  ||                              .
Mouse   129 ELTVEGNSMKKRDQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPP 193

  Fly  1237 PVFQWKRNGEEFDPEERFKVLFGEDEDSLALVFQHVKPEDAGIYTCVAQTSTGNISCSAELSVQG 1301
            |...|.:......|..|  |...|......|..:.|..:|.|:|||:....:|..|.|||||:.|
Mouse   194 PQVTWLKGNVPLQPSAR--VSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSAELSIPG 256

  Fly  1302 ---AIQTLNREPEKPTLVIEHREANASIGGSAILELQCKGFPKPAVQWKHDGEVIQVDDRHKFMY 1363
               |.:...|..:.|:..| .:|....:........:.|..|.|    :..|...:..:.|
Mouse   257 LDNAARLAVRGTKAPSPDI-RKEVTNGVSKDPETVAESKNCPSP----QRSGSSARATNSH---- 312

  Fly  1364 EDEESMSLVIKNVDTVDAGVYTIEAINELGQDESSINLVVKAPPKI-------KKITDITCSAGE 1421
                     :|:                  ..|....|...||.|:       |..:.||..|
Mouse   313 ---------LKS------------------PQEPKPKLCEDAPRKVPQSSILQKSTSTITLQA-- 348

  Fly  1422 TIKMEIEVEGFPQPTVQVTNNGKD-----VTAESNVKISSSSIGKSLEKVVVEVKEIKLSQAGNY 1481
             :|::.|..   .|.:...:.|:|     ...::.:....||:|.|:               ||.
Mouse   349 -LKVQPEAR---VPAIGSFSPGEDRKSLAAPQQATLPTRQSSLGGSV---------------GNK 394

  Fly  1482 SIKATNDLSQTSEYWSC--TVKSKPVIVKNFESEYIHGEKENVQMTVRIDAYPEAKLTWYHDETE 1544
            .:  |.::.:.|:..|.  ..:|:|      :|:.: .|.:.|:....:...|:..:.|:.:...
Mouse   395 FV--TGNIPRESQRESTFPRFESQP------QSQEV-TEGQTVKFICEVSGIPKPDVGWFLEGIP 450

  Fly  1545 IKITDSKYTVSSDGNAYTLKITGATRVDAGKYTVKATNEHGSATSSTQLLI------KCAPEFTH 1603
            ::..:....|..||.::.|.:..|...|:|:|:..|:|..|..:.|..||:      :.||.|:.
Mouse   451 VRRREGITEVYEDGVSHHLCLLRARTRDSGRYSCTASNSLGQVSCSWSLLVDRPNLAQTAPSFSS 515

  Fly  1604 KLKNITVAEGDSNVELVVGVDAYPRPHAKWYIDGIEIDEKRNDFRH-VEEGNDFKLIMNQVATNM 1667
            .||:..|.||...| |...|...|.|...|.::|..|     .|.| :.|....:|.:.......
Mouse   516 VLKDSVVIEGQDFV-LRCSVQGTPAPRVTWLLNGQPI-----QFAHSICEAGVAELHIQDALPED 574

  Fly  1668 QGNYTCKIMNDYGKLEDNCVVTVNCK-------------------PKVKRGLKNVEVQEGKSFTL 1713
            :|.|||...|..|::..:..|||..|                   |...:||.:::|.:|...|:
Mouse   575 RGTYTCLAENAMGQVSCSATVTVQEKKGEGEREHRLSPARSKPIAPIFLQGLSDLKVMDGSQVTM 639

  Fly  1714 EVEVYSEPEAKIKWFKDGHEIYEDARIKISRD---TQRIENYYLTLNLARTEDAGTYEMKATNFI 1775
            .|:|...|..::.|..||:||.|      |.|   .|:...:.|.:.....||.|||..:|.|..
Mouse   640 TVQVSGNPPPEVIWLHDGNEIQE------SEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSA 698

  Fly  1776 GETTSTCKVAVLTSEALSLEQTVTKTLIATTEEPEEGAVPEIVHVDVFQQHSYESVPLKYEVIAT 1840
            ||                    |....:.|.:||.:|..|..:           |.|  ..|.||
Mouse   699 GE--------------------VRTRAVLTVQEPHDGTQPWFI-----------SKP--RSVTAT 730

  Fly  1841 ------------GIPKPEAIWYHDGKPITPDK-HTAITVDGDHYKLEVQSLDLVDAGEYKVVVQN 1892
                        |.|.|...|..||:.::.|. |..:..:.|.:.|.::::....||:|:::::|
Mouse   731 LGQSVLISCAIAGDPFPTVHWLRDGRALSKDSGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKN 795

  Fly  1893 KVGEKSHQGELSLSGIAEY-----RKPI----LTQGPGLKDIKVNKGD-----KVCEPVVFTADP 1943
            :|||.|.|..|.|......     |:|.    |..|.|:.  ....||     :.|.|......|
Mouse   796 RVGECSCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVG--AHGDGDRHGTLRPCWPARGQGWP 858

  Fly  1944 APEIVLLKDGQPVVETNNVKLKVDKKDAENGLVQYTCTLNILEAEIKDSGRYELKVKNKYGELVT 2008
            ..|     ||:.|  ...:|.:|:.:            |:..|| |:.....:|..::..|:.|:
Mouse   859 EEE-----DGEDV--RGLLKRRVETR------------LHTEEA-IRQQEVGQLDFRDLLGKKVS 903

  Fly  2009 SGWIDVLAKPEISGLNDTKCLPGDTICFEALVQANPKPKVSWTRGNENLCNHENCEVIADVDADK 2073
            :..:..         :|.|.:|.:.:.|.|.:|...|||.
Mouse   904 TKTVSE---------DDLKDIPAEQMDFRANLQRQVKPKT------------------------- 934

  Fly  2074 YRLVFQSVSPCEDGKYTITATNSEGRAAVDFNLAVLVEKPTFIVQPESQSIH-----DYRPVSTK 2133
                                                       :..|.:.:|     |:|.|..|
Mouse   935 -------------------------------------------ISEEERKVHSPQQVDFRSVLAK 956

  Fly  2134 VLVHGVPLPTIEWFKDDKPINYEAINKPGKDKLYAKEDTKKGTDQIESVLDIKSFRENDVGAYTC 2198
            ......|:|                         .|...|..|....|||..|....::.|..:.
Mouse   957 KGTPKTPVP-------------------------EKAPPKAATPDFRSVLGGKKKSPSENGGNSA 996

  Fly  2199 VATN-EIGVTKAPFKLAMLSLAPSFVKKLDNALDVLQGEPLVLECCVDGSPLPTVQWLKDGDEVK 2262
            ...| :.|.:..|   |..:.|...:|.:.||......:|:       |:..|| :.||.....|
Mouse   997 EVLNVKAGESPTP---AGDAQAIGALKPVGNAKPAETPKPI-------GNAKPT-ETLKPVGNTK 1050

  Fly  2263 PSESIKISTNPDGLVKLEINSCQPNDSGAYKLIISNPHGEKVALCAVAVKPEEMQPKFLKPITSQ 2327
            |:|::|           .|.:.||:.|                               |||:|:
Mouse  1051 PAETLK-----------PIANAQPSGS-------------------------------LKPVTN- 1072

  Fly  2328 TVVVGEPLK----LEAQVTGFPA--PEVKWYKDGMLLRPSPEINFINSPNGQIGLIIDAAQPLDA 2386
             ....||.|    .::..|..||  .|||..|           |.:|...||:|...:..:|...
Mouse  1073 -AQPAEPQKPVGNAKSAETSKPAGKEEVKEVK-----------NDVNCKKGQVGATGNEKRPESQ 1125

  Fly  2387 GVYKCLIANKGGEIEGVSKVEIVPKESKPVFVAELQDASSIEGFPVKMDIKVVGNPKPKLQWFHN 2451
            |                         |.|||..:|||....||..:.:..:|:.:|...:.|..|
Mouse  1126 G-------------------------SAPVFKEKLQDVHVAEGEKLLLQCQVISDPPATVTWSLN 1165

  Fly  2452 GHEIKPDASHIAIVENPDNSSSLIIEKTAPGDSGLYEVIAQNPEGSTASKAKLYVAPKADETATE 2516
            |..:|  .:...::....:..|:.|||..|.|.|||:.:|:|..|......::.|    |:..|.
Mouse  1166 GKTLK--TTKFIVLAQEGSRFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTV----DDAQTS 1224

  Fly  2517 E---APQFVSALRDVNADEGQELVLSAPFISNPMPEVIWSKDGVTLTPNERLLMTCDGKHIGLTI 2578
            |   ||:..|               ..|  .:.:|.|:.::...|:..
Mouse  1225 ENTKAPEMKS---------------RRP--KSSLPPVLGTESDATVKK----------------- 1255

  Fly  2579 KPAEAADSGNYTCLLANPLGEDSSACNANVRKVYKPPVFTQKISDQQQVFGNNAKIPVTVSGVPY 2643
            |||....:                       |...||...|...||:...|...::...|:|...
Mouse  1256 KPAPKTPT-----------------------KAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQP 1297

  Fly  2644 PDLEWYFQDKPIPKSEKYSIKNDGDHHMLIVNNCEKGDQGVYKCIASNREGKDITQGRLDIVNEI 2708
            ...:|....|.|.:||...::|......|.:....:...|.|..:..|:.|....|..|.:|:
Mouse  1298 ITCKWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVD-- 1360

  Fly  2709 KKHSRSEPPVFLKKIGDCDIYEGMVAKFTACATGYPEPEVEWFKNDQKLFPSDRFLIDIEPNGLL 2773
                :.:||.                 .|.||:                        ||
Mouse  1361 ----KPDPPA-----------------GTPCAS------------------------DI------ 1374

  Fly  2774 RLTIKNVTEYDVGRYSCRIFNPYGDDICHAELFYDSLDSQQKPLEDQYTDFKKYKKSGAPPPLSE 2838
                         |.|....:.||..       ||...:.|
Mouse  1375 -------------RSSSLTLSWYGSS-------YDGGSAVQ------------------------ 1395

  Fly  2839 GPIISRMTDRGLLLSWNPSVPLTPRYPITYQIEMMDLPEGDWRTLRTGVRSCACDIRNLEPFRDY 2903
                                        :|.:|:.|..:..|:.|.| .||.:.::::|.|.|:|
Mouse  1396 ----------------------------SYNVEIWDTEDKVWKELAT-CRSTSFNVQDLLPDREY 1431

  Fly  2904 RFRVRVENKFGVSDPSPYTQTYRQKLVPDPPKTYTYLPPGTDFRPETSPYFPKDFDIERPPHDGL 2968
            :||||..|.:|.|:||   |......|.:.|:.                  |||
Mouse  1432 KFRVRAVNVYGTSEPS---QESELTAVGEKPEE------------------PKD----------- 1464

  Fly  2969 AQAPQFLLREQDISYGVKDHNTELMWFVYGYPKPKMTYYFDDMLIESGGRFDQSYTRNGQATLFI 3033
                     |.::|                          ||
Mouse  1465 ---------EVEVS--------------------------DD----------------------- 1471

  Fly  3034 NKMLDRDVGWYEAVATNEHGEARQRVRLEIAEHPRFLKRPDETFIMARKNGRIEAKLVGIPLPEV 3098
                                                    ||                  ..|||
Mouse  1472 ----------------------------------------DE------------------KEPEV 1478

  Fly  3099 HWFKDWKPIVDSSRIKISSYDPDIYVLSIHDSIIKDGGLYSISARNIAGSISTSVTVHIEENEDQ 3163
                |::                                              :|||:.|:
Mouse  1479 ----DYR----------------------------------------------TVTVNTEQ---- 1489

  Fly  3164 YIYKTYGRHPYVRSKQLRYQDKYDIGDELGRGTQGITYHAVERSSGDNYAAKIM--YGRPELRPF 3226
                             :..|.|||.:.||.|..|..:..||:.:|..:|.|..  |...| :..
Mouse  1490 -----------------KVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKE-KDN 1536

  Fly  3227 MLNELEMMNTFNHKNLIRPYDAYDTDRSVTLIMELAAGGELVRDNLLRRDY-YTERDIAHYIRQT 3290
            :..|:.:||..:|..|::..||::...::.:::|:.:||||. :.::..|: .|||:...|:||.
Mouse  1537 IRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELF-ERIIDEDFELTERECIKYMRQI 1600

  Fly  3291 LWGLEHMHEMGVGHMGLTIKDLLISVVGGDIIKVSDFGLSRKI-NRHNLSTLDYGMPEFVSPEVV 3354
            ..|:|::|:.|:.|:.|..::::.....|..||:.||||:|:: |..:|..| :|.||||:|||:
Mouse  1601 SEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVL-FGTPEFVAPEVI 1664

  Fly  3355 NKEGVNFSHDMWTVGLITYVLLGGHNPFLGIDDRETLTKIREGRWDFKDEIWTHISDDGRDFISR 3419
            |.|.:.::.|||::|:|.|:|:.|.:||:|.:|.|||..:....|||.||.:..||||.:||||.
Mouse  1665 NYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISN 1729

  Fly  3420 LLLYSPEERMDVKTALKHPWFFMLDRPVYDHDYQIGTDRLRNYYDHFRDWYANASCKNYFRRRRL 3484
            ||....:.|:|....|:||| .|.|....:.. ::..||:                |.|..||:
Mouse  1730 LLKKDMKNRLDCTQCLQHPW-LMKDTKNMEAK-KLSKDRM----------------KKYMARRK- 1775

  Fly  3485 SGCFQHPSKMVYPPGHVYTPENTPEPLPEPRIRAKREEVVSKYLHPDYELGLIQSESHYQYGPDT 3549
               :|.....|...|                 |.....::|         ||...:|
Mouse  1776 ---WQKTGNAVRAIG-----------------RLSSMAMIS---------GLSGRKS-------- 1803

  Fly  3550 YLLQLRDVNFPVRLREYMKVAHRRSPSFALNDSVDWSLPVIRERRRFTDIMDEEIDDERTRSRIS 3614
                                 ...||:..:|                    .|:::.|
Mouse  1804 ---------------------STGSPTSPIN--------------------AEKLESE------- 1820

  Fly  3615 MYAANESYSIRRLRTELGPRLDEYTEADAMIETQREGYP---PFFREKPQTIAITENQPSHIHCF 3676
                                 |:.::  |.:|...|..|   |:|.:..:.:.:.|...:...|.
Mouse  1821 ---------------------DDVSQ--AFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCK 1862

  Fly  3677 AVGDPKPCVQWFKNDMVLTESKRIKISVDEDGRSILRFEPALHFDVGVYKVVARNKVGQTVARCR 3741
            ..|.|.|.|.|||:|..:.||:..:|..||||...|........|...|...|.|.:|:......
Mouse  1863 IEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAE 1927

  Fly  3742 IVVATLPD 3749
            ::|.|:.:
Mouse  1928 LIVETMEE 1935

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Unc-89	NP_001097440.1	RhoGEF	90..260	CDD:238091
		PH_unc89	275..388	CDD:270134
		Atrophin-1	<493..690	CDD:331285
		TonB_N	502..>596	CDD:318287
		I-set	1017..1108	CDD:333254
		I-set	1123..1212	CDD:254352	26/88 (30%)
		I-set	<1236..1299	CDD:333254	19/62 (31%)
		I-set	1313..1403	CDD:254352	11/89 (12%)
		Ig_3	1406..1487	CDD:316449	17/92 (18%)
		I-set	1499..1595	CDD:254352	20/95 (21%)
		I-set	1599..1690	CDD:333254	26/91 (29%)
		I-set	1694..1786	CDD:254352	28/94 (30%)
		I-set	<1836..1903	CDD:333254	22/79 (28%)
		I-set	1922..2005	CDD:333254	16/87 (18%)
		I-set	2018..2108	CDD:333254	9/89 (10%)
		I-set	2113..2214	CDD:333254	19/106 (18%)
		I-set	2220..2302	CDD:254352	17/81 (21%)
		I-set	2318..2408	CDD:254352	21/95 (22%)
		I-set	2415..2506	CDD:254352	26/90 (29%)
		I-set	2519..2608	CDD:254352	9/88 (10%)
		I-set	2615..2696	CDD:254352	18/80 (23%)
		I-set	2717..2805	CDD:254352	10/87 (11%)
		FN3	2834..2925	CDD:238020	22/90 (24%)
		I-set	<2996..3063	CDD:333254	2/66 (3%)
		I-set	3067..3157	CDD:333254	9/89 (10%)
		PK_Unc-89_rpt1	3182..3440	CDD:271011	96/261 (37%)
		I-set	3654..3744	CDD:333254	25/89 (28%)
		FN3	3748..3840	CDD:238020	0/2 (0%)
		STKc_Unc-89_rpt2	3893..4151	CDD:271014
Mylk	NP_647461.3	I-set	42..132	CDD:254352	26/93 (28%)
		Ig	42..131	CDD:299845	26/92 (28%)
		I-set	165..254	CDD:254352	19/90 (21%)
		Ig	183..251	CDD:143165	16/69 (23%)
		23ISL	255..409	CDD:293226	34/212 (16%)
		I-set	411..501	CDD:254352	20/96 (21%)
		Ig	428..498	CDD:143165	15/69 (22%)
		I-set	511..597	CDD:254352	26/91 (29%)
		IGc2	524..587	CDD:197706	19/68 (28%)
		I-set	620..709	CDD:254352	29/114 (25%)
		Ig	637..706	CDD:143165	24/94 (26%)
		I-set	718..806	CDD:254352	25/100 (25%)
		Ig	721..806	CDD:299845	24/97 (25%)
		I-set	1129..1218	CDD:254352	26/90 (29%)
		IGc2	1142..1208	CDD:197706	19/67 (28%)
		Ig	1269..1366	CDD:299845	22/102 (22%)
		I-set	1269..1358	CDD:254352	20/88 (23%)
		FN3	1362..1453	CDD:238020	36/213 (17%)
		STKc_MLCK1	1492..1750	CDD:271093	97/261 (37%)
		Pkinase	1495..1750	CDD:278497	96/258 (37%)
		I-set	1840..1930	CDD:254352	25/89 (28%)
		IGc2	1853..1920	CDD:197706	22/66 (33%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	76	1.000	Domainoid score	I8963
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.910