back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Obsc and SPEG

DIOPT Version :10

Sequence 1:	NP_001097440.1	Gene:	Obsc / 3346201	FlyBaseID:	FBgn0053519	Length:	4218	Species:	Drosophila melanogaster
Sequence 2:	XP_011508781.1	Gene:	SPEG / 10290	HGNCID:	16901	Length:	3277	Species:	Homo sapiens

Alignment Length:	3549	Identity:	718/3549 - (20%)
Similarity:	1156/3549 - (32%)	Gaps:	1187/3549 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly  1564 KITGATRVDAGKYTVKATNEHG--------SATSSTQLLIKCAPEFTHKLKNITVAEGDSNVELV 1620
            |..|....|||   .:|....|        .|.....:.:..||.|...|||..|..| |:|.|.
Human     3 KARGTRGEDAG---TRAPPSPGVPPKRAKVGAGGGAPVAVAGAPVFLRPLKNAAVCAG-SDVRLR 63

  Fly  1621 VGVDAYPRPHAKWYIDGIEIDEKRNDFRHVEEGNDFKLIMNQVATNMQGNYTCKIMNDYGKLEDN 1685
            |.|...|:|..:|:.||        ............|.:.:......|.|:|...|:.|:....
Human    64 VVVSGTPQPSLRWFRDG--------QLLPAPAPEPSCLWLRRCGAQDAGVYSCMAQNERGRASCE 120

  Fly  1686 CVVTV-------NCKPKV------------KRGLKNVEVQEGKSF----------------TLEV 1715
            .|:||       :|:.:.            .:|.:.:|:::..:|                |...
Human   121 AVLTVLEVGVFRSCRKQSYDSETAEDDISDVQGTQRLELRDDGAFSTPTGGSDTLVGTSLDTPPT 185

  Fly  1716 EVYSEPEAKIKWFKDGHEIYEDARIKISRDTQRIENYYLTLNLARTEDAGTYEM--------KAT 1772
            .|....|.::.|:..|..:.|. .......|:|:..   :...|:...||...:        ...
Human   186 SVTGTSEEQVSWWGSGQTVLEQ-EAGSGGGTRRLPG---SPRQAQATGAGPRHLGVEPLVRASRA 246

  Fly  1773 NFIGE---TTSTCKVA---------VLTSEALSLEQTVTKTLIATTEEPEEGAVPEIVHVDVFQQ 1825
            |.:|.   :..:..||         :....|||:..:|.::.: ..|||:  ..|::  .....:
Human   247 NLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSVPQSGL-RREEPD--LQPQL--ASEAPR 306

  Fly  1826 HSYESVPLKYEVIATGIPKPEAIWYHDGK--PITPDKHTAITVDGDHYKLEVQSLDLVDAGEYKV 1888
            ...:..|.|..::..  |.|..     ||  |..|....|.|....|.:.:...|      ....
Human   307 RPAQPPPSKSALLPP--PSPRV-----GKRSPPGPPAQPAATPTSPHRRTQEPVL------PEDT 358

  Fly  1889 VVQNKVGEKSHQGELSLSGIAEYR--KPI---------LTQGPGLKDIKVNKGDKVCEPVVF--- 1939
            ..:.|.|:||.....||:|.||.|  .|:         |.:.|.|    |..|.::.:.:.|
Human   359 TTEEKRGKKSKSSGPSLAGTAESRPQTPLSEASGRLSALGRSPRL----VRAGSRILDKLQFFEE 419

  Fly  1940 --------TADPAPEIVLLKDGQPVVETNNVKLKVDKKDAENG---------------------L 1975
                    .:.|||    |:...|:.:..:::....::.|..|                     .
Human   420 RRRSLERSDSPPAP----LRPWVPLRKARSLEQPKSERGAPWGTPGASQEELRAPGSVAERRRLF 480

  Fly  1976 VQYTCTLNILEAEIKDSGRYELKVKNKYGELVTSGWIDVLAKPEISGLNDTKCLPGDTICFEALV 2040
            .|...:|:....:...:...||:...:.|.:..|...:.|.:...|              ..|.:
Human   481 QQKAASLDERTRQRSPASDLELRFAQELGRIRRSTSREELVRSHES--------------LRATL 531

  Fly  2041 QANPKPKVSWTRGNENLCNHENCEVIADVDADKYRLVFQSVSPC---------------EDGK-- 2088
            |..|.|:   ..|...|.:..:....:           ::|||.               |.|:
Human   532 QRAPSPR---EPGEPPLFSRPSTPKTS-----------RAVSPAAAQPPSPSSAEKPGDEPGRPR 582

  Fly  2089 -----------------------YTITATNS--EGRA-----AVDFNLAVLVEKPTFIVQPESQS 2123
                                   :.:|.:.:  |.|:     |.|...|.....|....:|.:|:
Human   583 SRGPAGRTEPGEGPQQEVRRRDQFPLTRSRAIQECRSPVPPPAADPPEARTKAPPGRKREPPAQA 647

  Fly  2124 IHDYRPVSTKVLVHGVPLP-TIEWFKDDKPINYEAINKPGKDKLYAKEDTK------KGTDQIES 2181
            :. :.|.:|..| .|..:| |:|..:............|.:|..:...|.:      ||.....:
Human   648 VR-FLPWATPGL-EGAAVPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGARSQGKGRRARPT 710

  Fly  2182 VLDIKSFRENDVGAYTCVATNEIGVTKAPFKLAMLSLAPSFVKKLDNALDVLQGEPLVLECCVDG 2246
            ..:::|..::.|.|           .:.|.:      ||.|...|.|.: |..|..::|:|.:..
Human   711 SPELESSDDSYVSA-----------GEEPLE------APVFEIPLQNVV-VAPGADVLLKCIITA 757

  Fly  2247 SPLPTVQWLKDGDEVKPSESIKISTNPDGLVKLEINSCQPNDSGAYKLIISNPHGEKVALCAVAV 2311
            :|.|.|.|.|||..::....:.:....:....| :...:..|:|:|....:|..|:.....::.|
Human   758 NPPPQVSWHKDGSALRSEGRLLLRAEGERHTLL-LREARAADAGSYMATATNELGQATCAASLTV 821

  Fly  2312 KPEEMQPKFLKPITSQTVVVGEPLKLEAQVTGFPAPEVKWYKDGMLLRPSPEINFINSPNGQIGL 2376
            :|......|..||||....:..|.:       ||.|...|.:. ..::|||..|..:|..|
Human   822 RPGGSTSPFSSPITSDEEYLSPPEE-------FPEPGETWPRT-PTMKPSPSQNRRSSDTG---- 874

  Fly  2377 IIDAAQPLDAGVYKCLIANKGGEIEGVSKVEIVPKESKPVFVAELQDASSIEGFPVKMDIKVVGN 2441
                                              .::.|.|...|.|.|..||..|.|.|:|.|.
Human   875 ----------------------------------SKAPPTFKVSLMDQSVREGQDVIMSIRVQGE 905

  Fly  2442 PKPKLQWFHNGHEIKPDASHIAIVENPDNSSSLIIEKTAPGDSGLYEVIAQNPEGSTASKAKLYV 2506
            |||.:.|..|...::||....| .|.......|.|.....||:|.|...|.|..|:...:|:|.|
Human   906 PKPVVSWLRNRQPVRPDQRRFA-EEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEV 969

  Fly  2507 A--PKADETATEEAPQFVSALRDVNADEGQELVLSAPFISNPMP-EVIWSKDGVTLTPNERLLMT 2568
            .  |::...|.      ::.|:||:...| |:.|....::.|.. ||.|...|..|.|   .|:.
Human   970 RAHPESRSLAV------LAPLQDVDVGAG-EMALFECLVAGPTDVEVDWLCRGRLLQP---ALLK 1024

  Fly  2569 C----DGKHIGLTIKPAEAADSGNYTCLLANPLGEDSSACNANVRKVYKP---PVFTQKISDQQQ 2626
            |    ||:...|.:......|||.|||.|:.  .:|...|:|  |...:|   |:||:.:.|.:.
Human  1025 CKMHFDGRKCKLLLTSVHEDDSGVYTCKLST--AKDELTCSA--RLTVRPSLAPLFTRLLEDVEV 1085

  Fly  2627 VFGNNAKIPVTVSGVPYPDLEWYFQDKPIPKSEKYSIKNDGDHHMLIVNNCEKGDQGVYKCIASN 2691
            :.|..|:....:||.|.|.:.|.....|:.:||...::.||..|.|.:.:....|:|:|...|.|
Human  1086 LEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVN 1150

  Fly  2692 REGKDITQGRLDIVNEIKKHSRSEP------------------------PVFLKKIGDCDIYEGM 2732
            ..|:.....:|.:  |..:.:.|.|                        |.||:.:.|.::....
Human  1151 THGQAHCSAQLYV--EEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAK 1213

  Fly  2733 VAKFTACATGYPEPEVEWFKNDQKLFPSDRFLI----DIEPNGLLRLTIKNVTEYDVGRYSCRIF 2793
            .|......||.|.|.:.||.|..::..||...:    |:.     ||....|.....|.|...|.
Human  1214 EAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVH-----RLVFPAVGPQHAGVYKSVIA 1273

  Fly  2794 NPYGDDICHAELFYDSLDSQQKPLEDQYTDFKKYKKSGAPPPLSEGPIISRMTDRGLLLSWNPSV 2858
            |..|...|:|.|:              .||.       .|.|....|.:..:|.|.:.|:|||
Human  1274 NKLGKAACYAHLY--------------VTDV-------VPGPPDGAPQVVAVTGRMVTLTWNP-- 1315

  Fly  2859 PLTPRY--------PITYQIEMMDLPEGDWRTLRTGVRSCACDIRNLEPFRDYRFRVRVENKFGV 2915
               ||.        .:||.::...|....|..|.||:|........|.....:.|||
Human  1316 ---PRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRV-------- 1369

  Fly  2916 SDPSPYTQTYRQKLVPDPPKTYTYLPPGTDFRPETSPYFPKDFDIERPPHDGLAQAPQFLLREQD 2980
                 .:.|.:....|.||.....|                   :|..|  .|.:||. :|.:.|
Human  1370 -----LSTTVKSSSKPSPPSEPVQL-------------------LEHGP--TLEEAPA-MLDKPD 1407

  Fly  2981 ISYGVKDHNTELMWFVYGYPKPKMTYYFDDM------------LIES-GGRFDQSYTRNGQATLF 3032
            |.|.|:           |.| ..:|..|:.:            |:|: .|.::.|...:.|..|.
Human  1408 IVYVVE-----------GQP-ASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLR 1460

  Fly  3033 INKMLDRDVGWYEAVATNEHGEARQRVRLEIAEHPRFLKRPDETFIMARKNGRIEAKLVGIPLPE 3097
            |.::..||:|.....|.|.||.....|.||:||.|||....::..:.|.:..|....:.|.|||:
Human  1461 ICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPD 1525

  Fly  3098 VHWFKDWKPIVDSSRIKISSYDPDIYVLSIHDSIIKDGGLYSISARNIAGSIS--TSVTVH---- 3156
            :.|:||...:.:||.:.. .|:.:...|.:..:..:|||:|:.:|:|:||.:|  ..:.||
Human  1526 IMWYKDEVLLTESSHVSF-VYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQT 1589

  Fly  3157 ------IEENEDQYIYKTYGRHPYVRSKQLRYQDKYDIGDELGRGTQGITYHAVERSSGDNYAAK 3215
                  :.|:||.     .||         |..|.|||..|:|||........||||||..:|||
Human  1590 AMEVEGVGEDEDH-----RGR---------RLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAK 1640

  Fly  3216 IMYGRPELRPFMLNELEMMNTFNHKNLIRPYDAYDTDRSVTLIMELAAGGELVRDNLLRRDYYTE 3280
            .:..:.:.:.....|..::....|..::..::|::..|.:.::.||..  |.:.:.:.|:....|
Human  1641 FIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCT--EELLERIARKPTVCE 1703

  Fly  3281 RDIAHYIRQTLWGLEHMHEMGVGHMGLTIKDLLI--SVVGGDIIKVSDFGLSRKINRHNLSTLDY 3343
            .:|..|:||.|.|:.::|:..|.|:.:..::||:  ...|...:::.|||.::::.........|
Human  1704 SEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQY 1768

  Fly  3344 GMPEFVSPEVVNKEGVNFSHDMWTVGLITYVLLGGHNPFLGIDDRETLTKIREGRWDFKDEIWTH 3408
            |.||||:||:||:..|:...|:|.||::.::.|.|.:||:|.:||.||..||.....|::..:..
Human  1769 GTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLS 1833

  Fly  3409 ISDDGRDFISRLLLYSPEERM--DVKTALKHPWFFMLDRPVYDHDYQIGTDRLRNYYDHFRDWYA 3471
            :|.:.|.|:.::|:   ::|:  ..:..|:||||     .......::.||.|:.:....|...:
Human  1834 LSREARGFLIKVLV---QDRLRPTAEETLEHPWF-----KTQAKGAEVSTDHLKLFLSRRRWQRS 1890

  Fly  3472 NASCKNYFRRRRLSGCFQHPSKMVY-------PPG------------------------------ 3499
            ..|.|.:...|.:....:.|.:.|:       ||.
Human  1891 QISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSG 1955

  Fly  3500 -------------HVYTPEN----------------------TPEPLPEP--------------- 3514
                         .:.|||.                      :||.||.|
Human  1956 SRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGEL 2020

  Fly  3515 -------------------------------------------------------RIRAKREEVV 3524
                                                                   |::|.|:.::
Human  2021 RRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLL 2085

  Fly  3525 -------------------------------------SKYLHPDYELGLIQSESHYQYG------ 3546
                                                 :.:..|..|...:|..|.:..|
Human  2086 RGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRG 2150

  Fly  3547 ------------------------PDTYLLQLRDVNFPVR------------------------- 3562
                                    |....|.....:.|.|
Human  2151 RHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQP 2215

  Fly  3563 ------------------------------------LREYMKV-------AHRRSPS-------- 3576
                                                |..|.::       .|.:.||
Human  2216 PAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPS 2280

  Fly  3577 --------FA---------------------------------------LNDSVD---------- 3584
                    ||                                       |:.|::
Human  2281 EPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEA 2345

  Fly  3585 ----------------------------------------------------------------- 3584

Human  2346 KFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAV 2410

  Fly  3585 ----------------------------WSL-----------------PVIRERRRFTDIMDEEI 3604
                                        |..                 ||:..|||.:..:
Human  2411 GEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTL---- 2471

  Fly  3605 DDERTRSRIS----------------------MYAANESYSIRRL-------------------- 3627
              ||..||:.                      ..|.:|..|:|||
Human  2472 --ERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAES 2534

  Fly  3628 -----------------RTEL-----GPRLDE-YTEADAMIETQRE----------GYPPFFREK 3659
                             |:.|     .||.|: .:..:.....|.|          .:||.|..|
Human  2535 LGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIK 2599

  Fly  3660 PQTIAITENQPSHIHCFAVGDPKPCVQWFKNDMVLTESKRIKISVDEDGRSILRFEPALHFDVGV 3724
            .:...:.|.:.:.:.|.....|.|.:.|.|:...|.....:.|...:|||.:|....|.....|:
Human  2600 LKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGL 2664

  Fly  3725 YKVVARNKVGQTVARCRIVVATLPDAPDSPEISANSGTEILLRWKQPRDDGHSTVLC-YSLQYKL 3788
            |:..|.|.:|...:.|.:.||.:|.....||:........|:.||    .|.|...| |:|:.::
Human  2665 YECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWK----PGDSRAPCTYTLERRV 2725

  Fly  3789 SNCDAWTTVADNIDHEFYLLHDLQPNTNYQFRLASKNRIG----------------WSEMGIPVS 3837
            .....|..|:..|...:|.:..|......:||:|..||.|                .....:|.:
Human  2726 DGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSA 2790

  Fly  3838 AS-----------------------------------TVGGDAPKIHITKAMKHLQQL------- 3860
            |.                                   ||...:|....::|:..|:.:
Human  2791 AHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTP 2855

  Fly  3861 --TENGHQVV-PEEERVHTDYHCEREP-------------------------------------- 3884
              ...|.|.. |.|..:.:.:....||
Human  2856 PRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAP 2920

  Fly  3885 -------------PNWVTDSSVS------------------------------DKYSFISEIARG 3906
                         |..||..|:|                              ..|:|:.|.|||
Human  2921 PAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARG 2985

  Fly  3907 EFSTIVKGIQKSTDTVVVAKILEVTDENEDNVVAEFDNFKTLRHERIPALFSAYKPLNVPIAIFV 3971
            .|..:....:.:|....||||:....|.:..|:.|::..:||.||||.:|..||  :.....:.:
Human  2986 RFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAY--ITPRYLVLI 3048

  Fly  3972 MEKLQGADVLTYFSSRHEYSEQMVATVVTQLLDALQYLHWRGYCHLNIQPDNVVMASVRSIQVKL 4036
            .|.....::|...|.|..|||..|||.:.|||..|.|||.....||:|:|||:::|...::  |:
Human  3049 AESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNAL--KI 3111

  Fly  4037 VDFGSAKKVN-----KLGMKVTPCGSLDFQPPEMINDEPIFPQSDIWSLGALTYLLLSGCSPFRG 4096
            ||||||:..|     .||.:.   |:|:|..|||:..|||...:|||..|.|||::|||.|||..
Human  3112 VDFGSAQPYNPQALRPLGHRT---GTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYE 3173

  Fly  4097 ADEYETKQNISFVRYRFENLFKEVTPEATRFIMLLFKRHPTKRPYTEDCLEHRWLMSSDYMVRKR 4161
            .|..||:..|...|:....|:...:..||.|:..:...||..||..:|||.|.||..:..|..:|
Human  3174 PDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRR 3238

  Fly  4162 ERAIFLGSRLKTFCDEYHDLKNASATSSKV-LNTVAGGP 4199
            :...|..:|||.|..|....:..:||..|| |.:..|||
Human  3239 QTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP 3277

Known Domains:

Indicated by green bases in alignment.

Software error:

Illegal division by zero at /www/www.flyrnai.org/docroot/cgi-bin/DRSC_prot_align.pl line 592.

For help, please send mail to the webmaster (ritg@hms.harvard.edu), giving this error message and the time and date of the error.