DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Hrs and HGS

DIOPT Version :9

Sequence 1:	NP_525099.3	Gene:	Hrs / 33458	FlyBaseID:	FBgn0031450	Length:	760	Species:	Drosophila melanogaster
Sequence 2:	NP_004703.1	Gene:	HGS / 9146	HGNCID:	4897	Length:	777	Species:	Homo sapiens

Alignment Length:	837	Identity:	343/837 - (40%)
Similarity:	448/837 - (53%)	Gaps:	147/837 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 SFDKNLENATSHLRLEPDWPSILLICDEINQKDVTPKNAFAAIKKKMNSPNPHSSCYSLLVLESI 69
            :|::.|:.|||.|.||.||.|||.|||.|.|.|...|.|..:||||:|..|||.:.|:|.|:||:
Human     7 TFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESV 71

  Fly    70 VKNCGAPVHEEVFTKENCEMFSSFLESTPHENVRQKMLELVQTWAYAFRSSDKYQAIKDTMTILK 134
            |||||..||:||..|:..|.....|:.....|||.|:|.|:|.||:|||:..||:.::||..|:|
Human    72 VKNCGQTVHDEVANKQTMEELKDLLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMK 136

  Fly   135 AKGHTFPELREADAMFTADTAPNWADGRVCHRCRVEFTFTNRKHHCRNCGQVFCGQCTAKQCPLP 199
            .:||.|||.:|:||||.|:.||:|.|...||||||:|....||||||.|||:|||:|::|...:|
Human   137 VEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVMTRKHHCRACGQIFCGKCSSKYSTIP 201

  Fly   200 KYGIEKEVRVCDGCFAALQRPTSGSGGAKSGPRPADSELPAEYLNSTLAQQVQTPARKTEQELKE 264
            |:|||||||||:.|:..|.|...|..       .:.:|||.|||.|.|:||.|.|.::.|..|:|
Human   202 KFGIEKEVRVCEPCYEQLNRKAEGKA-------TSTTELPPEYLTSPLSQQSQLPPKRDETALQE 259

  Fly   265 EEELQLALALSQSEAEQQKPKLQSLPPAAYRMQQRSPSPEAPPEPKEYHQQP-------EEATNP 322
            ||||||||||||||||:::...|.....:|...:..||..:.|.....:..|       .|..:|
Human   260 EEELQLALALSQSEAEEKERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAEDIDP 324

  Fly   323 ELAKYLNRSYWEQRKISESSSMASPSAPSPM-PPTPQP--------------------QQIMPL- 365
            |||:||||:||| :|..|:....:||||.|: .|..||                    |.|.|.
Human   325 ELARYLNRNYWE-KKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVENPLPETDSQPIPPSG 388

  Fly   366 ------QVKSAD-EVQIDEFAANMRTQVEIFVNRMKSNSSRGRSISNDSSVQTLFMTLTSLHSQQ 423
                  |..:.: |...::|...::..|..||||||||..|||||:|||:|.:||.::..:|.|.
Human   389 GPFSEPQFHNGESEESHEQFLKALQNAVTTFVNRMKSNHMRGRSITNDSAVLSLFQSINGMHPQL 453

  Fly   424 LSYIKEMDDKRMWYEQLQDKLTQIKDSRAALDQLRQEHVEKLRRIAEEQERQRQMQMAQKLDIMR 488
            |..:.::|::|::||.|||||.||:|:|.||..||:||.|||||.|||.|||||:|:||||:|||
Human   454 LELLNQLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMR 518

  Fly   489 KKKQEYLQYQRQLALQRIQEQEREMQLRQEQQKAQYLMGQSAP--PFPYMPPSAVPQHGSPSHQL 551
            :||||||:.|||||:||:||||:|.|:|.||||....|....|  |.||....|:|
Human   519 QKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMP--------- 574

  Fly   552 NNVYNPYAAGVPGYLPQGPAPAPNGHGQFQAIPPGMYNPAIQQPMPPNLQPGGLMQQPAPPGNPQ 616
                   |||...|.|.|||..|:           .::||......|  ..|..|.||||...|
Human   575 -------AAGGVLYQPSGPASFPS-----------TFSPAGSVEGSP--MHGVYMSQPAPAAGP- 618

  Fly   617 MMPPMPENQFANNPAAI------------LQLPQQHS--IAQPPQIPFQPQP----QQIPGQQPQ 663
             .|.||..  |.:|:.:            ...||..:  .|.|....:||.|    |.:..|.||
Human   619 -YPSMPST--AADPSMVSAYMYPAGATGAQAAPQAQAGPTASPAYSSYQPTPTAGYQNVASQAPQ 680

  Fly   664 QIP--GQQPQQIP-----------GQQP---QQIPGQQPQQ---IPVQQPQ---PQPQMGHVMLQ 706
            .:|  .|.||...           |.||   |.:....|.|   :|.|||.   .||      :.
Human   681 SLPAISQPPQSSTMGYMGSQSVSMGYQPYNMQNLMTTLPSQDASLPPQQPYIAGQQP------MY 739

  Fly   707 QHQAP---PAAQAPPVTEIANNQVQAVAAAPAPPQNEPGPAPVKAEEPATAELISFD 760
            |..||   |..|.|||.:              .||.:..||     :.:.|:|||||
Human   740 QQMAPSGGPPQQQPPVAQ--------------QPQAQGPPA-----QGSEAQLISFD 777

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hrs	NP_525099.3	VHS_Hrs_Vps27p	2..143	CDD:239626	68/137 (50%)
		FYVE_Hrs	157..217	CDD:277260	35/59 (59%)
		Hrs_helical	377..471	CDD:289018	49/93 (53%)
		GBP_C	<456..520	CDD:303769	46/63 (73%)
		coiled coil	501..512	CDD:293879	8/10 (80%)
HGS	NP_004703.1	VHS_Hrs_Vps27p	4..145	CDD:239626	68/137 (50%)
		FYVE_Hrs	159..219	CDD:277260	35/59 (59%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	223..319		38/102 (37%)
		Interaction with SNX1. /evidence=ECO:0000250	225..543		146/325 (45%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	338..407		15/68 (22%)
		Hrs_helical	407..501	CDD:289018	49/93 (53%)
		Interaction with SNAP25 and TRAK2. /evidence=ECO:0000250	445..543		60/97 (62%)
		Interaction with STAM. /evidence=ECO:0000250\|UniProtKB:Q99LI8	454..572		70/117 (60%)
		Interaction with NF2. /evidence=ECO:0000269\|PubMed:10861283	480..777		126/354 (36%)
		GBP_C	<486..560	CDD:303769	50/73 (68%)
		DUF1421	519..>746	CDD:284607	85/265 (32%)
		coiled coil	531..542	CDD:293879	8/10 (80%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	718..777		24/83 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165147338
Domainoid	1	1.000	133	1.000	Domainoid score	I5104
eggNOG	1	0.900	-	-		E1_KOG1818
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H37954
Inparanoid	1	1.050	541	1.000	Inparanoid score	I1187
Isobase	1	0.950	-	0.811278	Normalized mean entropy	S3060
OMA	1	1.010	-	-		QHG45851
OrthoDB	1	1.010	-	-		D334336at33208
OrthoFinder	1	1.000	-	-		FOG0005737
OrthoInspector	1	1.000	-	-		oto90089
orthoMCL	1	0.900	-	-		OOG6_106317
Panther	1	1.100	-	-	LDO	PTHR46275
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X4133
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.720