Protein Alignment mio and SEA4

DIOPT Version :10

• Sequence 1: NP_608656.1
• Gene: mio / 33399
• FlyBaseID: FBgn0031399
• Length: 867
• Species: Drosophila melanogaster
• Sequence 2: NP_009446.2
• Gene: SEA4 / 852170
• SGDID: S000000200
• Length: 1038
• Species: Saccharomyces cerevisiae
• Alignment Length: 973
• Identity: 230/973 - (23%)
• Similarity: 396/973 - (40%)
• Gaps: 251/973 - (25%)

  Fly    78 ADGKVG-ICNFRDTY-----DSSWEYTPRQQRMCTCLAWNELDAN---ILAIGHDRHRNDTCITI 133
            |:..|| :.|.:||.     :::::...|.::. .|:  |.|..|   ::|:|.||:::|:.:.|
Yeast   117 AENIVGSVSNLKDTQGYPVSETNYDIRVRAKKQ-RCI--NSLGINTNGLIAMGLDRNKHDSSLQI 178

  Fly   134 WDIE-RGVPKETAN---FFGVGESANSICWDRNHRTVIAGMSQKMIKLFDLRQSNATCQSIQTKT 194
            ||:. .....||.|   .:...||..|:.: .|..:|:|. |.|.:|..|:|..|...|. .|:.
Yeast   179 WDMNYHDDSHETINPMFSYCTNESIVSLKF-LNDTSVLAA-STKFLKEIDVRSPNPIYQH-PTRL 240

  Fly   195 VQGLSVSPNGNYLCSYV--DSVITLWDPR-----------NIKSPLRQIQ-------SSKNHLQ- 238
            ...:.::|..::..|..  |..:.:||.|           |:.|||...:       :|:.::.
Yeast   241 TYDIKLNPFNDWQFSTYGDDGTLAIWDRRKLSDQASLGDLNVASPLLTFEKLVGSGAASRKYMNS 305

  Fly   239 -IAWCPTRTSLLSSLQR-------------DSSYITLYDIRSVDTDNSGEIYHV-KRQISPFPAR 288
             ..|...|.:..::|.|             ||:    .|| :.|.||...|.:: ...:......
Yeast   306 CFRWSCVRNNEFATLHRGDTIKRWRLGYYCDSN----RDI-AADDDNEMNIENLFVSSVHDTNTM 365

  Fly   289 YQHSGKFSFVN---------CLSWHSRDF---------------ERALLLADALN--ILDFRLPA 327
            |.....|.::.         |:......:               ..:|||::..|  |.:.|
Yeast   366 YDRVATFDYIPRSNNGTSLICMRQSGTIYRMPISEVCSKAILNNRNSLLLSNFENTEIDEIR--- 427

  Fly   328 TLHTAH--SNRRKLPLLMQRPLYTPASPTSTAATPTQQQPTS-------SCSTNSGSS--LDFST 381
             ::..|  ||...:..:::...:.....:.........:|.:       |...|.||:  || |.
Yeast   428 -VNNEHEKSNLENVKTILKNLSFEDLDVSEDYFPSGHDEPNNEIEYSELSEEENEGSNDVLD-SK 490

  Fly   382 PGGSPFNVDLLKPE-LFELDLVDETRQRALEDYGIKP-------DNKRFGELHLTPYLRNVWSTL 438
            .|...|    .||| |.|.|:....|.||...||:.|       |:.:  .|....|:||.|..:
Yeast   491 RGFELF----WKPEKLLEKDISVIMRTRASLGYGLDPMNTVEMIDSSK--NLQNNAYIRNTWRWI 549

  Fly   439 ---------NNVYSEDRLTGLKATLGINLG---------HTSEALMASSRIESQVLQWPEGINNS 485
                     ..:.|.|...|.:..:||..|         :..|.:::..::..::
Yeast   550 AIAKASVDDGTMVSGDLDLGYEGVIGIWNGINGISNQDRYRQETILSDKQLNKEM---------- 604

  Fly   486 NKLICYRSEQRD------------------LALQLCGWAFEQELDR--FIDQ---LYANKEYSRA 527
            .|:|..|.:.||                  |.|.:.||    :|.|  :.|:   :..|..|.:|
Yeast   605 EKIIKLRRKNRDRNSPIANAAGSPKYVQRRLCLIISGW----DLSRSDYEDKYNIIMKNGHYEKA 665

  Fly   528 AMICVFHLKIFHACNILSSAADNMRDPSMYRITVIALSSFNADR---CSSTWRNQRSSANMQIHD 589
            |...||...|..|..||.||     .....|:...|::.:.|.:   .::.||.|....:.::.|
Yeast   666 AAWAVFFGDIPKAVEILGSA-----KKERLRLIATAIAGYLAYKDLPGNNAWRQQCRKMSSELDD 725

  Fly   590 PHLRAVFSFLTMEKDNFDAVLKEEGVSLSDRMAFACKYLSETKLADYVAQQIQAAIDGGDLNGLL 654
            |:||.:|:|:. :.|.:| :|.|..:||.:|:..|.::|::|.|..::.:.....|:.|:|.||:
Yeast   726 PYLRVIFAFIA-DNDWWD-ILYEPAISLRERLGVALRFLNDTDLTTFLDRTSSTVIENGELEGLI 788

  Fly   655 LTGESQDGIDILQSYMDTSFDVQTVALVAI----NYFRQELFEDKRIQYWIASYLDHLNSWGLWE 715
            |||.:.:|||:||||::.:.|||:.||::|    .|||     |:|:..||.:|.|.|.||.|:.
Yeast   789 LTGITPNGIDLLQSYVNKTSDVQSAALISIFGSPRYFR-----DQRVDEWIQTYRDMLKSWELFS 848

  Fly   716 KRAELDI---KIE---------SIRPSSRSSRTVFLSCNFCGKSVSNALLDEPRPRSTTTSTNRL 768
            .||..|:   |:.         .|:|     |.:::.|..|.::::......|....:|::.|..
Yeast   849 MRARFDVLRSKLSRTKTGVLTADIKP-----RQIYIQCQNCKQNINTPRTSSPSSAVSTSAGNYK 908

  Fly   769 SS--------------------------------CPSCRKPLPRCSLCLMHMGT-----MVNMSN 796
            :.                                ||.|....|||::|||.:||     ::|.:.
Yeast   909 NGEAYRRNNADYKKFNTGSSEAQAADEKPRHKYCCPHCGSSFPRCAICLMPLGTSNLPFVINGTQ 973

  Fly   797 GETPTTTPDVPGWQTK-------PFSKWFSWCQTCRHGGHTEHIMQWFKQNSECPVSSCNCRC 852
            ...|..|.|......:       ..::|||:|.:|.||.|..|..:||.:::.||...|.|:|
Yeast   974 SRDPMQTEDSQDGANRELVSRKLKLNEWFSFCLSCNHGMHAGHAEEWFDRHNVCPTPGCTCQC 1036

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mio	NP_608656.1	WD40 repeat	8..44	CDD:293791
		WD40	60..>266	CDD:475233	55/235 (23%)
		WD40 repeat	60..101	CDD:293791	6/28 (21%)
		WD40 repeat	105..148	CDD:293791	15/49 (31%)
		WD40 repeat	155..188	CDD:293791	10/32 (31%)
		WD40 repeat	195..231	CDD:293791	10/48 (21%)
		WD40 repeat	236..284	CDD:293791	12/63 (19%)
		mRING-H2-C3H3C2_Mio	778..852	CDD:438352	27/85 (32%)
SEA4	NP_009446.2	zf-RING_16	<943..1036	CDD:390276	30/92 (33%)