DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and ROBO3

DIOPT Version :9

Sequence 1:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster
Sequence 2:	NP_071765.2	Gene:	ROBO3 / 64221	HGNCID:	13433	Length:	1386	Species:	Homo sapiens

Alignment Length:	1457	Identity:	417/1457 - (28%)
Similarity:	604/1457 - (41%)	Gaps:	303/1457 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    23 PRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPLKIL---PGSHRITLPAGGLFFLKV 84
            |||||.|.|..|.|.|||||.|:|||.|.|.|:|||:|..:..:   |.:||:.||:|.|||.::
Human    64 PRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAHRLLLPSGALFFPRI 128

  Fly    85 VNSRR-ETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECAAPRGI 148
            |:.|| ..|.|:|.|.|:|.||.|.||||:|:||||||:||..|.|..:|.|:.|:|||..|||.
Human   129 VHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVLECVPPRGH 193

  Fly   149 PEPTVTWKKGGQKL-DLEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLATLKVH 212
            |||:|:|:|.|.:| :.||...:|   ||.|.:....::|.|.|.|:|.|..|.|||:.|.:.|.
Human   194 PEPSVSWRKDGARLKEEEGRITIR---GGKLMMSHTLKSDAGMYVCVASNMAGERESAAAEVMVL 255

  Fly   213 VKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLEDRSLRLERVTI 277
            .:|..:|.|.:|.||..|.|||.|.|.|||.|.:.|.:  ..|.:|..|..:..|.||.:..|:.
Human   256 ERPSFLRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRK--EDGELPTGRYEIRSDHSLWIGHVSA 318

  Fly   278 ADEGEYSCEADNVVGAITAMGTLTVYAPPKFIQRPASKSVELGADTSFECRAIGNPKPTIFWTIK 342
            .|||.|:|.|:|.||...|.|:|:|:.||:.:.:|..:....|...:|:|...|||.|.|||..:
Human   319 EDEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKE 383

  Fly   343 NNSTLIFP--GAPPLDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITSRVQLSLDS 405
            .:..|:||  ...|..||    :......|.:|..||.|....: |.|::...||.::..|.:..
Human   384 GSQVLLFPSQSLQPTGRF----SVSPRGQLNITAVQRGDAGYYV-CQAVSVAGSILAKALLEIKG 443

  Fly   406 QE-DRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQPSS-KLNITTSGDLII 468
            .. |..||:|:.||.||||.:.|...|.|:..|.|.|::.|.:||..:|... :.....:|.|.|
Human   444 ASLDGLPPVILQGPANQTLVLGSSVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQFKTMANGTLYI 508

  Fly   469 SDLDRQQDQGLYTCVASSRAGKSTWSGFLRI--------ELPTNPNIKFYRAPEQTKCPSAPGQP 525
            ::: ::.|.|.|:|||.|..|::||||:|::        :.||.|:     :|     |.||.||
Human   509 ANV-QEMDMGFYSCVAKSSTGEATWSGWLKMREDWGVSPDPPTEPS-----SP-----PGAPSQP 562

  Fly   526 KILNATASALTIVWPTSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVESLTQGAAYM 590
            .:...|.:::|:.|..:.:.|| :...|.:|.:......||..:|..:.....||..|.....|:
Human   563 VVTEITKNSITLTWKPNPQTGA-AVTSYVIEAFSPAAGNTWRTVADGVQLETHTVSGLQPNTIYL 626

  Fly   591 FIVRAENSLGFSPPSPISEPITAGKLVGVRDGS-----ESTGTSQLLLSDVETLLQANDVVELLE 650
            |:|||..:.|.|.|||:|||      |..:|.|     |.....|..|::|        .|.|.|
Human   627 FLVRAVGAWGLSEPSPVSEP------VRTQDSSPSRPVEDPWRGQQGLAEV--------AVRLQE 677

  Fly   651 ANASDSTTARLSWDIDSG-QYIEGFYLYAREL--HSSEYKMVTLLNKGQGLSSCTVPGLAKASTY 712
            .......|.::||.:|.. |.::||.:..|..  ....:.|:.|.:..|  .|..:.||...:..
Human   678 PIVLGPRTLQVSWTVDGPVQLVQGFRVSWRVAGPEGGSWTMLDLQSPSQ--QSTVLRGLPPGTQI 740

  Fly   713 EFFLVPFYKSIVGKPSNSRRMRTLEDVPEAPPYGMEAIQF---NRTSVFLKWLPPQPNRTRNGIL 774
            :..:....:..:|..|.|......|:.|..||.|: |:..   ..:|:.:.|.||.|:: :||::
Human   741 QIKVQAQGQEGLGAESLSVTRSIPEEAPSGPPQGV-AVALGGDGNSSITVSWEPPLPSQ-QNGVI 803

  Fly   775 TSYNVVVKGLDVHNTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAVLRI 839
            |.|.:...|    |.:|...|.:....|.:.:|..|..|:.|...|||||..|||..|.|.::::
Human   804 TEYQIWCLG----NESRFHLNRSAAGWARSAMLRGLVPGLLYRTLVAAATSAGVGVPSAPVLVQL 864

  Fly   840 DARTQSLDTGYTRYPISRDIADDFLTQTWFIVLLGSIIAIIVFL------LGALVL-------FK 891
                          |...|:...........|.|..::....||      .|||:|       ::
Human   865 --------------PSPPDLEPGLEVGAGLAVRLARVLREPAFLAGSGAACGALLLGLCAALYWR 915

  Fly   892 RYQFIKQTSLGSLHGNHAIGTVRKFPTLPLNGGGAVGAGGSNSTAAAGQSGHGLWIDPTSGVW-- 954
            |.|   :..|.....:.|......||       .:.|..|::|....|       :.|....|
Human   916 RKQ---RKELSHYTASFAYTPAVSFP-------HSEGLSGASSRPPMG-------LGPAPYSWLA 963

  Fly   955 ------RQAAGGGNPGGVGSCTTKEQLPG-----------YAQATAQQGQQPTLLPDYERLSPLN 1002
                  .::.....|.  |||......|.           |...||:....|...|.|..:.|
Human   964 DSWPHPSRSPSAQEPR--GSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPGEGPVYSTIDP-- 1024

  Fly  1003 MPDYAEVACSTFKSPTHGAPPMGGGGGGQSASLY--------DSCGAYATTNVVANVKLYQNRYA 1059
                |.....||    ||..|....|.....|.|        || ||..     ..|||     .
Human  1025 ----AGEELQTF----HGGFPQHPSGDLGPWSQYAPPEWSQGDS-GAKG-----GKVKL-----L 1070

  Fly  1060 TKPNQSSGSNNNNHQGNDYQSTGMYSAPPSAHYGCLEPKQQQQQ------------------QQP 1106
            .||.|....|         ....:...|||....|||..:::.:                  .:|
Human  1071 GKPVQMPSLN---------WPEALPPPPPSCELSCLEGPEEELEGSSEPEEWCPPMPERSHLTEP 1126

  Fly  1107 N-----LMTTS------------TASTAILTNSPAKVKKINITENKMDQLEGKSER-----TNPF 1149
            :     |:|.|            ..|||.||.||....:   ....|..|.....|     ::|.
Human  1127 SSSGGCLVTPSRRETPSPTPSYGQQSTATLTPSPPDPPQ---PPTDMPHLHQMPRRVPLGPSSPL 1188

  Fly  1150 NQQQQLLLASNALKQGLGA------YANTTLAAQMAS--------GGGAGT-------LRRQRQP 1193
            :..|.:|....|...||||      :.:.:.|...||        |.|..|       .|.:::|
Human  1189 SVSQPMLGIREARPAGLGAGPAASPHLSPSPAPSTASSAPGRTWQGNGEMTPPLQGPRARFRKKP 1253

  Fly  1194 KTL-YKSENNILGKSGLRQNTMNASVGAPSNGQMDFLTGGPPSEGGDFSGLGLCNSTNQLLNDWA 1257
            |.| |:.||                    |.|.:......||.|..                .||
Human  1254 KALPYRREN--------------------SPGDLPPPPLPPPEEEA----------------SWA 1282

  Fly  1258 SSASIAAPGDYHFGSKQPSKQHLYVKA-----------KDGTWSAVSSDAYQSFKHQQQHHPFLA 1311
              ..:.|.|......::.|.:...|:|           .:..|...|..::.|           .
Human  1283 --LELRAAGSMSSLERERSGERKAVQAVPLAAQRVLHPDEEAWLPYSRPSFLS-----------R 1334

  Fly  1312 GSGDNTKSLASVNSLAGDSKFLSSFGS 1338
            |.|.:|.|.|..||..|.|   ||.||
Human  1335 GQGTSTCSTAGSNSSRGSS---SSRGS 1358

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	NP_608592.2	Ig	22..117	CDD:299845	51/97 (53%)
		I-set	23..116	CDD:254352	51/96 (53%)
		I-set	122..211	CDD:254352	38/89 (43%)
		Ig	125..211	CDD:299845	36/86 (42%)
		I-set	215..302	CDD:254352	36/86 (42%)
		Ig	232..302	CDD:299845	29/69 (42%)
		I-set	306..401	CDD:254352	27/96 (28%)
		Ig	320..411	CDD:299845	27/93 (29%)
		I-set	412..492	CDD:254352	29/80 (36%)
		IGc2	429..489	CDD:197706	19/60 (32%)
		FN3	519..612	CDD:238020	31/92 (34%)
		FN3	644..735	CDD:238020	20/93 (22%)
		fn3	743..832	CDD:278470	30/91 (33%)
ROBO3	NP_071765.2	Ig	64..162	CDD:299845	51/97 (53%)
		I-set	64..161	CDD:254352	51/96 (53%)
		I-set	168..249	CDD:254352	37/83 (45%)
		Ig	170..254	CDD:299845	36/86 (42%)
		I-set	258..343	CDD:254352	36/86 (42%)
		Ig	275..343	CDD:299845	29/69 (42%)
		I-set	347..441	CDD:254352	28/98 (29%)
		Ig	361..450	CDD:299845	27/93 (29%)
		I-set	451..531	CDD:254352	29/80 (36%)
		IGc2	465..528	CDD:197706	20/63 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	541..563		8/31 (26%)
		FN3	556..649	CDD:238020	32/99 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	639..662		10/28 (36%)
		FN3	680..760	CDD:238020	17/81 (21%)
		FN3	770..863	CDD:238020	32/98 (33%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	965..989		4/25 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1028..1310		72/346 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1327..1386		15/46 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6690
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D173520at2759
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm8568
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	10	9.680