DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and ROBO1

DIOPT Version :9

Sequence 1:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster
Sequence 2:	XP_011532278.1	Gene:	ROBO1 / 6091	HGNCID:	10249	Length:	1654	Species:	Homo sapiens

Alignment Length:	1496	Identity:	438/1496 - (29%)
Similarity:	648/1496 - (43%)	Gaps:	292/1496 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 QGSHPPRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPL---KILPGSHRITLPAGGL 79
            |...||||||||.|..|.:.|||||||||||.|||||:|||.|..:   |..|.|||:.||:|.|
Human    63 QEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSL 127

  Fly    80 FFLKVVNSRR-ETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECA 143
            |||::|:.|: ..|.|:|.|.|:|.||.|.|.||:|:||:|||:||..|.:..:|.|:.|::||.
Human   128 FFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQ 192

  Fly   144 APRGIPEPTVTWKKGGQKLDLEGSKRVRI-VDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLA 207
            .|||.||||::|||.|..||   .|..|| :.||.|.|...|::|.|:|.|:..|.||.|||.:|
Human   193 PPRGHPEPTISWKKDGSPLD---DKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVA 254

  Fly   208 TLKVHVKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLEDRSLRL 272
            .|.|..:|..::.|.:..|....|..|.|...|||:|.|.|.:  ..|.:|..|..:.:|.:|::
Human   255 ELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRK--DDGELPKSRYEIRDDHTLKI 317

  Fly   273 ERVTIADEGEYSCEADNVVGAITAMGTLTVYA---PPKFIQRPASKSVELGADTSFECRAIGNPK 334
            .:||..|.|.|:|.|:|:||...|..||||..   ||.|:.:|..:.|.||...:|:|.|.|||:
Human   318 RKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQ 382

  Fly   335 PTIFWTIKNNSTLIFPGAPP--LDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITS 397
            |.|||..:.:..|:|...||  ..||....|.:    ||:|..||:|....| |..:|...||.:
Human   383 PAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGD----LTITNVQRSDVGYYI-CQTLNVAGSIIT 442

  Fly   398 RVQLSL-DSQEDRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQ-PSSKLNI 460
            :..|.: |...|||||:|..||||||:.:.....|.|.|.|.|.|||.|.:||:.|. ..|::..
Human   443 KAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQ 507

  Fly   461 TTSGDLIISDLDRQQDQGLYTCVASSRAGKSTWSGFLRIE---------LPTNPNIKFYRAPEQT 516
            ..:|.|.|. ..:..|.|.|||:||:.:|::|||.::.::         .||:||:
Human   508 LENGVLQIR-YAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNL--------- 562

  Fly   517 KCPSAPGQPKILNATASALTIVWPTSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVE 581
             .||||.:|::.:.:.:.:|:.|..:..:||:. ..|.:|.:......:|..:|..:......::
Human   563 -IPSAPSKPEVTDVSRNTVTLSWQPNLNSGATP-TSYIIEAFSHASGSSWQTVAENVKTETSAIK 625

  Fly   582 SLTQGAAYMFIVRAENSLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVV 646
            .|...|.|:|:|||.|:.|.|.||.||:|:....::....|.:.....:.|         .|.|:
Human   626 GLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQREL---------GNAVL 681

  Fly   647 ELLEANASDSTTARLSWDID-SGQYIEGFYLYARE--LHSSEYKMVTLLNKGQGLSSCTVPGLAK 708
            .|.......|::..:.|.:| ..|||:|:.:..|.  .:..|...:....:....:|..:|.|.|
Human   682 HLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRK 746

  Fly   709 ASTYEFFLVPFYKSIVGKPSNSRRMRTLEDVPEAPPYGMEAIQ--FNRTSVFLKWLPPQPNRTRN 771
            ...||....||:....|..|..:..:|||:.|.|||.|:...:  .|.|::.:.|.|| |..|:|
Human   747 GVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPP-PEDTQN 810

  Fly   772 GILTSYNVVVKGLDVHNTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAV 836
            |::..|.|...|    |.||...|.|:|.:..::::..|..|:.|.:.|||:|..|.|..|:|..
Human   811 GMVQEYKVWCLG----NETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQF 871

  Fly   837 LRIDARTQSLDTGYTRYPISRDIADDFLTQTWFIVLLGSIIAIIVFLLGALVLFKRYQFIKQTSL 901
            :::||....: :...:..:::.|: |.:.|..||..:|:...||:.:. ::.|::..:  |:..|
Human   872 IQLDAHGNPV-SPEDQVSLAQQIS-DVVKQPAFIAGIGAACWIILMVF-SIWLYRHRK--KRNGL 931

  Fly   902 GSLHGNHAIGTVRKFPTLPL-------NGGGAVGAGGS----NSTAAAGQSGHGLWIDPTSGVWR 955
            .|.:..     :||.|:...       .||.||.:||.    |.:..|.|.    |:..|   |.
Human   932 TSTYAG-----IRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQP----WLADT---WP 984

  Fly   956 QAAGGGNPGGVGSCTTKEQLPGYAQATAQQGQQPTLLPDYER---------------LSPLNMPD 1005
            ......|...:..|            ||..|...:.|..|.|               .:.|.:|:
Human   985 NTGNNHNDCSISCC------------TAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPE 1037

  Fly  1006 ---YAEVACS-------TFKSPT---------HGAPP---------------MGGGGGGQSASLY 1036
               |.:|..|       ||.||.         .|.|.               |..|.|......:
Human  1038 STVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHW 1102

  Fly  1037 DSCG------AYATTNVVANVKLYQNRYA--TKP-----NQS-----SGSNNNNHQGNDYQST-- 1081
            ...|      |....|:|...||.::..|  |.|     |||     .||.|::.:|:....:
Human  1103 KPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQG 1167

  Fly  1082 --------------GMYSA----PPSAH---------YG------------CLEPKQQQQQQQ-- 1105
                          ||..|    ||.||         |.            |..|..:...||
Human  1168 HKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDE 1232

  Fly  1106 --------------------PNLMTTSTASTAILTNSPAKVKKINITENKMDQLEGKSERTNPFN 1150
                                |..::.|..|||.||.||        .|.....|:...|.|....
Human  1233 LEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSP--------QEELQPMLQDCPEETGHMQ 1289

  Fly  1151 QQQQLLLASNALKQGLG--------------AYANTTLAAQMASGGGAGTLRRQRQPKTLYKSEN 1201
            .|      .:..:|.:.              .|.:..|.:.|.:  .|...........:.|.:.
Human  1290 HQ------PDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDT--DAPEEEEDEADMEVAKMQT 1346

  Fly  1202 NILGKSGLRQNTMNASVG---APSNGQMDFLTG-GPPSEGGDFSG--LGLCNSTNQLLNDWASSA 1260
            ..|...||.| |..:|||   :...|.|  :.| |..||..:.|.  ..:.:|......|...:.
Human  1347 RRLLLRGLEQ-TPASSVGDLESSVTGSM--INGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQ 1408

  Fly  1261 SIAAPGDYHFGSKQPSKQ--------HLYVKAKDGTWSAVSSDAYQS------------FKHQQQ 1305
            ::||..:| .|.|...:|        |.:........|.||:|:..|            .|||..
Human  1409 AVAAAAEY-AGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPG 1472

  Fly  1306 H 1306
            |
Human  1473 H 1473

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	NP_608592.2	Ig	22..117	CDD:299845	57/98 (58%)
		I-set	23..116	CDD:254352	56/96 (58%)
		I-set	122..211	CDD:254352	41/89 (46%)
		Ig	125..211	CDD:299845	39/86 (45%)
		I-set	215..302	CDD:254352	30/86 (35%)
		Ig	232..302	CDD:299845	26/69 (38%)
		I-set	306..401	CDD:254352	34/96 (35%)
		Ig	320..411	CDD:299845	33/93 (35%)
		I-set	412..492	CDD:254352	32/80 (40%)
		IGc2	429..489	CDD:197706	23/60 (38%)
		FN3	519..612	CDD:238020	28/92 (30%)
		FN3	644..735	CDD:238020	21/93 (23%)
		fn3	743..832	CDD:278470	30/90 (33%)
ROBO1	XP_011532278.1	Ig1_Robo	67..166	CDD:143317	57/98 (58%)
		I-set	68..165	CDD:254352	56/96 (58%)
		I-set	172..258	CDD:254352	41/88 (47%)
		Ig2_Robo	174..258	CDD:143201	39/86 (45%)
		I-set	262..347	CDD:254352	30/86 (35%)
		Ig3_Robo	279..347	CDD:143202	26/69 (38%)
		I-set	354..448	CDD:254352	35/98 (36%)
		Ig4_Robo	368..457	CDD:143203	33/93 (35%)
		I-set	458..545	CDD:254352	35/87 (40%)
		Ig	462..536	CDD:299845	29/74 (39%)
		FN3	564..650	CDD:238020	24/86 (28%)
		FN3	687..773	CDD:238020	19/85 (22%)
		fn3	781..867	CDD:278470	30/90 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6690
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2206
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm8568
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	10	9.670