DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and ROBO4

DIOPT Version :9

Sequence 1:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster
Sequence 2:	NP_061928.4	Gene:	ROBO4 / 54538	HGNCID:	17985	Length:	1007	Species:	Homo sapiens

Alignment Length:	1050	Identity:	237/1050 - (22%)
Similarity:	349/1050 - (33%)	Gaps:	378/1050 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 IVFLLKWTHAQGSHPPRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPLKILPGSHRI 72
            ::.|:....||.| ||:|:.||.|.......||.::|:|.|.|.|||:|..:|.||.::|.....
Human    18 LLLLIMGGMAQDS-PPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHH 81

  Fly    73 TLPAGGLFFLKVV-------NSRRETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNT 130
            .||.|.|..|:..       .....||.|:|.|||.|.||.|.||.|.|.|||||::|:::|::.
Human    82 LLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDM 146

  Fly   131 RIAQGDTALLECAAPRGIPEPTVTWKKGGQKLDLEGSKRVRIVDGGNLAIQDARQTDEGQYQCIA 195
            ....|:...|||..|.|.|||||:|.|.|:.|.|:..:..  |.||:|.:..|.::|||.|.|:|
Human   147 VAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHT--VSGGSLLMARAEKSDEGTYMCVA 209

  Fly   196 KNPVGVRESSLATLKVHVKPYIIRGPHDQT----------VLEGASVTFPCRVGGDPMPDVLWL- 249
            .|..|.|||..|.:.       |:.|.|.|          .||..::..|....|......:||
Human   210 TNSAGHRESRAARVS-------IQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLS 267

  Fly   250 -------------------RTASGG--------------NMPLDRVSVLEDRS------------ 269
                               :||.||              :..|..:...:|..
Human   268 WKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARG 332

  Fly   270 -------LRL-ERVTIADEGEYSCEADNVVGAITAMGTLTV-YAPPKFIQRPASKSVELGADTSF 325
                   ||| |:|..|...|.:.:..|        ||:.| :.||     ||..  ..|....:
Human   333 PDSNVLLLRLPEKVPSAPPQEVTLKPGN--------GTVFVSWVPP-----PAEN--HNGIIRGY 382

  Fly   326 ECRAIGNPK-PTIFWTIKNNSTLI-----FPGA---------------PP------LDRFHSLNT 363
            :..::||.. |...||:....|.:     .||:               |.      |::.....|
Human   383 QVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERAT 447

  Fly   364 EE--GHSILTLTRFQRTDK--DLVILCNA------------------------------------ 388
            :|  .|...||.:.:.|.|  :::..|..
Human   448 QEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAI 512

  Fly   389 -------------------------MNEVASITSRV-------------QLSLDSQED------- 408
                                     ::..:|::||:             .||.||:..
Human   513 LKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPD 577

  Fly   409 ------------------RPPPIIISGPVNQTLPIKSLATLQ---------CKAIGLPSPTIS-- 444
                              ||.|.:   |..:.|| ..||.|.         |...||.||.:|
Human   578 TSTFYGSLIAELPSSTPARPSPQV---PAVRRLP-PQLAQLSSPCSSSDSLCSRRGLSSPRLSLA 638

  Fly   445 ----WYRDGIPVQPSSKLNITTSGDLIISDLDRQQDQGLYTCVASSRAGKS-------------T 492
                |     ..:...:|....|..|    |.......|..|...:|..|:             .
Human   639 PAEAW-----KAKKKQELQHANSSPL----LRGSHSLELRACELGNRGSKNLSQSPGAVPQALVA 694

  Fly   493 W-----------SGFLRIELPTNPNIKFYRAP---EQTKCPSAPGQPKILNATASALTIVWPTSD 543
            |           :..:...||..|.......|   :||:.|.||..|..:...|:.:.|:.|.|.
Human   695 WRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPCSP 759

  Fly   544 KA-GASSFLGYSVEMYCTNQSRTWIPIASRLS-----------EPIFTVE------SLTQGAAYM 590
            .: .|||..|.|             |.:||||           :.:.|.|      .|::|..
Human   760 PSPQASSLSGPS-------------PASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEE-- 809

  Fly   591 FIVRAENSLGFSPPSPISEPITAGKLVGVRDGSESTGTSQ----------LLLSDVETLLQ---- 641
               ...||:...|.:| |.|.|.| .:.|...||.|...:          :||......|.
Human   810 ---TPRNSVSPMPRAP-SPPTTYG-YISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPS 869

  Fly   642 ----ANDVVELLEANASDSTTARLSWDIDSGQYIEGFYLYARELHSSEYKMVTLLNKGQGL---- 698
                ||......|.||:.:..:.:|..       :|.:|  .:.|.:....|.:.:.|.||
Human   870 EGSLANGWGSASEDNAASARASLVSSS-------DGSFL--ADAHFARALAVAVDSFGFGLEPRE 925

  Fly   699 SSCTVPGLAKAST-----YEFFLVPFYKSIVGKPSNSRRMRTLEDVP--EAPPYGMEAIQFNRTS 756
            :.|.   ...||:     .|.||.|..                 .:|  |..|..:|.::.:.|.
Human   926 ADCV---FIDASSPPSPRDEIFLTPNL-----------------SLPLWEWRPDWLEDMEVSHTQ 970

  Fly   757 VFLKWLPPQP 766
            ...:.:||.|
Human   971 RLGRGMPPWP 980

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	NP_608592.2	Ig	22..117	CDD:299845	40/101 (40%)
		I-set	23..116	CDD:254352	39/99 (39%)
		I-set	122..211	CDD:254352	35/88 (40%)
		Ig	125..211	CDD:299845	34/85 (40%)
		I-set	215..302	CDD:254352	26/150 (17%)
		Ig	232..302	CDD:299845	20/123 (16%)
		I-set	306..401	CDD:254352	25/199 (13%)
		Ig	320..411	CDD:299845	27/220 (12%)
		I-set	412..492	CDD:254352	22/107 (21%)
		IGc2	429..489	CDD:197706	16/74 (22%)
		FN3	519..612	CDD:238020	28/110 (25%)
		FN3	644..735	CDD:238020	18/99 (18%)
		fn3	743..832	CDD:278470	5/23 (22%)
ROBO4	NP_061928.4	Ig	31..133	CDD:299845	40/101 (40%)
		I-set	32..132	CDD:254352	39/99 (39%)
		I-set	139..220	CDD:254352	34/82 (41%)
		Ig2_Robo	141..225	CDD:143201	34/92 (37%)
		fn3	350..432	CDD:278470	18/96 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	531..551		3/19 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	713..793		24/92 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	806..826		6/25 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	971..1007		2/9 (22%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	81	1.000	Domainoid score	I8493
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	3	2.860