DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and robo1

DIOPT Version :9

Sequence 1:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster
Sequence 2:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster

Alignment Length:	1397	Identity:	455/1397 - (32%)
Similarity:	666/1397 - (47%)	Gaps:	255/1397 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    23 PRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPLKI-LPGSHRITLPAGGLFFLKVVN 86
            |||:|||.|..|.::||||||||.||.|.|||:|:|||.|:.. ...|||:....|.|||.:.:.
  Fly    56 PRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGEPVSTNEKKSHRVQFKDGALFFYRTMQ 120

  Fly    87 SRRETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECAAPRGIPEP 151
            .::|.|.|.|||.|||.:|.|.||:|:||:|||||:||:||::||:|:|:||||||..|:|||||
  Fly   121 GKKEQDGGEYWCVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGIPEP 185

  Fly   152 TVTWKKGGQKLD------LEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLATLK 210
            |:.|.|.|..||      ...|.||||||||||.|.:....|||.|:|||:|.||.||||.|.|.
  Fly   186 TLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYAKLI 250

  Fly   211 VHVKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLED-RSLRLER 274
            |.||||.::.|.||.:|.|.:.||.|.|||||.|.|||.:  ..||:|:.|..:|.| :||.:..
  Fly   251 VQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKK--EEGNIPVSRARILHDEKSLEISN 313

  Fly   275 VTIADEGEYSCEADNVVGAITAMGTLTVYAPPKFIQRPASKSVELGADTSFECRAIGNPKPTIFW 339
            :|..|||.|.|||.|.||.|:|..:|.|:|||.|.:||::|.|.|.......|.|.|||.|::||
  Fly   314 ITPTDEGTYVCEAHNNVGQISARASLIVHAPPNFTKRPSNKKVGLNGVVQLPCMASGNPPPSVFW 378

  Fly   340 TIKNNSTLIFPGAPPLDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITSRVQLSLD 404
            |.:..|||:||.:.     |..........|.:|..::.|:...: |:|.:.|.|.|.||.|.:.
  Fly   379 TKEGVSTLMFPNSS-----HGRQHVAADGTLQITDVRQEDEGYYV-CSAFSVVDSSTVRVFLQVS 437

  Fly   405 SQEDRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQPSSKLNITTSGDLIIS 469
            |.::||||||..||.|||||..|:|||.|:|.|.|||.|.|:.||..||..::.:|.....|.:.
  Fly   438 SLDERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRIKWFHDGHAVQAGNRYSIIQGSSLRVD 502

  Fly   470 DLDRQQDQGLYTCVASSRAGKSTWSGFLRIELPTNPNIKFYRAPEQTKCPSAPGQPKILNATASA 534
            || :..|.|.|||.||...|:::|:..|.:|.|.:.::  :||.:.:..|:.||.||:||.:.::
  Fly   503 DL-QLSDSGTYTCTASGERGETSWAATLTVEKPGSTSL--HRAADPSTYPAPPGTPKVLNVSRTS 564

  Fly   535 LTIVWPTS-DKAGA-SSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVESLTQGAAYMFIVRAEN 597
            :::.|..| :|.|| ...:||:||.:..:....||..|.|:.:...|:..||.|.:|:|:|||||
  Fly   565 ISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAEN 629

  Fly   598 SLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVVELLEANASDSTTARLS 662
            :.|.|.||.:|..|   |.:.....:.|...    ||...|||.... |||::|:|.:::..||.
  Fly   630 TQGISVPSGLSNTI---KTIEADFDAASAND----LSAARTLLTGKS-VELIDASAINASAVRLE 686

  Fly   663 WDID---SGQYIEGFYLYARE--LHSSEYKMVTLLNKGQGLSSCTVPGLAKASTYEFFLVPFYKS 722
            |.:.   ..:|:||..::.::  :.|::|..:|:::  ....|..|..|.|.:.|||||.||:::
  Fly   687 WMLHVSADEKYVEGLRIHYKDASVPSAQYHSITVMD--ASAESFVVGNLKKYTKYEFFLTPFFET 749

  Fly   723 IVGKPSNSRRMRTLEDVPEAPPYGMEAIQFNRTSVFLKWLPPQPNRTRNGILTSYNVVVKGLDVH 787
            |.|:||||:...|.||||.|||..::...:|:|:.:::|.|| |::..||.|..|.:.|   ...
  Fly   750 IEGQPSNSKTALTYEDVPSAPPDNIQIGMYNQTAGWVRWTPP-PSQHHNGNLYGYKIEV---SAG 810

  Fly   788 NTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAVLRI------------- 839
            ||.::..|||::|...::||.|||||..|.:.:.:.|:.|.||:|||..|.:
  Fly   811 NTMKVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTHHVHPPRAHP 875

  Fly   840 ----DARTQSLDTGYTR---------YPISRDIADDFLTQTWFIVLLGSIIAIIVFLLGA---LV 888
                |.|.:..|..|..         .|.:.....|:|:..|.:||:  .|.::|.::.|   :|
  Fly   876 SGTHDGRHEGQDLTYHNNGNIPPGDINPTTHKKTTDYLSGPWLMVLV--CIVLLVLVISAAISMV 938

  Fly   889 LFKR-YQFIKQTSLGSLHGNHAIGTVRKFPTLPLNGGGAVGAGGSNSTAAAGQSGHGLWIDPTSG 952
            .||| :|..|:  ||.|    ::.:..:...|.:|                  |...||||...|
  Fly   939 YFKRKHQMTKE--LGHL----SVVSDNEITALNIN------------------SKESLWIDHHRG 979

  Fly   953 VWRQA--------------------------AGGG----------------------NPG----- 964
             ||.|                          :.||                      ||.
  Fly   980 -WRTADTDKDSGLSESKLLSHVNSSQSNYNNSDGGTDYAEVDTRNLTTFYNCRKSPDNPTPYATT 1043

  Fly   965 ---GVGS---CTTKEQLPG---------YAQATAQQGQQPTLLPDYERLSPLNMPDYAE-----V 1009
               |..|   ||....:..         |:.|.|  ||.|.:        |:...:|.:     :
  Fly  1044 MIIGTSSSETCTKTTSISADKDSGTHSPYSDAFA--GQVPAV--------PVVKSNYLQYPVEPI 1098

  Fly  1010 ACSTFKSPTHGAPPMGG------GGGGQSASLYDSCGAYATTN-VVANVKLY------------- 1054
            ..|.|..|....||...      |....|.....|.|:..:|| .:.|..::
  Fly  1099 NWSEFLPPPPEHPPPSSTYGYAQGSPESSRKSSKSAGSGISTNQSILNASIHSSSSGGFSAWGVS 1163

  Fly  1055 ---------QNRYATKPNQSSGSNNNNHQGND-----------YQSTGMYSAPPSAHYGCLEPKQ 1099
                     :|.|:...:..:|...|.:|...           :.:||...|.|..|......:.
  Fly  1164 PQYAVACPPENVYSNPLSAVAGGTQNRYQITPTNQHPPQLPAYFATTGPGGAVPPNHLPFATQRH 1228

  Fly  1100 QQQQQQPNLMTTSTAST-------AILTNSPAK-VKKINITENKMDQLEGKSERTNPFNQQQQLL 1156
            ...:.|..|.....|.:       |:.|.||.: ...:.:.|.....:...|...:|.:...|:.
  Fly  1229 AASEYQAGLNAARCAQSRACNSCDALATPSPMQPPPPVPVPEGWYQPVHPNSHPMHPTSSNHQIY 1293

  Fly  1157 LASNALKQGLGAYANTTLAAQMASGGGAGTLR----RQRQP--KTLYKSEN-NILGKSGLRQNT- 1213
            ..|:.......:..:.....|:...|.:...|    |:|.|  :...:||| |:|.:...||.|
  Fly  1294 QCSSECSDHSRSSQSHKRQLQLEEHGSSAKQRGGHHRRRAPVVQPCMESENENMLAEYEQRQYTS 1358

  Fly  1214 -------------------MNASVGAPSNGQM 1226
                               :.|..|.|:..||
  Fly  1359 DCCNSSREGDTCSCSEGSCLYAEAGEPAPRQM 1390

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	NP_608592.2	Ig	22..117	CDD:299845	50/94 (53%)
		I-set	23..116	CDD:254352	49/93 (53%)
		I-set	122..211	CDD:254352	54/94 (57%)
		Ig	125..211	CDD:299845	52/91 (57%)
		I-set	215..302	CDD:254352	41/87 (47%)
		Ig	232..302	CDD:299845	34/70 (49%)
		I-set	306..401	CDD:254352	32/94 (34%)
		Ig	320..411	CDD:299845	28/90 (31%)
		I-set	412..492	CDD:254352	38/79 (48%)
		IGc2	429..489	CDD:197706	26/59 (44%)
		FN3	519..612	CDD:238020	36/94 (38%)
		FN3	644..735	CDD:238020	31/95 (33%)
		fn3	743..832	CDD:278470	31/88 (35%)
robo1	NP_476899.1	Ig	56..151	CDD:299845	50/94 (53%)
		I-set	56..150	CDD:254352	49/93 (53%)
		I-set	157..251	CDD:254352	54/93 (58%)
		Ig2_Robo	159..251	CDD:143201	52/91 (57%)
		I-set	255..341	CDD:254352	41/87 (47%)
		Ig3_Robo	272..341	CDD:143202	34/70 (49%)
		IG_like	351..436	CDD:214653	30/90 (33%)
		Ig	362..444	CDD:299845	28/87 (32%)
		I-set	445..531	CDD:254352	40/86 (47%)
		IGc2	459..521	CDD:197706	27/62 (44%)
		FN3	549..637	CDD:238020	33/87 (38%)
		FN3	673..762	CDD:238020	28/90 (31%)
		fn3	770..855	CDD:278470	31/88 (35%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6635
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0.917438	Normalized mean entropy	S5483
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D173520at2759
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm6572
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
	8	7.770