DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and robo1

DIOPT Version :9

Sequence 1:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster
Sequence 2:	XP_017214701.2	Gene:	robo1 / 30769	ZFINID:	ZDB-GENE-000209-3	Length:	1705	Species:	Danio rerio

Alignment Length:	1395	Identity:	425/1395 - (30%)
Similarity:	634/1395 - (45%)	Gaps:	253/1395 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 QGSHPPRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPLKI---LPGSHRITLPAGGL 79
            |..:.|||||||.|..|.:.|||||||||||.||||::|||||..::.   .|.|||:.||.|.|
Zfish    80 QEDYAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDRDNPRSHRMLLPTGSL 144

  Fly    80 FFLKVVNSRR-ETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECA 143
            |||::|:.|| :.|.|.|.|.|:|.||.|.|.||:|:||:|||:||..|.:..:|.|:.|::||.
Zfish   145 FFLRIVHGRRSKPDEGSYVCVARNYLGEAVSHNASLEVAILRDDFRQNPVDVMVAAGEPAVMECQ 209

  Fly   144 APRGIPEPTVTWKKGGQKLDLEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLAT 208
            .|||.||||::|||.|..:| :..:|:.| .||.|.|.:.|:||.|:|.|:..|.:|.|||.:|.
Zfish   210 PPRGHPEPTISWKKDGVNID-DRDERITI-RGGKLMITNTRKTDAGKYVCVGTNMMGERESEIAE 272

  Fly   209 LKVHVKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLEDRSLRLE 273
            |.|..:|..:|.|..|:||...||.|.|...|||:|.:.|.:  ..|.:|..|..:.||.:|:|:
Zfish   273 LTVLERPSFLRKPSSQSVLVDQSVEFRCEAQGDPVPTIRWRK--EDGELPKGRYEIWEDHTLKLK 335

  Fly   274 RVTIADEGEYSCEADNVVGAITAMGTLTVY---APPKFIQRPASKSVELGADTSFECRAIGNPKP 335
            |:|..|.|.|:|:|:|::|...|..||||:   .||.|:.||.::...:|...:|:|.|.|:|:|
Zfish   336 RLTSGDAGTYTCQAENMMGKTEASATLTVHVVSVPPVFVVRPRNQVAGMGRTVTFQCEATGSPQP 400

  Fly   336 TIFWTIKNNSTLIFPG--APPLDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITSR 398
            .|||..:.:..|:|..  .||..||....|.:    ||:|..:|:|.. ...|.|:|...|:.::
Zfish   401 AIFWQKEGSQNLLFSSQPPPPSSRFSVSQTGD----LTITAVERSDGG-YYSCQALNIAGSVITK 460

  Fly   399 VQLSL-DSQEDRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQP-SSKLNIT 461
            ..|.: |...||||||:..||.|||:.:.....|.|:|.|.|.|||.|.:||:.|.. .|:|...
Zfish   461 ALLEVTDVISDRPPPIVRQGPTNQTVAVDGTVVLSCQATGTPIPTILWRKDGVLVSTHDSRLKQL 525

  Fly   462 TSGDLIISDLDRQQDQGLYTCVASSRAGKSTWSGFLRIE---------LPTNPNIKFYRAPEQTK 517
            .:|.|.|. ..:..|.|:|||.||:.:|:::|..:|.::         .||:||:
Zfish   526 DTGALQIR-YAKLGDTGMYTCTASTPSGEASWKAYLEVQEFGAPVQPGRPTDPNL---------- 579

  Fly   518 CPSAPGQPKILNATASALTIVWPTSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVES 582
            .||||.:|.:.:.:.:::::.|..:..|||:. ..|.:|.:......:|..:|..:....|.::.
Zfish   580 IPSAPSKPDVTDVSRTSVSLSWKPNLNAGATP-TSYVIEAFSHAAGSSWQTLADHVKTESFVLKG 643

  Fly   583 LTQGAAYMFIVRAENSLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVVE 647
            |...|.|:|:|||.|:.|.|.|||||:.:....:.....|.|. |..|..|.||        |:.
Zfish   644 LKPSAVYLFLVRAANAYGLSDPSPISDAVKTQDVPPTSQGVEH-GQIQRELGDV--------VIH 699

  Fly   648 LLEANASDSTTARLSWDID-SGQYIEGFYLYARELHSSEYKMV--TLLNKG-QGLSSCTVPGLAK 708
            |.......|::.|:.|.:: ..|||:|:.:..|.......:.|  .:...| .|..|..|..|.|
Zfish   700 LHNPTILSSSSVRVQWTVEQQSQYIQGYKVMYRPSPEGAPQRVDWAMFEVGAPGEHSAVVTQLKK 764

  Fly   709 ASTYEFFLVPFYKSIVGKPSNSRRMRTLEDVPEAPPYGMEAIQF--NRTSVFLKWLPPQPNRTRN 771
            ..||||.:.||:....|..|:.:..:|||:.|.|.|.|:.....  |.|:|.:.|.|| |...:|
Zfish   765 GITYEFKVRPFFNEFQGTDSDIKVGKTLEEAPSAAPRGVTVTGSGDNGTAVLVAWQPP-PEEEQN 828

  Fly   772 GILTSYNVVVKGLDVHNTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAV 836
            |::..|.:...|    |.:|...|.|:|.:..::|:..|..||||.:.|||.|..|.|..|.|..
Zfish   829 GVVQEYKIWCLG----NESRYHINRTVDGSTHSVLIPGLVAGVTYRLEVAAGTGAGPGVKSDPTS 889

  Fly   837 LRIDARTQ----SLDTGYTRYPISRDIADDFLTQTWFIVLLGSIIAIIVFLLGALVLFKRYQFIK 897
            .::|:..:    .||...|   ||:.|: |.:.|..||..:|:...||:.:. ::.|::..:  |
Zfish   890 FQLDSSGRVLPPPLDDDNT---ISQQIS-DVVRQPAFIAGIGAACWIILMVF-SIWLYRHRK--K 947

  Fly   898 QTSLGSLHGNHAIGTVRKFPTLPL-------NGGGAVGAGGSNSTAAAGQSGHGLWIDPTSGVWR 955
            :..|.|.:..     :||.|:...       .||.||.:.|.......|:.....|:..|   |.
Zfish   948 RNGLSSSYAG-----IRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNIGEPATQPWLADT---WP 1004

  Fly   956 QAAGGGNPGGVGSCTTKEQLPGYAQATAQQGQQPTLLPDYER---------------LSPLNMPD 1005
            .:....|...:..|            |...|...:.|..|.|               .:.|..||
Zfish  1005 NSCSNHNDCTINCC------------TGSNGNSDSNLATYSRPADCIANYSNQMDNKATNLLAPD 1057

  Fly  1006 ---YAEVACS-------TFKSPTHGAPPMGGGGGGQSASLYDSCGAYATTNVVANVKLYQNRYAT 1060
               |::|..|       ||.||........|.||..:        .||||.::
Zfish  1058 TGLYSDVDLSNKINEMKTFNSPNLKDGRFMGPGGQPT--------PYATTQLI------------ 1102

  Fly  1061 KPNQSSGSNNNNHQGNDYQSTGMYSAPPSAHYGCLEPKQQQQQQQPNLMTTSTASTAILTNSPAK 1125
               |||.:|||.:..:.....|      ||.:   ..||..:..|               .:|.|
Zfish  1103 ---QSSLANNNMNSSSGGSGGG------SADF---NEKQSWKSSQ---------------GTPQK 1140

  Fly  1126 VKKIN-------ITENKMD----QLEGKSERTNPFNQQQQLLLASNALKQGLGAYANTTLAAQMA 1179
            ...||       :.:||::    :.||....|.|:||.|.:|..........|   ::|..:|..
Zfish  1141 QADINTPLQYNVMEQNKLNKDHYRGEGPISVTIPYNQAQDVLTGETYSSSDRG---SSTSGSQGQ 1202

  Fly  1180 SGGGAGTLRRQRQPKTLYKSENNILGKSGLRQNTMNASVGAPSNGQMDFLTGGPPSEGGDFSGLG 1244
            ..||    |..:|.|                ||.||.:...|...     ...||
Zfish  1203 KKGG----RTTKQAK----------------QNPMNWTELLPPPP-----VNPPP---------- 1232

  Fly  1245 LCNSTNQLLNDWASSASIAAPGDYHFGSKQPSKQHLY----------VKAKDG----TWSAVSSD 1295
             |.....:::| :....:..|       ..||:.:|.          |:.:.|    ...|.||.
Zfish  1233 -CQEYTLVMDD-SYDTDLQCP-------LPPSRMYLQPDELEEEEDEVELERGPTPPVRGAASSP 1288

  Fly  1296 AYQSFKH----------QQQHHPFLAGSGD 1315
            |..|:.|          |::.||.|..:.|
Zfish  1289 AAVSYSHQSTATLTPSPQEEMHPMLQDNPD 1318

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	NP_608592.2	Ig	22..117	CDD:299845	56/98 (57%)
		I-set	23..116	CDD:254352	56/96 (58%)
		I-set	122..211	CDD:254352	39/88 (44%)
		Ig	125..211	CDD:299845	37/85 (44%)
		I-set	215..302	CDD:254352	34/86 (40%)
		Ig	232..302	CDD:299845	27/69 (39%)
		I-set	306..401	CDD:254352	30/96 (31%)
		Ig	320..411	CDD:299845	30/93 (32%)
		I-set	412..492	CDD:254352	32/80 (40%)
		IGc2	429..489	CDD:197706	24/60 (40%)
		FN3	519..612	CDD:238020	29/92 (32%)
		FN3	644..735	CDD:238020	25/94 (27%)
		fn3	743..832	CDD:278470	30/90 (33%)
robo1	XP_017214701.2	Ig1_Robo	84..183	CDD:143317	56/98 (57%)
		Ig	191..275	CDD:325142	37/85 (44%)
		Ig3_Robo	296..363	CDD:143202	26/68 (38%)
		Ig	385..474	CDD:325142	30/93 (32%)
		Ig_3	474..548	CDD:316449	30/74 (41%)
		FN3	581..672	CDD:238020	29/91 (32%)
		FN3	704..791	CDD:238020	23/86 (27%)
		fn3	799..885	CDD:306538	30/90 (33%)
		Herpes_ICP4_C	1491..>1653	CDD:332854
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6635
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2206
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm6572
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	8	7.810