DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and Robo1

DIOPT Version :9

Sequence 1:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster
Sequence 2:	XP_017172382.1	Gene:	Robo1 / 19876	MGIID:	1274781	Length:	1687	Species:	Mus musculus

Alignment Length:	1291	Identity:	391/1291 - (30%)
Similarity:	589/1291 - (45%)	Gaps:	236/1291 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 QGSHPPRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPL---KILPGSHRITLPAGGL 79
            |...||||||||.|..|.:.|||||||||||.|||||:|||.|..:   |..|.|||:.||:|.|
Mouse    96 QEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSL 160

  Fly    80 FFLKVVNSRR-ETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECA 143
            |||::|:.|: ..|.|:|.|.|:|.||.|.|.||:|:||:|||:||..|.:..:|.|:.|::||.
Mouse   161 FFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQ 225

  Fly   144 APRGIPEPTVTWKKGGQKLDLEGSKRVRI-VDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLA 207
            .|||.||||::|||.|..||   .|..|| :.||.|.|...|::|.|:|.|:..|.||.|||.:|
Mouse   226 PPRGHPEPTISWKKDGSPLD---DKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVA 287

  Fly   208 TLKVHVKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLEDRSLRL 272
            .|.|..:|..::.|.:..|....|..|.|...|||:|.|.|.:  ..|.:|..|..:.:|.:|::
Mouse   288 ELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRK--DDGELPKSRYEIRDDHTLKI 350

  Fly   273 ERVTIADEGEYSCEADNVVGAITAMGTLTVYA---PPKFIQRPASKSVELGADTSFECRAIGNPK 334
            .:||..|.|.|:|.|:|:||...|..||||..   ||.|:.:|..:.|.||...:|:|.|.|||:
Mouse   351 RKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQ 415

  Fly   335 PTIFWTIKNNSTLIFPGAPP--LDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITS 397
            |.|||..:.:..|:|...||  ..||....|.:    ||:|..||:|....| |..:|...||.:
Mouse   416 PAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGD----LTITNVQRSDVGYYI-CQTLNVAGSIIT 475

  Fly   398 RVQLSL-DSQEDRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQ-PSSKLNI 460
            :..|.: |...|||||:|..||||||:.:.....|.|.|.|.|:|||.|.:||:.|. ..|::..
Mouse   476 KAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQ 540

  Fly   461 TTSGDLIISDLDRQQDQGLYTCVASSRAGKSTWSGFLRIE---------LPTNPNIKFYRAPEQT 516
            ..||.|.|. ..:..|.|.|||.||:.:|::|||.::.::         .||:||:
Mouse   541 LESGVLQIR-YAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNL--------- 595

  Fly   517 KCPSAPGQPKILNATASALTIVWPTSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVE 581
             .||||.:|::.:.:.:.:|:.|..:..:||:. ..|.:|.:......:|...|..:....|.::
Mouse   596 -IPSAPSKPEVTDVSKNTVTLSWQPNLNSGATP-TSYIIEAFSHASGSSWQTAAENVKTETFAIK 658

  Fly   582 SLTQGAAYMFIVRAENSLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVV 646
            .|...|.|:|:|||.|:.|.|.||.||:|:....:.....|.:.....:.|         .|.|:
Mouse   659 GLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQREL---------GNVVL 714

  Fly   647 ELLEANASDSTTARLSWDID-SGQYIEGFYLYARELHSS--EYKMVTLLNKGQGLSSCTVPGLAK 708
            .|.......|::..:.|.:| ..|||:|:.:..|...:|  |.:.:....:....:|..:|.|.|
Mouse   715 HLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRK 779

  Fly   709 ASTYEFFLVPFYKSIVGKPSNSRRMRTLEDVPEAPPYGMEAIQ--FNRTSVFLKWLPPQPNRTRN 771
            ...||....||:....|..|..:..:|||:.|.|||..:...:  .|.|::.:.|.|| |..|:|
Mouse   780 GVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPP-PEDTQN 843

  Fly   772 GILTSYNVVVKGLDVHNTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAV 836
            |::..|.|...|    |.|:...|.|:|.:..::::.:|..|:.|.:.|||:|..|.|..|:|..
Mouse   844 GMVQEYKVWCLG----NETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQF 904

  Fly   837 LRIDARTQSLDTGYTRYPISRDIADDFLTQTWFIVLLGSIIAIIVFLLGALVLFKRYQFIKQTSL 901
            :::|:....: :...:..:::.|: |.:.|..||..:|:...||:.:. ::.|::..:  |:..|
Mouse   905 IQLDSHGNPV-SPEDQVSLAQQIS-DVVRQPAFIAGIGAACWIILMVF-SIWLYRHRK--KRNGL 964

  Fly   902 GSLHGNHAIGTVRKFPTLPL-------NGGGAVGAGGSNSTAAAGQSGHGLWIDPTSGVWRQAAG 959
            .|.:..     :||.|:...       .||.||.:||........:.....|:..|   |.....
Mouse   965 TSTYAG-----IRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADT---WPNTGN 1021

  Fly   960 GGNPGGVGSCTTKEQLPGYAQATAQQGQQPTLLPDYER---------------LSPLNMPD---Y 1006
            ..|...:..|            ||..|...:.|..|.|               .:.|.:|:   |
Mouse  1022 NHNDCSINCC------------TAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVY 1074

  Fly  1007 AEVACS-------TFKSPT---------HGAPPMGGGGGGQSASLYDSCGAYATTNVVANVKLYQ 1055
            .:|..|       ||.||.         .|.|.                 .||||.::
Mouse  1075 GDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPT-----------------PYATTQLI------- 1115

  Fly  1056 NRYATKPNQSSGSNNNNHQGNDYQSTGMYSAPPSAHYGCLEPKQQQQQQQPNLMTTSTASTAILT 1120
                    |::.|||.|:...|  |:..:..||.             ||:|.:
Mouse  1116 --------QANLSNNMNNGAGD--SSEKHWKPPG-------------QQKPEV------------ 1145

  Fly  1121 NSPAKVKKINITENKMDQLEGKSERTN-------PFNQQQQLLLASNALKQGLGAYANTTLAAQM 1178
               |.::...:.:||::    |..|.|       |:||         :..|..|...|::.....
Mouse  1146 ---APIQYNIMEQNKLN----KDYRANDTIPPTIPYNQ---------SYDQNTGGSYNSSDRGSS 1194

  Fly  1179 ASGGGAGTLRRQRQPKTLYKSENNILGKSGLRQNTMNASVGAPSNGQMDFLTGGPP 1234
            .| |..|..:..|.||                         ||..|.|::....||
Mouse  1195 TS-GSQGHKKGARTPK-------------------------APKQGGMNWADLLPP 1224

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	NP_608592.2	Ig	22..117	CDD:299845	57/98 (58%)
		I-set	23..116	CDD:254352	56/96 (58%)
		I-set	122..211	CDD:254352	41/89 (46%)
		Ig	125..211	CDD:299845	39/86 (45%)
		I-set	215..302	CDD:254352	30/86 (35%)
		Ig	232..302	CDD:299845	26/69 (38%)
		I-set	306..401	CDD:254352	34/96 (35%)
		Ig	320..411	CDD:299845	33/93 (35%)
		I-set	412..492	CDD:254352	33/80 (41%)
		IGc2	429..489	CDD:197706	24/60 (40%)
		FN3	519..612	CDD:238020	29/92 (32%)
		FN3	644..735	CDD:238020	22/93 (24%)
		fn3	743..832	CDD:278470	28/90 (31%)
Robo1	XP_017172382.1	Ig1_Robo	100..199	CDD:143317	57/98 (58%)
		Ig2_Robo	207..291	CDD:143201	39/86 (45%)
		Ig3_Robo	312..380	CDD:143202	26/69 (38%)
		Ig4_Robo	401..490	CDD:143203	33/93 (35%)
		I-set	491..578	CDD:369462	36/87 (41%)
		FN3	597..683	CDD:238020	25/86 (29%)
		FN3	720..806	CDD:238020	20/85 (24%)
		fn3	814..897	CDD:365830	27/87 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6680
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2206
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm8796
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	10	9.670