DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and Robo3

DIOPT Version :9

Sequence 1:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster
Sequence 2:	XP_006510170.1	Gene:	Robo3 / 19649	MGIID:	1343102	Length:	1403	Species:	Mus musculus

Alignment Length:	1452	Identity:	407/1452 - (28%)
Similarity:	618/1452 - (42%)	Gaps:	276/1452 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    23 PRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPLKIL---PGSHRITLPAGGLFFLKV 84
            |||||.|.|..|.|.|||||.|:|||.|.|.|:|||:|..:...   |.:||:.||:|.|||.::
Mouse    64 PRIVEQPPDLVVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRI 128

  Fly    85 VNSRR-ETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECAAPRGI 148
            |:.|| ..|.|:|.|.|:|.||.|.||||:|:||||||:||..|.|..:|.|:.|::||..|:|.
Mouse   129 VHGRRSRPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGH 193

  Fly   149 PEPTVTWKKGGQKL-DLEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLATLKVH 212
            |||.||||||..|| :.||...:|   ||.|.:....::|.|.|.|:|.|..|.|||..|.|.|.
Mouse   194 PEPLVTWKKGKIKLKEEEGRITIR---GGKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVL 255

  Fly   213 VKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLEDRSLRLERVTI 277
            .:|..:|.|.:|.||..|.|.|.|.|.|||.|::.|.:  ..|.:|..|..:..|.||.:::|:.
Mouse   256 ERPSFLRRPINQVVLADAPVNFLCEVQGDPQPNLHWRK--DDGELPAGRYEIRSDHSLWIDQVSS 318

  Fly   278 ADEGEYSCEADNVVGAITAMGTLTVYAPPKFIQRPASKSVELGADTSFECRAIGNPKPTIFWTIK 342
            .|||.|:|.|:|.||...|.|:|:|:.||:|:.:|.:::|..||:.||:|...|||.|.|||..:
Mouse   319 EDEGTYTCVAENSVGRAEASGSLSVHVPPQFVTKPQNQTVAPGANVSFQCETKGNPPPAIFWQKE 383

  Fly   343 NNSTLIFP--GAPPLDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITSRVQLSLDS 405
            .:..|:||  ...|:.|.  |.:..|.  |.:|..:..|....: |.|::...||.::..|.:..
Mouse   384 GSQVLLFPSQSLQPMGRL--LVSPRGQ--LNITEVKIGDGGYYV-CQAVSVAGSILAKALLEIKG 443

  Fly   406 QE-DRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQ-PSSKLNITTSGDLII 468
            .. |..||||:.||.||||.:.|...|.|:.||.|.|.|.|.:|...:| ..|:.|:..:|.|.|
Mouse   444 ASIDGLPPIILQGPANQTLVLGSSVWLPCRVIGNPQPNIQWKKDERWLQGDDSQFNLMDNGTLHI 508

  Fly   469 SDLDRQQDQGLYTCVASSRAGKSTWSGFLRIEL-----------PTNPNIKFYRAPEQTKCPSAP 522
            :.: ::.|.|.|:|||.|..|::||:.:||.:.           |:||             |..|
Mouse   509 ASI-QEMDMGFYSCVAKSSIGEATWNSWLRKQEDWGASPGPATGPSNP-------------PGPP 559

  Fly   523 GQPKILNATASALTIVWPTSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVESLTQGA 587
            .||.:...||:::|:.|..:.::||:: ..|.:|.:......||..:|..:....:|:..|....
Mouse   560 SQPIVTEVTANSITLTWKPNPQSGATA-TSYVIEAFSQAAGNTWRTVADGVQLETYTISGLQPNT 623

  Fly   588 AYMFIVRAENSLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVVELLEAN 652
            .|:|:|||..:.|.|.|||:|||:.......:...:|.....|..|::|        .|.:.|..
Mouse   624 IYLFLVRAVGAWGLSEPSPVSEPVQTQADSSLSRPAEDPWKGQRGLAEV--------AVRMQEPT 680

  Fly   653 ASDSTTARLSWDIDSG-QYIEGFYLYAR--ELHSSEYKMVTLLNKGQGLSSCTVPGLAKASTYEF 714
            .....|.::||.:|.. |.::||.:..|  .|....:.|:.|.:..:  .|..:.||...:..:.
Mouse   681 VLGPRTLQVSWTVDGPVQLVQGFRVSWRIAGLDQGSWTMLDLQSPHK--QSTVLRGLPPGAQIQI 743

  Fly   715 FLVPFYKSIVGKPSNSRRMRTLEDVPEAPPYGME-AIQFNR-TSVFLKWLPPQPNRTRNGILTSY 777
            .:....:..:|..|........|:.|..||.|:. |:..:| :||.:.|.||.|:: |||::|.|
Mouse   744 KVQVQGQEGLGAESPFVTRSIPEEAPSGPPQGVAVALGGDRNSSVTVSWEPPLPSQ-RNGVITEY 807

  Fly   778 NVVVKGLDVHNTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAVLRIDAR 842
            .:...|    |.:|...|.:....|.::..:.|..|..|...|||||..|||..|.|.::::.
Mouse   808 QIWCLG----NESRFHLNRSAAGWARSVTFSGLLPGQIYRALVAAATSAGVGVASAPVLVQLP-- 866

  Fly   843 TQSLDTGYTRYP--------ISRDIAD---DFLTQTWFIVLLGSIIAIIVFLLG-ALVLFKRYQF 895
                      :|        :|..:|:   ..|.:..|  |.||..|....||| ...|::|.:
Mouse   867 ----------FPPAAEPGPEVSEGLAERLAKVLRKPAF--LAGSSAACGALLLGFCAALYRRQK- 918

  Fly   896 IKQTSLGSLHGNHAIGTVRKFPTLPLNGGGAVGAGGSNSTAAAGQSGHGLWIDPTSGVWRQAAGG 960
             ::..|.....:.|......||       .:.|..||:|....|       :.|.:..|...:..
Mouse   919 -QRKELSHYTASFAYTPAVSFP-------HSEGLSGSSSRPPMG-------LGPAAYPWLADSWP 968

  Fly   961 GNPGGV------GSCTTKEQLPG-----------YAQATAQQGQQPTLLPDYERLSPLNMPDYAE 1008
            ..|...      |||......|.           |...||:........|.|..:.|:.
Mouse   969 HPPRSPSAQEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGANASGEGPVYSTIDPVG------ 1027

  Fly  1009 VACSTFKSPTHGAPPMGGGGGGQSASLYDSCGAYATTNVVANVKLYQNRYATKPNQSSGSNNNNH 1073
            ....||    ||..|....|...:.|.|                       ..|..|.|  ::..
Mouse  1028 EELQTF----HGGFPQHSSGDPSTWSQY-----------------------APPEWSEG--DSGA 1063

  Fly  1074 QGNDYQSTG-------------MYSAPPSAHYGCLEPKQQQQQ-------------QQPNLMTTS 1112
            :|...:..|             :...|||....|.|..:::.:             ::.:|:.:|
Mouse  1064 RGGQGKLLGKPVQMPSLSWPEALPPPPPSCELSCPEGPEEELKGSSDLEEWCPPVPEKSHLVGSS 1128

  Fly  1113 TA----------------------STAILTNSPAKVKKINITENKMDQLEGKSER-----TNPFN 1150
            ::                      |||.||.||....:   ....:..|.....|     ::|.:
Mouse  1129 SSGACMVAPAPRDTPSPTSSYGQQSTATLTPSPPDPPQ---PPTDIPHLHQMPRRVPLGPSSPLS 1190

  Fly  1151 QQQQLLLASNALKQGLGA------YANTTLAAQMASGGGAGT---------------LRRQRQPK 1194
            ..|..|.:.:....||||      :|:.:.....||.....|               .|.:::||
Mouse  1191 VSQPALSSHDGRPVGLGAGPVLSYHASPSPVPSTASSAPGRTRQVTGEMTPPLHGHRARIRKKPK 1255

  Fly  1195 TL-YKSENNILGKSGLRQNTMNASVGAPSNGQMDFLTGGPPSEGGDFS-GLGLCNSTNQLLNDWA 1257
            .| |:.|:                    |.|.:......||.|...:. ||....|.:.|..:.:
Mouse  1256 ALPYRREH--------------------SPGDLPPPPLPPPEEETSWPLGLRAAGSMSSLERERS 1300

  Fly  1258 SSASI--AAP-GDYHFGSKQ---PSKQHLYVKAKDGTWSAVSSDAYQSFKHQQQHHPFLAGSGDN 1316
            ....:  |.| |....|:::   |....|...|:: .|......::.|.           |.|.:
Mouse  1301 GERRVVQAVPLGAQPLGAQRGPHPDAALLGCAAEE-AWLPYGRPSFLSH-----------GQGTS 1353

  Fly  1317 TKSLASVNSLAGDSKFLSSFGS 1338
            |.|.|..||..|.:....|.||
Mouse  1354 TCSTAGSNSSRGSNSSRGSRGS 1375

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	NP_608592.2	Ig	22..117	CDD:299845	51/97 (53%)
		I-set	23..116	CDD:254352	51/96 (53%)
		I-set	122..211	CDD:254352	40/89 (45%)
		Ig	125..211	CDD:299845	38/86 (44%)
		I-set	215..302	CDD:254352	35/86 (41%)
		Ig	232..302	CDD:299845	28/69 (41%)
		I-set	306..401	CDD:254352	30/96 (31%)
		Ig	320..411	CDD:299845	28/93 (30%)
		I-set	412..492	CDD:254352	33/80 (41%)
		IGc2	429..489	CDD:197706	22/60 (37%)
		FN3	519..612	CDD:238020	30/92 (33%)
		FN3	644..735	CDD:238020	18/93 (19%)
		fn3	743..832	CDD:278470	32/90 (36%)
Robo3	XP_006510170.1	Ig	64..162	CDD:386229	51/97 (53%)
		Ig	170..254	CDD:386229	38/86 (44%)
		Ig	275..343	CDD:386229	28/69 (41%)
		Ig4_Robo	361..450	CDD:143203	28/93 (30%)
		Ig	451..531	CDD:386229	33/80 (41%)
		FN3	564..649	CDD:238020	26/85 (31%)
		FN3	678..761	CDD:238020	17/84 (20%)
		FN3	771..864	CDD:238020	34/97 (35%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6680
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm8796
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	9	8.670