DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and sax-3

DIOPT Version :9

Sequence 1:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster
Sequence 2:	NP_001024990.1	Gene:	sax-3 / 180637	WormBaseID:	WBGene00004729	Length:	1273	Species:	Caenorhabditis elegans

Alignment Length:	1378	Identity:	394/1378 - (28%)
Similarity:	601/1378 - (43%)	Gaps:	280/1378 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    23 PRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPL---KILPGSHRITLPAGGLFFLKV 84
            |.|:|||||..|.|..||||||.|:.| |..|.|||||.|:   |....||||.|..|.||.|||
Worm    31 PVIIEHPIDVVVSRGSPATLNCGAKPS-TAKITWYKDGQPVITNKEQVNSHRIVLDTGSLFLLKV 94

  Fly    85 VNSR--RETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECAAPRG 147
            .:.:  :::|||.|:|.|.||.|..:|...:|::|:||::||:.|:..:...|:.|:|||:.|||
Worm    95 NSGKNGKDSDAGAYYCVASNEHGEVKSNEGSLKLAMLREDFRVRPRTVQALGGEMAVLECSPPRG 159

  Fly   148 IPEPTVTWKKGGQKLDLEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLATLKVH 212
            .|||.|:|:|..::|.::...|..:...|||.|....::|.|.|||:|.|.||.|.|:.|.|.|.
Worm   160 FPEPVVSWRKDDKELRIQDMPRYTLHSDGNLIIDPVDRSDSGTYQCVANNMVGERVSNPARLSVF 224

  Fly   213 VKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLED-RSLRLERVT 276
            .||...:.|.|.||..||:|.|.|||.|||.|.:.|.|  ....||:.|..:.:| |.||:|||.
Worm   225 EKPKFEQEPKDMTVDVGAAVLFDCRVTGDPQPQITWKR--KNEPMPVTRAYIAKDNRGLRIERVQ 287

  Fly   277 IADEGEYSCEADNVVGAITAMGTLTVYAPPKFIQRPASKSVELGADTSFECRAIGNPKPTIFWTI 341
            .:|||||.|.|.|..|.:.|...|.|.|||.|..:||.:||..|...:|||..:|.|.|..||:.
Worm   288 PSDEGEYVCYARNPAGTLEASAHLRVQAPPSFQTKPADQSVPAGGTATFECTLVGQPSPAYFWSK 352

  Fly   342 KNNSTLIFPGAPPLDRFHSLNTEEGHS------ILTLTRFQRTDKDLVILCNAMNEVASITSRVQ 400
            :....|:||         |..:.:|.:      .||:...::.|:...: |..||...|..|:..
Worm   353 EGQQDLLFP---------SYVSADGRTKVSPTGTLTIEEVRQVDEGAYV-CAGMNSAGSSLSKAA 407

  Fly   401 LSLDSQ------EDRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQ-PSSKL 458
            |.:.::      ..:|||.|..|..||||.:.|.|.|.|:|.|.|:|.|||.|||:|:. ..|::
Worm   408 LKVTTKAVTGNTPAKPPPTIEHGHQNQTLMVGSSAILPCQASGKPTPGISWLRDGLPIDITDSRI 472

  Fly   459 NITTSGDLIISDLDRQQDQGLYTCVASSRAGKSTWSGFLRIELPTNPNIKFYRAPEQTKCPSAPG 523
            :..::|.|.|:|| ::.|.|:|||:|.:..|:||||..|.:|..|: |.:|.|.|:.:..||:|.
Worm   473 SQHSTGSLHIADL-KKPDTGVYTCIAKNEDGESTWSASLTVEDHTS-NAQFVRMPDPSNFPSSPT 535

  Fly   524 QPKILNATASALTIVWPTSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVESLTQGAA 588
            ||.|:|.|.:.:.:.|.....:||....||.::.|..:..:||..|...::...:.::.|....:
Worm   536 QPIIVNVTDTEVELHWNAPSTSGAGPITGYIIQYYSPDLGQTWFNIPDYVASTEYRIKGLKPSHS 600

  Fly   589 YMFIVRAENSLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSD--------VETLLQANDV 645
            |||::||||..|...||..|..:|..|           ..:|:.|||        .|..|.:..:
Worm   601 YMFVIRAENEKGIGTPSVSSALVTTSK-----------PAAQVALSDKNKMDMAIAEKRLTSEQL 654

  Fly   646 VELLEANASDSTTARLSWDIDS-GQYIEGFYLYAR---ELHSSEYKMVTLLNKGQGLSSCTVPGL 706
            ::|.|....:||..||.|.... .:.|:|:|:..|   ..:.::|..||    .....:..|..|
Worm   655 IKLEEVKTINSTAVRLFWKKRKLEELIDGYYIKWRGPPRTNDNQYVNVT----SPSTENYVVSNL 715

  Fly   707 AKASTYEFFLVPFYK---SIVGKPSNSRRMRTLEDVPEAPPYGMEAIQFNRTSVFLKWLPPQPNR 768
            ...:.||||::|::.   ||.|.||||..:.|.|..|..||..:.....|.|::.:.|..|:.:.
Worm   716 MPFTNYEFFVIPYHSGVHSIHGAPSNSMDVLTAEAPPSLPPEDVRIRMLNLTTLRISWKAPKADG 780

  Fly   769 TRNGILTSYNVVVKGLDVHNTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSK 833
            . ||||..:.:|:.|...:|.    :|:|.:..|.::.|.:|.||:||.|.|||.:..|||
Worm   781 I-NGILKGFQIVIVGQAPNNN----RNITTNERAASVTLFHLVTGMTYKIRVAARSNGGVG---- 836

  Fly   834 PAVLRIDARTQSLDTGYTRYPISRDIADDFL-----TQTWFIVLLGS----IIAIIVFLLGALVL 889
                        :..|.:...:::|..:..|     .:::...|:..    :|.|:..|:..:|:
Worm   837 ------------VSHGTSEVIMNQDTLEKHLAAQQENESFLYGLINKSHVPVIVIVAILIIFVVI 889

  Fly   890 FKRYQFIKQTSLGSLHGNHAIGTVRKFPTLPLNGGGAVGAGGSNSTAAAGQSGHGLWIDPTSGVW 954
            ...|.:.:       :..::.|..|.|  :.:|.|            :...:.:.||
Worm   890 IIAYCYWR-------NSRNSDGKDRSF--IKINDG------------SVHMASNNLW-------- 925

  Fly   955 RQAAGGGNPGGVGSCTTKEQLPGYAQATAQQGQQPTLLPDYERLSPLNMPDYAEVACSTFKSPTH 1019
                                         ...|.|...|.|.                     |.
Worm   926 -----------------------------DVAQNPNQNPMYN---------------------TA 940

  Fly  1020 GAPPMGGGGGGQSASL-------YDSCGAYATTNVVANVKLYQNRYATKPNQSSGSNNNNHQGND 1077
            |...|....|....||       :::|..|:.|             ..:|    ||.::.|..
Worm   941 GRMTMNNRNGQALYSLTPNAQDFFNNCDDYSGT-------------MHRP----GSEHHYHYA-- 986

  Fly  1078 YQSTGMYSAPPSAHYGCLEPKQQQQQQQPNLMTTSTASTAILTN-SPAKVKKINITENKMDQLEG 1141
             |.||......|..||      .|....|:...|   :|.:|:| .||.:      .:||  |..
Worm   987 -QLTGGPGNAMSTFYG------NQYHDDPSPYAT---TTLVLSNQQPAWL------NDKM--LRA 1033

  Fly  1142 KSERTNPFNQQQQLLLASNALKQGLGAYANTTLAAQMAS---GGGAGTL------------RRQR 1191
            .:..|||...:..            ..||:.|...:..|   ..|.|||            |...
Worm  1034 PAMPTNPVPPEPP------------ARYADHTAGRRSRSSRASDGRGTLNGGLHHRTSGSQRSDS 1086

  Fly  1192 QPKT------LYKSENNILGK--SGLRQNTMNASVGAPSNGQMDFL---TGGPPSEGGDFSGLGL 1245
            .|.|      |:.|:.....|  :|.|:...|.::       |||:   ...||..||.......
Worm  1087 PPHTDVSYVQLHSSDGTGSSKERTGERRTPPNKTL-------MDFIPPPPSNPPPPGGHVYDDIF 1144

  Fly  1246 CNSTNQLLNDWASSASIAAPGDYHFGSKQPSKQHLYVKAKDGTWSAVSSDAYQSFKHQQQHHPFL 1310
            ..:|.:.||..::                 .::..|....||.::.|..:|..:.:::......|
Worm  1145 QTATRRQLNRGST-----------------PREDTYDSVSDGAFARVDVNARPTSRNRNLGGRPL 1192

  Fly  1311 AGSGDNTKSLASV 1323
            .|..|:....:|:
Worm  1193 KGKRDDDSQRSSL 1205

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	NP_608592.2	Ig	22..117	CDD:299845	48/98 (49%)
		I-set	23..116	CDD:254352	48/97 (49%)
		I-set	122..211	CDD:254352	36/88 (41%)
		Ig	125..211	CDD:299845	34/85 (40%)
		I-set	215..302	CDD:254352	39/87 (45%)
		Ig	232..302	CDD:299845	32/70 (46%)
		I-set	306..401	CDD:254352	28/100 (28%)
		Ig	320..411	CDD:299845	23/102 (23%)
		I-set	412..492	CDD:254352	35/80 (44%)
		IGc2	429..489	CDD:197706	26/60 (43%)
		FN3	519..612	CDD:238020	29/92 (32%)
		FN3	644..735	CDD:238020	28/97 (29%)
		fn3	743..832	CDD:278470	29/88 (33%)
sax-3	NP_001024990.1	Ig	30..129	CDD:299845	48/98 (49%)
		I-set	31..121	CDD:254352	46/90 (51%)
		I-set	135..223	CDD:254352	36/87 (41%)
		Ig2_Robo	137..223	CDD:143201	34/85 (40%)
		I-set	227..313	CDD:254352	39/87 (45%)
		Ig	244..313	CDD:299845	32/70 (46%)
		I-set	317..410	CDD:254352	29/102 (28%)
		Ig	331..411	CDD:299845	23/89 (26%)
		Ig	424..512	CDD:299845	41/88 (47%)
		I-set	432..512	CDD:254352	37/80 (46%)
		FN3	531..624	CDD:238020	29/92 (32%)
		FN3	659..747	CDD:238020	27/91 (30%)
		FN3	755..836	CDD:214495	27/85 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160167410
Domainoid	1	1.000	79	1.000	Domainoid score	I5610
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2206
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0.917438	Normalized mean entropy	S5483
OMA	1	1.010	-	-		QHG28892
OrthoDB	1	1.010	-	-		D173520at2759
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		otm14705
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	12	11.710