DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and robo1

DIOPT Version :9

Sequence 1:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster
Sequence 2:	XP_012811710.3	Gene:	robo1 / 100124716	XenbaseID:	XB-GENE-948809	Length:	1680	Species:	Xenopus tropicalis

Alignment Length:	1242	Identity:	391/1242 - (31%)
Similarity:	591/1242 - (47%)	Gaps:	192/1242 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 QGSHPPRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPL---KILPGSHRITLPAGGL 79
            |...||||||||.|..|.:.|||||||||||.|||||:|||.|..:   |..|.|||:.||:|.|
Frog    81 QEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSL 145

  Fly    80 FFLKVVNSRR-ETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECA 143
            |||::|:.|: ..|.|:|.|.|:|.||.|.|.||:|:||:|||:||..|.:..:|.|:.|::||.
Frog   146 FFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQ 210

  Fly   144 APRGIPEPTVTWKKGGQKLDLEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLAT 208
            .|||.||||::|||.|..:|.:..:|:.| .||.|.|...|::|.|:|.|:..|.||.|||.:|.
Frog   211 PPRGHPEPTISWKKDGTPIDDDKDERITI-RGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAE 274

  Fly   209 LKVHVKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLEDRSLRLE 273
            |.|..:|..:|.|.:..|....||.|.|...|||:|.|.|.:  ..|::|..|..:.:|.:|::.
Frog   275 LTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRK--DDGDLPKSRYEIRDDHTLKIR 337

  Fly   274 RVTIADEGEYSCEADNVVGAITAMGTLTVYA---PPKFIQRPASKSVELGADTSFECRAIGNPKP 335
            :||..|.|.|:|.|:|:||...|...|||..   ||.|:.:|..:.|.||...:|:|.|.|||:|
Frog   338 KVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQP 402

  Fly   336 TIFWTIKNNSTLIFPGAPP--LDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITSR 398
            .|||..:.:..|:|...||  ..||....|.:    ||:|..|::|....| |..:|...|||::
Frog   403 AIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGD----LTITTVQKSDVGYYI-CQTLNVAGSITTK 462

  Fly   399 VQLSL-DSQEDRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDG--IPVQPSSKLNI 460
            ..|.: |...|||||:|..||||||:.:.....:.|.|.|:|.|||.|.:||  ||.| .|::..
Frog   463 AFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQ-DSRIKQ 526

  Fly   461 TTSGDLIISDLDRQQDQGLYTCVASSRAGKSTWSGFLRIE---LPTNPNIKFYRAPEQTKCPSAP 522
            ..||.|.|. ..:..|.|.|||.||:.:|::.||.::.::   :|..|.    ||.:....||||
Frog   527 QDSGALQIR-YAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPP----RATDPNLIPSAP 586

  Fly   523 GQPKILNATASALTIVWPTSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVESLTQGA 587
            .:|::.:.:.:.:|:.|..:..:||:. ..|.:|.:......:|..:|..:....|.|::|...|
Frog   587 SKPEVTDVSKNTVTLSWQPNLNSGATP-TSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNA 650

  Fly   588 AYMFIVRAENSLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVVELLEAN 652
            .|:|:|||.|:.|.|.||.||:|:....:.....|.:.... |..|.||        |:.|....
Frog   651 IYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQV-QRELGDV--------VLHLHNPT 706

  Fly   653 ASDSTTARLSWDID-SGQYIEGFYLYARELHSSEYKMVTLLNKGQGL-----------SSCTVPG 705
            ...|::..:.|.:| ..|||:|:.:..|.  |:|       |:|:..           :|..:|.
Frog   707 ILSSSSIEVHWTVDQQSQYIQGYKILYRP--SAE-------NRGESAWSIFEVRTPAKNSAVIPE 762

  Fly   706 LAKASTYEFFLVPFYKSIVGKPSNSRRMRTLEDVPEAPPYGMEAIQ--FNRTSVFLKWLPPQPNR 768
            |.|...||....||:....|..|..|..:|:|:.|.|||..:...:  .|.|::.:.|.|| |..
Frog   763 LRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPP-PED 826

  Fly   769 TRNGILTSYNVVVKGLDVHNTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSK 833
            |:||::..|.|...|    |.:|...|.|:|.:..::::.:|..|:.|.:.|||:|..|.|..|:
Frog   827 TQNGMVQEYKVWCLG----NESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSE 887

  Fly   834 PAVLRIDARTQSLDTGYTRYPISRDIADDFLTQTWFIVLLGSIIAIIVFLLGALVLFKRYQFIKQ 898
            |..:::|:....:..| .:..:::.|: |.:.|..||..:|:...||      |::|..:.:..:
Frog   888 PNYIQLDSFGNPVPPG-DQVSLAQQIS-DVVKQPAFIAGIGAACWII------LMVFSIWLYRHR 944

  Fly   899 TSLGSLHGNHAIGTVRKFPTLPL-------NGGGAVGAGGSNSTAAAGQSGHGLWIDPTSGVWRQ 956
            .....|..::|  .:||.|:...       .||.||.:.|........::.:..|:..|   |..
Frog   945 KKRNGLTSSYA--GIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISETANQPWLADT---WPN 1004

  Fly   957 AAGGGNPGGVGSCTTKEQLPGYAQATAQQGQQPT-LLPDY-----ERLSPLNMPD---YAEVACS 1012
            .....|...:..||:..   |.:.:......:|. .:.:|     .:.:.|.:||   |.:|..|
Frog  1005 TGNNHNECPINCCTSGN---GNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLS 1066

  Fly  1013 -------TFKSPT---------HGAPPMGGGGGGQSASLYDSCGAYATTNVVANVKLYQNRYATK 1061
                   ||.||.         .|.|.                 .||||.::
Frog  1067 NKINEMKTFNSPNLKDGRFVNPAGQPT-----------------PYATTQLI------------- 1101

  Fly  1062 PNQSSGSNNNNHQGNDYQSTGMYSAPPSAHYGCLEPKQQQQQQQPNLMTTSTASTAILTNSPAKV 1126
              ||:.|||.|....|...  .:..||     |        ||:|::             :|
Frog  1102 --QSNISNNINTSAGDVDE--KHWKPP-----C--------QQKPDV-------------TP--- 1133

  Fly  1127 KKINITE-NKMDQLEGKSE---RTNPFNQQQQLLLASNALKQGLGAYANTTLAAQMASGGGAGTL 1187
            .:.||.| ||:::....|:   .|.|:||...         |..|...|::......| |..|..
Frog  1134 MQYNIMEQNKINKDYRASDTIPSTIPYNQSHD---------QNAGGSYNSSDRGSSTS-GSQGHK 1188

  Fly  1188 RRQRQPK 1194
            :..|.||
Frog  1189 KSTRTPK 1195

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	NP_608592.2	Ig	22..117	CDD:299845	57/98 (58%)
		I-set	23..116	CDD:254352	56/96 (58%)
		I-set	122..211	CDD:254352	39/88 (44%)
		Ig	125..211	CDD:299845	37/85 (44%)
		I-set	215..302	CDD:254352	31/86 (36%)
		Ig	232..302	CDD:299845	26/69 (38%)
		I-set	306..401	CDD:254352	34/96 (35%)
		Ig	320..411	CDD:299845	33/93 (35%)
		I-set	412..492	CDD:254352	34/81 (42%)
		IGc2	429..489	CDD:197706	25/61 (41%)
		FN3	519..612	CDD:238020	30/92 (33%)
		FN3	644..735	CDD:238020	25/102 (25%)
		fn3	743..832	CDD:278470	28/90 (31%)
robo1	XP_012811710.3	IgC_1_Robo	86..184	CDD:409490	56/97 (58%)
		Ig strand B	103..107	CDD:409490	3/3 (100%)
		Ig strand C	116..120	CDD:409490	2/3 (67%)
		Ig strand E	143..147	CDD:409490	2/3 (67%)
		Ig strand F	162..167	CDD:409490	2/4 (50%)
		Ig strand G	176..179	CDD:409490	1/2 (50%)
		IgI_2_Robo	191..277	CDD:409389	38/86 (44%)
		Ig strand B	205..209	CDD:409389	1/3 (33%)
		Ig strand C	219..223	CDD:409389	1/3 (33%)
		Ig strand E	242..246	CDD:409389	2/3 (67%)
		Ig strand F	256..261	CDD:409389	2/4 (50%)
		Ig strand G	270..273	CDD:409389	1/2 (50%)
		IgI_3_Robo	284..366	CDD:409390	30/83 (36%)
		Ig strand B	298..302	CDD:409390	2/3 (67%)
		Ig strand C	311..315	CDD:409390	1/3 (33%)
		Ig strand E	332..336	CDD:409390	1/3 (33%)
		Ig strand F	346..351	CDD:409390	2/4 (50%)
		Ig strand G	359..362	CDD:409390	1/2 (50%)
		IgI_4_Robo	374..471	CDD:409391	35/101 (35%)
		Ig strand B	390..394	CDD:409391	1/3 (33%)
		Ig strand C	403..407	CDD:409391	2/3 (67%)
		Ig strand E	433..437	CDD:409391	1/7 (14%)
		Ig strand F	447..452	CDD:409391	2/5 (40%)
		Ig strand G	460..463	CDD:409391	1/2 (50%)
		IgI_5_Robo	478..564	CDD:409544	35/87 (40%)
		Ig strand B	494..498	CDD:409544	0/3 (0%)
		Ig strand C	507..511	CDD:409544	2/3 (67%)
		Ig strand E	530..534	CDD:409544	2/3 (67%)
		Ig strand F	544..549	CDD:409544	3/4 (75%)
		Ig strand G	557..560	CDD:409544	1/2 (50%)
		FN3	583..669	CDD:238020	26/86 (30%)
		FN3	706..792	CDD:238020	23/94 (24%)
		fn3	800..886	CDD:394996	28/90 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	103	1.000	Domainoid score	I6691
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H2206
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm9461
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	6	6.000