DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mtRNApol and RPO41

DIOPT Version :9

Sequence 1:	NP_001259853.1	Gene:	mtRNApol / 33285	FlyBaseID:	FBgn0261938	Length:	1369	Species:	Drosophila melanogaster
Sequence 2:	NP_116617.1	Gene:	RPO41 / 850507	SGDID:	S000001858	Length:	1351	Species:	Saccharomyces cerevisiae

Alignment Length:	1445	Identity:	396/1445 - (27%)
Similarity:	606/1445 - (41%)	Gaps:	322/1445 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly   140 ILERRAREDRKDKKTKCVLDLAQRQPVEYQKIDMEALDGSILESGVYDDRPMLFFGKD-EYTKQV 203
            :|:||....:..|..|...|......:....:...:...|...||:........|.|: :..|..
Yeast    14 LLQRRLISSKGSKLFKPSPDSTSTILISEDPLVTGSSPTSSTTSGIISSNDFPLFNKNRKDAKSS 78

  Fly   204 IA----NPEEVQ---------NILKSFQNFRDIIE-------SMENDQHEADFGLSTTKVHPEEH 248
            |:    ||.|::         :.:.|....||:::       .::::..:..|.:..:.....||
Yeast    79 ISYQWKNPSELEFDPFNKSHASAVTSMTRTRDVMQLWSLLEACLQSNLMKRAFSILESLYLVPEH 143

  Fly   249 EMLDPFEAIEPYISPLDK-SKRSVTTVAVPNASAVTTKSKKRFKSKVHVNEEQERLAKQRALHIN 312
            :.    ..||.|...|:. ||.......:.....:|...:..||. |:.|::...:....||:.:
Yeast   144 KQ----RFIEDYNMYLNSFSKNDPNFPILKMNEKLTNDLETSFKD-VNYNDKTLAIMIHHALNFH 203

  Fly   313 LNTYLNVCVSANMLNRALSTIIAYRGKVRKSSLPHMSE-----GLITIDLYNILLHGYAGKGSFD 372
            ..|      |:.:|...:|..:       |.|:..:.|     .::||...|||::.
Yeast   204 STT------SSMLLKPIISAYL-------KMSVNGIREIFSCLDILTISDLNILMND-------- 247

  Fly   373 RAQEVFKLIEEDGLSFNEQTYAAIFECLGRLEPDEENHQYISEYVSKAEKEGFS----------- 426
                 .|:|....|   ..:...|.|.| .|.|...|:....|.::|.|.|..|
Yeast   248 -----LKVITPSQL---PNSVRPILESL-TLSPTPVNNIENEEGLNKVEAENDSKLHKASNASSD 303

  Fly   427 ---------LNQIMDRSKFVADQRD----IALDAI--RRLRPDFKPVYVPPQLGYDNELLDHL-- 474
                     |.::..........:|    ||:|.|  |.:|.....:.:.|:   ..|.:...
Yeast   304 SIKKPSLDPLREVSFHGSTEVLSKDAEKLIAVDTIGMRVIRHTLLGLSLTPE---QKEQISKFKF 365

  Fly   475 --NEHVIPVGAKKEPGKAD--------YAIMNS------KRGYTAA--------------QLEQL 509
              |::|:    |.:|.|.|        :.|.||      |:.:.:|              :..:.
Yeast   366 DANDNVL----KMKPTKNDDNNNSINFFEIYNSLPTLEEKKAFESALNIFNQDRQKVLENRATEA 426

  Fly   510 AREQLKIELD-----GSITIKSIEKS----------------KEFANSEKCRELLKE-LEDTWRK 552
            |||:.|.:.:     |.|   ||||:                ||..|  .||.||.| |.|  :|
Yeast   427 ARERWKHDFEEAKARGDI---SIEKNLNVKLWKWYNEMLPLVKEEIN--HCRSLLSEKLSD--KK 484

  Fly   553 QISAAIVRDLNTLRAQVRFKPHGFMNYYTYLKTLDASHFADILIKELYKL------AEGSETFSP 611
            .::..   |.|.|            .|..||..:|......|.|.||.||      .||..|...
Yeast   485 GLNKV---DTNRL------------GYGPYLTLIDPGKMCVITILELLKLNSTGGVIEGMRTARA 534

  Fly   612 TVGQLYKELGQKVQQRYQIEQ--KKHNGTLEKIGEIYSSYCELWGSGMSQDNTRQAWQRLVYEQR 674
            .:     .:|:.::..::.||  |..:.....:.:....:.:|      ..|.:..::....||.
Yeast   535 VI-----SVGKAIEMEFRSEQVLKSESQAFRDVNKKSPEFKKL------VQNAKSVFRSSQIEQS 588

  Fly   675 ESGPSMDLLEVPWPSNVLTGVGRFLYNILMRDIKIDAHIMRQKSKSKAAAQPQNLLPAFYTLFRN 739
                     ::.||.::...:|..|.::|::..|:....:...:|:|...:    .|||...::.
Yeast   589 ---------KILWPQSIRARIGSVLISMLIQVAKVSVQGVDPVTKAKVHGE----APAFAHGYQY 640

  Fly   740 QGRFVKEEVKPHPVLSRLFRASRQQTLTFDSNLVPMLCPPQPWSTPHNGGYLLNKTELIRLPHQA 804
            ........:|.|..|.|.....| ...:....|:|||..|:||....:|||...::.|:|.....
Yeast   641 HNGSKLGVLKIHKTLIRQLNGER-LIASVQPQLLPMLVEPKPWVNWRSGGYHYTQSTLLRTKDSP 704

  Fly   805 IQQWDRIMASNPQHLYPALDSLNQLASVPWRVNTQLLDVIIKVFQNGGDVKLDVP--QPPSSLPP 867
            .|......||:...:....|.||.|...||.||.::.||:.:|: |.|:..||:|  |....|||
Yeast   705 EQVAYLKAASDNGDIDRVYDGLNVLGRTPWTVNRKVFDVVSQVW-NKGEGFLDIPGAQDEMVLPP 768

  Fly   868 LPTLPAKDAEGNATSSEDRAKQFRDKLGHRRKQAEMYSLWCDALYRLSLAQHYRDKVFWLPHNMD 932
            .|  |.........:.:.:.|...:|....|...      ||..|:|.:|:.:..:..:.|||:|
Yeast   769 AP--PKNSDPSILRAWKLQVKTIANKFSSDRSNR------CDTNYKLEIARAFLGEKLYFPHNLD 825

  Fly   933 FRGRVYPVPPHLNHLGSDLARSMLIFDQAQPLGVDGLSWLKLHCINLTGLKKRDSVRERLLYAEE 997
            ||||.||:.||.||||:|::|.:|||...:.||..||.|||:|..||.|..|. .:::|:.:.|.
Yeast   826 FRGRAYPLSPHFNHLGNDMSRGLLIFWHGKKLGPSGLKWLKIHLSNLFGFDKL-PLKDRVAFTES 889

  Fly   998 IMPDILDSADNPLTGRMWWSKSDEPWQTLACCMEIANVHRCPDPAAYLSRFPIHQDGSCNGLQHY 1062
            .:.||.|||:|||||..||:.:|:|||.||.|.|:..|.:..:|..::|..|:||||:|||||||
Yeast   890 HLQDIKDSAENPLTGDRWWTTADKPWQALATCFELNEVMKMDNPEEFISHQPVHQDGTCNGLQHY 954

  Fly  1063 AALGRDEAGARSVNLAPSAIPQDVYSAVAALVEKSRKADAQNGLHVAEALAGFVRRKVIKQTVMT 1127
            ||||.|..||..|||.||..|||||:.||.||:|..:..|:.|...|:.|...:.|||:||||||
Yeast   955 AALGGDVEGATQVNLVPSDKPQDVYAHVARLVQKRLEIAAEKGDENAKILKDKITRKVVKQTVMT 1019

  Fly  1128 TVYGVTRYGARLQIARQLKDIDDFPKDWVWPASTYLTTKTFESLREMFTSTREIQDWFTECARLI 1192
            .|||||..||..|||:||..|.|..|:.: ..|.|||...|.::||:|.|...||||..|.|:.|
Yeast  1020 NVYGVTYVGATFQIAKQLSPIFDDRKESL-DFSKYLTKHVFSAIRELFHSAHLIQDWLGESAKRI 1083

  Fly  1193 SGVCSQNVE-----------------WVTPLGLPVVQPYNRQEMKHSPRSGFKVSANMPMDLYER 1240
            |.....:|:                 |.||||||:||||..:..|       :|..|:.......
Yeast  1084 SKSIRLDVDEKSFKNGNKPDFMSSVIWTTPLGLPIVQPYREESKK-------QVETNLQTVFISD 1141

  Fly  1241 P------NILKQKNAFPPNFIHSLDSSHMMLTSLHCERQGITFVSVHDCFWTHANTVPELNRMCR 1299
            |      |..:||...|||||||||:|||:|::..|.:||:.|.||||.:||||:.:..:|.:.|
Yeast  1142 PFAVNPVNARRQKAGLPPNFIHSLDASHMLLSAAECGKQGLDFASVHDSYWTHASDIDTMNVVLR 1206

  Fly  1300 EQFVALHSQPILEQLSE--------------FMRHT----------------------------Y 1322
            |||:.||...::.:|.|              ..|.|                            :
Yeast  1207 EQFIKLHEVDLVLRLKEEFDQRYKNYVKIGKLKRSTDLAQKIIRIRKDLSRKLGRSTTLADEIYF 1271

  Fly  1323 SFKDSDFINDGSVED--LSKRQLNRI-------------------------------LKQMPQKG 1354
            ..|..:.:|...:||  :.::.:..:                               |.::|.||
Yeast  1272 EKKRQELLNSPLIEDRNVGEKMVTTVSLFEDITDLDALELENGGDENSGMSVLLPLRLPEIPPKG 1336

  Fly  1355 DFDLRNVLDSVYFFS 1369
            |||:..:.:|.||||
Yeast  1337 DFDVTVLRNSQYFFS 1351

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mtRNApol	NP_001259853.1	RPO41	286..1369	CDD:227439	365/1275 (29%)
		PPR repeat	314..342	CDD:276811	4/27 (15%)
		PPR repeat	355..384	CDD:276811	6/28 (21%)
		PPR	358..386	CDD:279828	5/27 (19%)
		RPOL_N	513..829	CDD:291372	76/345 (22%)
		RNA_pol	962..1368	CDD:279308	184/503 (37%)
RPO41	NP_116617.1	RPO41	79..1351	CDD:227439	382/1378 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346093
Domainoid	1	1.000	316	1.000	Domainoid score	I192
eggNOG	1	0.900	-	-		E1_COG5108
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	438	1.000	Inparanoid score	I265
Isobase	1	0.950	-	0	Normalized mean entropy	S2671
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003398
OrthoInspector	1	1.000	-	-		oto100387
orthoMCL	1	0.900	-	-		OOG6_101824
Panther	1	1.100	-	-	LDO	PTHR10102
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1209
SonicParanoid	1	1.000	-	-		X2294
TreeFam	1	0.960	-	-
	14	13.730