DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mtRNApol and Polrmt

DIOPT Version :9

Sequence 1:	NP_001259853.1	Gene:	mtRNApol / 33285	FlyBaseID:	FBgn0261938	Length:	1369	Species:	Drosophila melanogaster
Sequence 2:	XP_006240921.1	Gene:	Polrmt / 299604	RGDID:	1304599	Length:	1204	Species:	Rattus norvegicus

Alignment Length:	1319	Identity:	508/1319 - (38%)
Similarity:	716/1319 - (54%)	Gaps:	185/1319 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    87 VAQAKKKRLRKPRYAK------YVELLEVTEQAISERRS------RVKRLKSKKLADFIHKAQQQ 139
            :..::::....||...      :.|||||.|..:.:.|:      |||:::       :.:..|.
  Rat    35 LGSSRRRSAASPREQAVQREWGHAELLEVLEARVRQLRAEGAPEMRVKKVQ-------VDQPPQG 92

  Fly   140 ILERRAREDRKDKKTK----CVLDLAQRQPVEYQKIDMEALDGSILESGVYDDRPMLFFGKDEYT 200
            ...|.|::...:||.|    ...|| |:||:|.:.:..|.
  Rat    93 RSSRWAQKLEAEKKVKQRHQAKADL-QKQPLESRTLHTEP------------------------- 131

  Fly   201 KQVIANPEEVQNILKSFQNFRDIIESMENDQHEADFGLSTTKVHPEEHEMLDPFEAIEPYISPLD 265
                                |...:.:....|::..|...:.   ||.::....:|:      |.
  Rat   132 --------------------RIRSKKLAGYLHQSKKGAPKSS---EEEQLARVLQAV------LA 167

  Fly   266 K-SKRSVTTVAVPNASAVTTKSKKRFKSKVHVNEEQERLAKQRALHINLNTYLNVCVSANMLNRA 329
            | ..|...|.|...|.||.|          |:...|::          |.|:...||....|..|
  Rat   168 KLGSREAGTPARTKAKAVET----------HILFLQQK----------LRTFFECCVCTGQLPLA 212

  Fly   330 LSTIIAYRGKVRKSSLPHMSEGLITIDLYNILLHGYAGKGSFDRAQEVFKLIEEDGLSFNEQTYA 394
            ...::.:.....|       :.::|:|:||::|.|:|.||||.....||.::.:.|||.:..:||
  Rat   213 HHMLVTHHNNQDK-------QQVLTLDMYNMVLLGWARKGSFRELVYVFIMLTDAGLSPDLHSYA 270

  Fly   395 AIFECLGRLEPDEENHQYISEYVSKAEKEGFSLNQIMDRSKFVADQRDIALDAIRRLRPDFKPVY 459
            |..:|:||.:.|...   |...:.:..:|||....:........:.|...|.|:.:..|.|.|
  Rat   271 AALQCMGRRDQDAHT---IQRCLKQMAEEGFQPQLLFTNLVLEEEDRAALLRAVVKGEPAFCP-- 330

  Fly   460 VPPQLGYDNELLDHLNEHVIPVGAKKEPGKADYAIMNSKRGYTAAQLEQLAREQLKIELDGSITI 524
             |||..         |....||..|....|.:..:...|.......|:.|..:||.:||..|:::
  Rat   331 -PPQAS---------NLVSTPVLLKDFYSKQEGPVSYPKLHLPLDTLQDLFYQQLHVELSSSVSV 385

  Fly   525 KSIEK----SKEFANSEKCRELLKELEDTWRKQISAAIVRDLNTLRAQVRFKPH-GFMNYYTYLK 584
            :|:|:    :||..   :.|:.||.|...|..:    ::|.|...:|::..:.: |....|.:|.
  Rat   386 QSVEEAPVMTKEVI---EARKTLKALRSKWEVE----LLRVLRETKAKMGSQAYEGQPTLYPFLC 443

  Fly   585 TLDASHFADILIKELYKL-AEGSETFSPTVGQLYKELGQKVQQRYQIEQKKHNGTLEKIGEIYSS 648
            .|....|..||::.|..| |:|...|     ||.:.||.:|..|:.::||:....::|:|:.||.
  Rat   444 LLSEGEFVSILMQALQVLPAQGEPLF-----QLAQNLGLQVFNRHLVKQKQVTNHVQKLGQQYSQ 503

  Fly   649 YCELWGSG--MSQDNTRQAWQRLVYEQRESGPSMDLLEVPWPSNVLTGVGRFLYNILMRDIKIDA 711
            |..|..|.  ::....|:.|..|       |......:.||...||..:|:.|..:|::.:
  Rat   504 YLRLLASDTQVAPCLPREYWDSL-------GSLETPAQQPWSVPVLLQLGKQLAELLVQAV---- 557

  Fly   712 HIMRQKSKSKAAAQ-PQNLLPAFYTLFRNQGRFVKEEVKPHPVLSRLFRASRQQTLTFDSNLVPM 775
                |..:|.||.. .||.:|..|.::..:.......:||||..:.|...:.:.||||::..|||
  Rat   558 ----QMPRSLAARHGAQNFIPVLYHVYSFRSYRQVGILKPHPAFTHLLETAAEPTLTFETTEVPM 618

  Fly   776 LCPPQPWSTPHNGGYLLNKTELIRLPHQAIQQWDRIMASNPQHLYPALDSLNQLASVPWRVNTQL 840
            ||||.||::.|:|.|||:.|:::|......|....:....|..|:.|||:|.||.:..||||.:|
  Rat   619 LCPPLPWTSLHSGAYLLSSTKIMRATEGTTQHQRLLERCPPAQLHGALDALTQLGNCAWRVNGRL 683

  Fly   841 LDVIIKVFQNGGDVKLDVPQPPSSLPPLPT---LPAKDAEGNATSSEDRAKQFRDKLGHRRKQA- 901
            ||:::::|::.|.::|.|| ||.|..|.|.   ||       ..|:.....:.|.:|....|.|
  Rat   684 LDLVLQIFRDKGCMRLGVP-PPRSEAPRPAKHQLP-------PGSTPVHKSELRKELARCLKAAR 740

  Fly   902 EMYSLWCDALYRLSLAQHYRDKVFWLPHNMDFRGRVYPVPPHLNHLGSDLARSMLIFDQAQPLGV 966
            ||:||..:||||||||||.||:|||||||||||||.||.|||.|||||||||::|.|.:.:|||.
  Rat   741 EMHSLRSEALYRLSLAQHLRDRVFWLPHNMDFRGRTYPCPPHFNHLGSDLARALLEFAEGRPLGP 805

  Fly   967 DGLSWLKLHCINLTGLKKRDSVRERLLYAEEIMPDILDSADNPLTGRMWWSKSDEPWQTLACCME 1031
            .||.|||:|.||||||:||||:|.||.:|:|:|.:|||||||||||:.||.|:||||||||||||
  Rat   806 RGLDWLKIHLINLTGLRKRDSLRMRLAFADEVMGEILDSADNPLTGQKWWMKADEPWQTLACCME 870

  Fly  1032 IANVHRCPDPAAYLSRFPIHQDGSCNGLQHYAALGRDEAGARSVNLAPSAIPQDVYSAVAALVEK 1096
            :|...|.|||.||:|..|:||||||||||||||||||..||.||||.||.:|||||..||..||:
  Rat   871 VAQAVRSPDPTAYVSHLPVHQDGSCNGLQHYAALGRDSVGAASVNLTPSDLPQDVYREVATQVEE 935

  Fly  1097 SRKADAQNGLHVAEALAGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIDDFPKDWVWPAST 1161
            .|:.||:.||.||:||.|||.|||:||||||.||||||||.||||.::|:::.|||:::||.||.
  Rat   936 LRQQDAKEGLQVAQALEGFVSRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASH 1000

  Fly  1162 YLTTKTFESLREMFTSTREIQDWFTECARLISGVCSQNVEWVTPLGLPVVQPYNRQEMKHSPRSG 1226
            ||..:.|:||:|||:|||.||.|.||.|.|||. ....||||||||:|::|||:| |.|...:.|
  Rat  1001 YLVRQVFKSLQEMFSSTRAIQRWLTESANLISH-AGWPVEWVTPLGIPIIQPYHR-ESKIQVKGG 1063

  Fly  1227 FK-VSANMPMDLYERPNILKQKNAFPPNFIHSLDSSHMMLTSLHCERQGITFVSVHDCFWTHANT 1290
            .: ::....:|..::||.|||||.|||||||||||||||||:|||.|:|:|||||||||||||..
  Rat  1064 LQSITFTSSVDDSQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCFWTHAAD 1128

  Fly  1291 VPELNRMCREQFVALHSQPILEQLSEFMRHTY-----SFKDSDFINDGSVEDLSKRQLNRILKQM 1350
            :|.:|.:||||||.|||:||||.|::|:...:     |.|.|:      :..::|  |...|:.:
  Rat  1129 IPMMNEVCREQFVRLHSEPILEDLAKFLEKRFCSVSKSLKPSE------LALITK--LQETLRSL 1185

  Fly  1351 PQKGDFDLRNVLDSVYFFS 1369
            |:.|.|||..|:.|.||||
  Rat  1186 PETGTFDLGQVIRSTYFFS 1204

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mtRNApol	NP_001259853.1	RPO41	286..1369	CDD:227439	466/1101 (42%)
		PPR repeat	314..342	CDD:276811	5/27 (19%)
		PPR repeat	355..384	CDD:276811	12/28 (43%)
		PPR	358..386	CDD:279828	11/27 (41%)
		RPOL_N	513..829	CDD:291372	95/324 (29%)
		RNA_pol	962..1368	CDD:279308	246/411 (60%)
Polrmt	XP_006240921.1	ftsN	<22..>164	CDD:274041	30/184 (16%)
		PPR repeat	198..225	CDD:276811	5/26 (19%)
		PPR repeat	231..260	CDD:276811	12/28 (43%)
		PPR repeat	266..296	CDD:276811	8/32 (25%)
		RPOL_N	391..673	CDD:291372	89/308 (29%)
		RNA_pol	801..1203	CDD:279308	246/411 (60%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166352838
Domainoid	1	1.000	506	1.000	Domainoid score	I317
eggNOG	1	0.900	-	-		E1_COG5108
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H37996
Inparanoid	1	1.050	804	1.000	Inparanoid score	I467
OMA	1	1.010	-	-		QHG47603
OrthoDB	1	1.010	-	-		D135329at2759
OrthoFinder	1	1.000	-	-		FOG0003398
OrthoInspector	1	1.000	-	-		oto98891
orthoMCL	1	0.900	-	-		OOG6_101824
Panther	1	1.100	-	-	LDO	PTHR10102
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X2294
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	16	15.770