DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mtRNApol and rpo41

DIOPT Version :9

Sequence 1:	NP_001259853.1	Gene:	mtRNApol / 33285	FlyBaseID:	FBgn0261938	Length:	1369	Species:	Drosophila melanogaster
Sequence 2:	NP_594459.2	Gene:	rpo41 / 2542687	PomBaseID:	SPAC26H5.12	Length:	1154	Species:	Schizosaccharomyces pombe

Alignment Length:	1257	Identity:	377/1257 - (29%)
Similarity:	577/1257 - (45%)	Gaps:	256/1257 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly   240 TTKVHPEEHEMLDPFEAIEPYISPLDKSKRSVTTVAVPNASAVTTKSKKRFKSKVHVNEEQERLA 304
            |.::|.:    ..|.||.|||                          |...|||:         .
pombe    27 TQRLHTD----YMPIEAYEPY--------------------------KNELKSKI---------G 52

  Fly   305 KQRALHINLN----TYLNVCVSANMLNRALSTIIAYRG------KVRKSSLPHMSEGLITIDLYN 359
            |...:.::..    :....||              |.|      ::.||.:.|.....|.:.:.|
pombe    53 KDFIIDLSYKSGTASLFEACV--------------YNGDFLRSKQLLKSFIDHNKGDKILLPMIN 103

  Fly   360 ILLHGYAGKGSF---DRAQEVFKLIEEDGLSFNEQTYAAIFECLGRLEPDEEN------HQYISE 415
            :.:.....:|||   |......:|:::..|:.:..|||.:  |...|.|.:..      |:.|..
pombe   104 LYIREIIQRGSFELTDVLSNAKELLQQARLNGDSLTYALL--CQASLNPTQRQLGLPVLHELIHN 166

  Fly   416 YVSKAEKEGFSLNQIMDRSKFVADQRDIALDAIRRLRPDFKPV--YVPPQLGYDNELLDHLNEHV 478
            :.|   ..|..::.:|..|.|..::..:.:|.:.      .|:  :.|.||    :||...|..:
pombe   167 WRS---ANGKVIDILMHESVFSPEEVKLIMDQLN------IPINNFTPSQL----QLLGITNSTI 218

  Fly   479 IPVGAKKEPGK---ADYAIMNSKRGY-----TAAQLEQLAREQLKIELDGSITIKSIEKSKEFAN 535
            :   .:.|.||   .|.::...:...     .:|.|..| |..|...|..||.:...|.|.||.|
pombe   219 V---GESENGKDQNGDSSLKEKQPDVETTVTKSANLNAL-RSSLSSLLTESIDLPIDEVSLEFGN 279

  Fly   536 S------------EK-----CRELLK---------------------------ELEDTWRKQISA 556
            .            ||     ..|:.|                           :||..::::||
pombe   280 QGDTFNLARQKLLEKSAILSAAEVWKSEHESVLNRGNLQVPKNVSSLFYSWYVQLEQLFKEEIS- 343

  Fly   557 AIVRDLNTLRAQVRFKPHGFMNYYTYLKTLDASHFADILIKELYKLA------EGSETFSPTVGQ 615
             ::.|| .|...:. |.:..:.|..:||.|.:...|.:.|.|:.:|:      .|:.     |..
pombe   344 -LIDDL-ALNESLD-KKNDRLIYGPFLKLLSSKKLAALTIMEVAQLSTNPRYDRGAR-----VTT 400

  Fly   616 LYKELGQKVQQRY---QIEQKKHNGTL---EKIGEIYSSYCELWGSGMSQDNTRQAWQRL-VYEQ 673
            |...||:..::.:   ||::::.|.:.   :::.|:::          .....|||.:.| :...
pombe   401 LLGGLGRSFEREFLSEQIQRQEKNKSYKDKKRLKELFN----------DPRKFRQAVKNLRLSNT 455

  Fly   674 RES---GPSMDLLEVPWPSNVLTGVGRFLYNILMRDIKIDAHIMRQKSKSKAAAQPQNLLPAFYT 735
            |::   .||:|    .|||.::..||.....:|:...||:   :..|..|......|.:....:|
pombe   456 RDNIVLNPSVD----SWPSAIVMKVGSVALCLLLSVAKIE---VTAKDLSTGGILKQEVAAFVHT 513

  Fly   736 LFRNQGRFVKEEVKPHPVLSRLFRASRQQTLTFDSNLVPMLCPPQPWSTPHNGGYLLNKTELIRL 800
            ...:.||.| ..:.||....:|.....::. .....|:|||..|:||::..:|||..::..|:||
pombe   514 YQYSNGRKV-GMIVPHVEFYKLLSRDIEKP-HLHPQLLPMLVTPKPWTSWIDGGYYYSRQPLVRL 576

  Fly   801 PHQAIQQWDRIM-ASNPQHLYPALDSLNQLASVPWRVNTQLLDVIIKVFQNGGDVKLDVP--QPP 862
             ..|::|.|.:| ||....|.....:::.|..|.||:|.:|.:|:|::: |.|:..|.:|  :..
pombe   577 -KGALEQVDYLMKASENGQLDELFKAVSSLGKVSWRINQRLFNVLIRIW-NSGEKFLSIPPREVK 639

  Fly   863 SSLPPLPTLPAKDAEGNATSSEDRAKQFRDKLGH-RRKQ-----AEMYSLWCDALYRLSLAQHYR 921
            ..:||.|.        |:.:..|:.      :.| |||:     ...:|..||..|:|.:|:.:.
pombe   640 CDMPPYPK--------NSINPRDKV------IWHTRRKELAALKTGAHSQRCDFNYKLEIARAFL 690

  Fly   922 DKVFWLPHNMDFRGRVYPVPPHLNHLGSDLARSMLIFDQAQPLGVDGLSWLKLHCINLTGLKKRD 986
            ::.|:.||::|||||.||:..||:|:.:|:.|.:|.|...:|||..||:|||:|..||.|:.|:|
pombe   691 NEKFYFPHSLDFRGRAYPLSSHLHHVSNDVCRGLLEFSTGKPLGPKGLNWLKVHLANLFGISKKD 755

  Fly   987 SVRERLLYAEEIMPDILDSADNPLTGRMWWSKSDEPWQTLACCMEIANVHRCPDPAAYLSRFPIH 1051
             ...|..:.::.|.::.||||.||.|..||||:|:|:|.||.|.|||...|..|..:|:|..||.
pombe   756 -FATRQAFVDDNMQEVFDSADRPLDGNKWWSKADDPFQALAACFEIAEAVRSGDHESYISHIPIQ 819

  Fly  1052 QDGSCNGLQHYAALGRDEAGARSVNLAPSAIPQDVYSAVAALVEKSRKADAQNGLHVAEALAGFV 1116
            |||:|||||||||||.|..||:.|||.||..|.|||.|||.:|....|.||:.|..:|..|...|
pombe   820 QDGTCNGLQHYAALGGDIEGAKQVNLWPSDHPSDVYEAVAEIVRGFLKKDAEAGDEMANFLKDKV 884

  Fly  1117 RRKVIKQTVMTTVYGVTRYGARLQIARQLKDIDDFPKDWVWPASTYLTTKTFESLREMFTSTREI 1181
            .|.|:|.||||.|||||..|||.||:.:|::||...|..|...:.|||.|.||:||.:||...||
pombe   885 TRSVVKPTVMTNVYGVTYVGARKQISEKLENIDGMEKLKVADYANYLTKKVFEALRSLFTQAHEI 949

  Fly  1182 QDWFTECARLIS----------GVCSQ--NVEWVTPLGLPVVQPYNRQEMKHSPRSGFKVSANMP 1234
            |||.:.|..||:          |:..:  .|.|.|.|.||:||||...:.:       ::..|:.
pombe   950 QDWLSACCNLITHSLPADYIKEGIKDELTPVVWTTLLNLPIVQPYRNYKSR-------QIRTNLQ 1007

  Fly  1235 MDLYE--------RPNILKQKNAFPPNFIHSLDSSHMMLTSLHCERQGITFVSVHDCFWTHANTV 1291
            ....|        :|:  ||..|||||||||||::||.:|.|.|..|.|.|.:|||.:||||..|
pombe  1008 TVFIEERDRTATVQPH--KQATAFPPNFIHSLDATHMFMTCLKCSEQNINFAAVHDSYWTHACDV 1070

  Fly  1292 PELNRMCREQFVALHSQPILEQLSEFMRHTYS----FKDSDFINDGSVEDLSKRQLNRI-----L 1347
            .::|.:.||.||.|||..|:|:|.:.....|.    .|.:...||   |||..:..|:.     .
pombe  1071 DQMNSLLREAFVLLHSNNIMERLKQEFEERYKGFLVSKKAIKAND---EDLKAKFGNKSYIPLEF 1132

  Fly  1348 KQMPQKGDFDLRNVLDSVYFFS 1369
            ..:|.:|..||:.||:|.||||
pombe  1133 PPLPARGALDLKKVLESKYFFS 1154

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mtRNApol	NP_001259853.1	RPO41	286..1369	CDD:227439	367/1209 (30%)
		PPR repeat	314..342	CDD:276811	4/37 (11%)
		PPR repeat	355..384	CDD:276811	6/31 (19%)
		PPR	358..386	CDD:279828	6/30 (20%)
		RPOL_N	513..829	CDD:291372	84/376 (22%)
		RNA_pol	962..1368	CDD:279308	189/434 (44%)
rpo41	NP_594459.2	RPO41	35..1154	CDD:227439	373/1243 (30%)
		RPOL_N	288..606	CDD:291372	75/346 (22%)
		MreC	<411..>478	CDD:302802	16/80 (20%)
		RNA_pol	731..1153	CDD:279308	189/434 (44%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	336	1.000	Domainoid score	I196
eggNOG	1	0.900	-	-		E1_COG5108
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	448	1.000	Inparanoid score	I299
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003398
OrthoInspector	1	1.000	-	-		oto102164
orthoMCL	1	0.900	-	-		OOG6_101824
Panther	1	1.100	-	-	LDO	PTHR10102
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1209
SonicParanoid	1	1.000	-	-		X2294
TreeFam	1	0.960	-	-
	12	11.850