DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pkg21D and Prkg2

DIOPT Version :9

Sequence 1:	NP_477213.1	Gene:	Pkg21D / 33253	FlyBaseID:	FBgn0000442	Length:	768	Species:	Drosophila melanogaster
Sequence 2:	NP_037144.1	Gene:	Prkg2 / 25523	RGDID:	3401	Length:	762	Species:	Rattus norvegicus

Alignment Length:	783	Identity:	345/783 - (44%)
Similarity:	491/783 - (62%)	Gaps:	71/783 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly    17 NLTKDVQALREMVRSRESELVKLHREIHKLKSVLQQTTNNLNVTRNEKAKKKLYSLPEQCGEQES 81
            ||:.  :|||       |::.:|.||:.:..:.||:...:|...|.:.||:.: ::.|...|.:|
  Rat    20 NLSN--EALR-------SKVAELEREVKRKDAELQEREYHLKELREQLAKQTV-AIAELTEELQS 74

  Fly    82 RNQNPHLCSSCGMVLPTSPEFALEALSLGPLSPLASTSSASPSGRTSADEV------RPKAMPAA 140
            :                       .:.|..|..:......||. :.|.|:|      :...:.:.
  Rat    75 K-----------------------CIQLNKLQDVIHVQGGSPL-QASPDKVPLDVHRKTSGLVSL 115

  Fly   141 IKKQGVSAESCVQSMQQSYSIPIP--------KYEKDFSDKQQIKDAIMDNDFLKNIDASQVREL 197
            ..::|..|....:...::|.:..|        :..||.|:|:.|.||:..|.|||.:|..|::::
  Rat   116 HSRRGAKAGVSAEPTSRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDM 180

  Fly   198 VDSMYSKSIAAGEFVIREGEVGAHLYVSAAGEFAVMQQGKVLDKMGAGKAFGELAILYNCTRTAS 262
            |:.||.::...|.:::::||.|.|::|.|.|...|.|..|:|..:.....|||||||||||||||
  Rat   181 VECMYGRNYQQGSYIVKQGEPGNHIFVLAEGRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTAS 245

  Fly   263 IRVLSEAARVWVLDRRVFQQIMMCTGLQRIENSVNFLRSVPLLMNLSEELLAKIADVLELEFYAA 327
            ::.::. .:.|.|||.|||.||..|...|.|...||||||.||.||.|:.|.||.|.||:|:|..
  Rat   246 VKAITN-VKTWALDREVFQNIMRRTAQARDEEYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDK 309

  Fly   328 GTYIIRQGTAGDSFFLISQGNVRVTQKLTPTSPEETELRTLSRGDYFGEQALINEDKRTANIIAL 392
            |.||||:|..|.:||::::|.|:|||. |....:...::||.:|:||||:|||::|.|:|||||.
  Rat   310 GDYIIREGEEGSTFFILAKGKVKVTQS-TEGHDQPQLIKTLQKGEYFGEKALISDDVRSANIIAE 373

  Fly   393 SPGVECLTLDRDSFKRLIGDLCELKEKDYG--------DESR---------KLAMKQAQESCRDE 440
            ...|.||.:||::|.:.:|...||::...|        ||.|         ||:...:.|..  :
  Rat   374 ENDVACLVIDRETFNQTVGTFDELQKYLEGYVATLNRDDEKRHAKRSMSSWKLSKALSLEMI--Q 436

  Fly   441 PKEQLQQEFPDLKLTDLEVVSTLGIGGFGRVELVKAHHQDRVDIFALKCLKKRHIVDTKQEEHIF 505
            .||::.:........:||:::|||:||||||||||..:::..  ||:||::|:|||||||:||::
  Rat   437 LKEKVARFSSTSPFQNLEIIATLGVGGFGRVELVKVKNENIA--FAMKCIRKKHIVDTKQQEHVY 499

  Fly   506 SERHIMLSSRSPFICRLYRTFRDEKYVYMLLEACMGGEIWTMLRDRGSFEDNAAQFIIGCVLQAF 570
            ||:.|:....||||.:|||||:|.||||||||||:|||:|::|||||||::..::|.:.||.:||
  Rat   500 SEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAF 564

  Fly   571 EYLHARGIIYRDLKPENLMLDERGYVKIVDFGFAKQIGTSSKTWTFCGTPEYVAPEIILNKGHDR 635
            :|||..||||||||||||:||..||:|:|||||||:||:..|||||||||||||||:|||||||.
  Rat   565 DYLHRLGIIYRDLKPENLILDADGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDF 629

  Fly   636 AVDYWALGILIHELLNGTPPFSAPDPMQTYNLILKGIDMIAFPKHISRWAVQLIKRLCRDVPSER 700
            :||:|:||||::|||.|.||||..|.|.|||||||||:.:.||:.|:|....||:||||..|:||
  Rat   630 SVDFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTER 694

  Fly   701 LGYQTGGIQDIKKHKWFLGFDWDGLASQLLIPPFVRPIAHPTDVRYFDRFPCDLNEPPDELSGWD 765
            ||....||.|||||:|..||:|:||.::.|..|..|.::.|.|..|||::|.:...||||:||||
  Rat   695 LGNLKNGINDIKKHRWLNGFNWEGLKARSLPSPLRRELSGPIDHSYFDKYPPEKGVPPDEMSGWD 759

  Fly   766 ADF 768
            .||
  Rat   760 KDF 762

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkg21D	NP_477213.1	Crp	183..386	CDD:223736	93/202 (46%)
		CAP_ED	185..285	CDD:237999	43/99 (43%)
		CAP_ED	304..419	CDD:237999	54/114 (47%)
		Pkinase	457..717	CDD:278497	167/259 (64%)
		STKc_cGK	463..724	CDD:270724	168/260 (65%)
		S_TK_X	719..>760	CDD:214529	16/40 (40%)
Prkg2	NP_037144.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..25		2/6 (33%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	112..138		3/25 (12%)
		Crp	162..>285	CDD:223736	54/123 (44%)
		cGMP-binding, high affinity, cAMP-binding, moderate affinity. /evidence=ECO:0000250\|UniProtKB:Q13237	168..283		50/115 (43%)
		CAP_ED	168..273	CDD:237999	45/105 (43%)
		cGMP-binding, high affinity, cAMP-binding, low affinity. /evidence=ECO:0000250\|UniProtKB:Q13237	286..416		57/130 (44%)
		CAP_ED	286..401	CDD:237999	54/115 (47%)
		PTZ00263	417..752	CDD:140289	187/338 (55%)
		STKc_cGK	459..718	CDD:270724	168/260 (65%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	740..762		12/21 (57%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	78	1.000	Domainoid score	I8628
eggNOG	1	0.900	-	-		E1_COG0664
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H4556
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG57714
OrthoDB	1	1.010	-	-		D123183at33208
OrthoFinder	1	1.000	-	-		FOG0001832
OrthoInspector	1	1.000	-	-		oto97849
orthoMCL	1	0.900	-	-		OOG6_100630
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X815
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	11	10.730