DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ebi and SIF2

DIOPT Version :9

Sequence 1:	NP_477329.1	Gene:	ebi / 33212	FlyBaseID:	FBgn0263933	Length:	700	Species:	Drosophila melanogaster
Sequence 2:	NP_009661.1	Gene:	SIF2 / 852399	SGDID:	S000000307	Length:	535	Species:	Saccharomyces cerevisiae

Alignment Length:	752	Identity:	151/752 - (20%)
Similarity:	249/752 - (33%)	Gaps:	274/752 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSFSSDEVNFLVYRYLQESGFLHSAYVFGIESHISQSNIN-GALVPPAALLTILQKGLLYTEVEW 64
            ||.:|:|:|:|::||.||.|...||.....|:.:.:.:.. ...:|...|:.::|:|:||||.|.
Yeast     1 MSITSEELNYLIWRYCQEMGHEVSALALQDETRVLEFDEKYKEHIPLGTLVNLVQRGILYTESEL 65

  Fly    65 SVGEDGEVA-----RPIEGLSLIDAVMPEVKPLKPIVKTEPGKPGAVDSSAPAGGNQNNNAKPEI 124
            .|...|:::     ...|..:|:.|:.                   :|.
Yeast    66 MVDSKGDISALNEHHLSEDFNLVQALQ-------------------IDK---------------- 95

  Fly   125 KIEPGTGVAGSAGGNKIAGSTTGTSTPTDQSASEVDSSGNAANNAGGTYAGNNGAGGNQASTGGS 189
                                         :...|:.|.|.                    .|..:
Yeast    96 -----------------------------EKFPEISSEGR--------------------FTLET 111

  Fly   190 NSTSTPAGGDLAAPGASQKKSQNSNEAGSSSSGNAGNANATSTDDAASSTSTNGNSSTSSSVEQP 254
            ||.|..||.|    |||..:.:                  |..||.        ||..||.
Yeast   112 NSESNKAGED----GASTVERE------------------TQEDDT--------NSIDSSD---- 142

  Fly   255 TSGLTPAGGTVSTSNPDAAASGGASTATGSKAPSGAVTIRVGAQGNNVQSGSSNAQSSAPSGTIS 319

Yeast   143 ----------------------------------------------------------------- 142

  Fly   320 SSTSGGAGTPAALVPMDIDENIEIPESKARVLRGHESEVFICAWNP-SRDLLASGSGDSTARIWD 383
                            |:|..::|.:...::     ..:....||| ...:||.|..:|.||:..
Yeast   143 ----------------DLDGFVKILKEIVKL-----DNIVSSTWNPLDESILAYGEKNSVARLAR 186

  Fly   384 MSDANTNSNQ-------LVLRHCIQKGGAEVPSNKDVTSLDWNCDGSLLATGSYDGYARIWKTDG 441
            :.:.:....:       ..|||......:...:...||.|.|:.||:.:.||..:|..|:|...|
Yeast   187 IVETDQEGKKYWKLTIIAELRHPFALSASSGKTTNQVTCLAWSHDGNSIVTGVENGELRLWNKTG 251

  Fly   442 RLASTLGQHKGPIFALKWNKCGNYILSAGVDKTTIIWDASTGQCTQQFAF------------HSA 494
            .|.:.|..|:.||.::||||.|.:|:|..|:..||:|:..:|...|.|..            ||.
Yeast   252 ALLNVLNFHRAPIVSVKWNKDGTHIISMDVENVTILWNVISGTVMQHFELKETGGSSINAENHSG 316

  Fly   495 P---ALDVDWQTNQAFASCSTDQRIHVCRLGVNEPIKTFKGHTNEVNAIKWCPQGQLLASCSDDM 556
            .   .:||:|..:..|........|.|.::....|.....||...::.:::....:||.|.|||.
Yeast   317 DGSLGVDVEWVDDDKFVIPGPKGAIFVYQITEKTPTGKLIGHHGPISVLEFNDTNKLLLSASDDG 381

  Fly   557 TLKIW---SMNRDRCCHDLQAHSKEIYTIKWSPTGPGTNNPNTNLILASASFDSTVRLWDVERGS 618
            ||:||   :.|...|.:   .||:.|.:..|    .|.:.      :.|.|.|.:||||.:::.:
Yeast   382 TLRIWHGGNGNSQNCFY---GHSQSIVSASW----VGDDK------VISCSMDGSVRLWSLKQNT 433

  Fly   619 CIHTLTKHTEPVYSVAFSPDGKHLASGSFDKCVHI-------------WSTQTG-------QLVH 663
            .:........|:::...|.||:..|....|..|::             :..:.|       .|..
Yeast   434 LLALSIVDGVPIFAGRISQDGQKYAVAFMDGQVNVYDLKKLNSKSRSLYGNRDGILNPLPIPLYA 498

  Fly   664 SYKGTGG---IFEVCWNSKGTKVGA--SASDGSVFVL 695
            ||:.:..   ||::.||..|.|:..  |..:|||..:
Yeast   499 SYQSSQDNDYIFDLSWNCAGNKISVAYSLQEGSVVAI 535

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ebi	NP_477329.1	LisH	6..32	CDD:285685	10/25 (40%)
		WD40	349..655	CDD:238121	85/344 (25%)
		WD40 repeat	358..402	CDD:293791	12/51 (24%)
		WD40 repeat	414..449	CDD:293791	13/34 (38%)
		WD40 repeat	454..490	CDD:293791	14/35 (40%)
		eIF2A	457..665	CDD:285825	58/245 (24%)
		WD40 repeat	497..531	CDD:293791	7/33 (21%)
		WD40 repeat	537..573	CDD:293791	12/38 (32%)
		WD40 repeat	579..624	CDD:293791	10/44 (23%)
		WD40 repeat	630..654	CDD:293791	6/36 (17%)
SIF2	NP_009661.1	LisH	7..31	CDD:369916	10/23 (43%)
		WD40	220..516	CDD:421866	80/308 (26%)
		WD40 repeat	224..259	CDD:293791	13/34 (38%)
		WD40 repeat	264..300	CDD:293791	14/35 (40%)
		WD40 repeat	322..356	CDD:293791	7/33 (21%)
		WD40 repeat	362..398	CDD:293791	12/35 (34%)
		WD40 repeat	445..482	CDD:293791	6/36 (17%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157342020
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0273
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	175	1.000	Inparanoid score	I984
Isobase	1	0.950	-	0.993157	Normalized mean entropy	S2019
OMA	1	1.010	-	-		QHG53955
OrthoFinder	1	1.000	-	-		FOG0002050
OrthoInspector	1	1.000	-	-		oto99347
orthoMCL	1	0.900	-	-		OOG6_102387
Panther	1	1.100	-	-	LDO	PTHR22846
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R560
SonicParanoid	1	1.000	-	-		X1519
TreeFam	0	0.000	Not matched by this tool.
	12	11.780