DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ebi and hif2

DIOPT Version :9

Sequence 1:	NP_477329.1	Gene:	ebi / 33212	FlyBaseID:	FBgn0263933	Length:	700	Species:	Drosophila melanogaster
Sequence 2:	NP_587735.1	Gene:	hif2 / 2538701	PomBaseID:	SPCC1235.09	Length:	564	Species:	Schizosaccharomyces pombe

Alignment Length:	676	Identity:	142/676 - (21%)
Similarity:	245/676 - (36%)	Gaps:	188/676 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 SDEVNFLVYRYLQESGFLHSAYVFGIESHISQSNIN---GALVPPAALLTILQKGLLYTEVE--- 63
            :::||::::|||:|.|:.|:.:.|..|:.|  .|::   |:.....||:.||||||.|.|:|
pombe     3 TNQVNYIIWRYLKECGYSHTKFAFERETGI--QNLDKQWGSTCQVGALVEILQKGLQYVELEKHY 65

  Fly    64 ---WSVGEDGEVARPIEGLSLID---AVMPEVKPLKPIVKTEPGKPGAVDSSAPAGGNQNNNAKP 122
               .|..|:.. ...|:|.||::   ..:|....:..|.:|...|..:.:...    ..||:...
pombe    66 VDNHSSNEEAS-KTSIDGESLVNENPCKLPFYLTVPHICETTLTKADSTNGFC----EHNNSNDH 125

  Fly   123 EIKIEPGTGVAGSAGGNKIAGSTTGTSTPTDQSASEVDSSGNAANNAGGTYAGNNGAGGNQASTG 187
            ::||....|          :||.:....|......:.|.....|:.
pombe   126 QLKILQDKG----------SGSPSSPVMPFKDKIEKRDIDITMADE------------------- 161

  Fly   188 GSNSTSTPAGGDLAAPGASQKKSQNSNEAGSSSSGNAGNANATSTDDAASSTSTNGNSSTSSSVE 252
             ||....|     |.|.|....|.                 .|...:....|.|.|....|...:
pombe   162 -SNVEKDP-----ARPIAVYNSSP-----------------VTEITEIKQVTFTGGEDIKSDFFK 203

  Fly   253 QPTSGLTPAGGTVSTSNPDAAASGGASTATGSKAPSGAVTIRVGAQGNNVQSGSSNAQSSAPSGT 317
                       .:.|.:|...|...
pombe   204 -----------VIPTKHPVTCADWR---------------------------------------- 217

  Fly   318 ISSSTSGGAGTPAALVPMDIDENIEIPESKARVLRGHESEVFICAWNPSRDLLASGSGDSTARIW 382
                            |: :.||..:.|....:.....:.|.||.
pombe   218 ----------------PL-LQENYHVYEFSIGMTNATLASVSICE-------------------- 245

  Fly   383 DMSDANTNSNQLVLRHCIQKGGAEVPSNKDVTSLDWNCDGSLLATGSYDGYARIWKTDGRLASTL 447
            :.:|....::     :|:|...    .|:|:|.:.||..||.||...:.|...|:.:.|....:.
pombe   246 EQNDFKAKTD-----YCLQSSF----DNQDITGVAWNNSGSFLAYAFFSGVIEIYDSHGSQILSF 301

  Fly   448 GQHKGPIFALKWNKCGNYILSAGVDKTTIIWDASTGQCTQ-QFAFH--SAPALDVDWQTNQAFAS 509
            ..:|||:.:|||:....|:.:...|.|..::|    |..| |::..  ::..||::|.:...|.:
pombe   302 HNNKGPVLSLKWSGTDTYLAAGSADGTITLFD----QLKQTQYSIDTLASSVLDIEWISFDEFVT 362

  Fly   510 CSTDQRIHVCRLGVNEPIKTFK-GHTNEVNAIKWCPQGQLLASCSDDMTLKIWS---MNRDRCCH 570
            ...:..:.|.::....|:.|.. .|.|.:.|:::..:..||.:.|.|.|:|:||   .....|.|
pombe   363 SDVEGSLRVYKVDGKAPVSTVSHAHDNSIVALRYNLRISLLLTASSDTTVKLWSRGDAGAFECLH 427

  Fly   571 DLQAHSKEIYTIKWSPTGPGTNNPNTNLILASASFDSTVRLWDVERGSCIHTLTKHTEPVYSVAF 635
             :.:.|..:..|.|: ...||.      |||.|| :|.|.:::......:....:||.||.:::|
pombe   428 -VFSFSSPVNCIDWN-LREGTP------ILAVAS-NSIVSMYNAISLQQLAVFMRHTAPVSALSF 483

  Fly   636 SPDGKHLASGSFDKCVHIWSTQTGQL 661
            |.:|::||:|.....|.|||.:|.:|
pombe   484 SHNGRYLATGDTSGGVCIWSCKTAKL 509

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ebi	NP_477329.1	LisH	6..32	CDD:285685	9/25 (36%)
		WD40	349..655	CDD:238121	75/312 (24%)
		WD40 repeat	358..402	CDD:293791	5/43 (12%)
		WD40 repeat	414..449	CDD:293791	10/34 (29%)
		WD40 repeat	454..490	CDD:293791	10/36 (28%)
		eIF2A	457..665	CDD:285825	58/212 (27%)
		WD40 repeat	497..531	CDD:293791	7/33 (21%)
		WD40 repeat	537..573	CDD:293791	11/38 (29%)
		WD40 repeat	579..624	CDD:293791	11/44 (25%)
		WD40 repeat	630..654	CDD:293791	8/23 (35%)
hif2	NP_587735.1	LisH	4..30	CDD:285685	9/25 (36%)
		WD40	<263..524	CDD:225201	73/260 (28%)
		WD40	267..523	CDD:295369	71/256 (28%)
		WD40 repeat	267..301	CDD:293791	10/33 (30%)
		WD40 repeat	309..347	CDD:293791	10/41 (24%)
		WD40 repeat	349..384	CDD:293791	7/34 (21%)
		WD40 repeat	392..430	CDD:293791	11/38 (29%)
		WD40 repeat	435..472	CDD:293791	11/44 (25%)
		WD40 repeat	478..515	CDD:293791	13/32 (41%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0273
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	152	1.000	Inparanoid score	I1296
OMA	1	1.010	-	-		QHG53955
OrthoFinder	1	1.000	-	-		FOG0002050
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_102387
Panther	1	1.100	-	-	LDO	PTHR22846
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R560
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	8	7.900