Protein Alignment Polr1B and RPA135

DIOPT Version :10

• Sequence 1: NP_476708.1
• Gene: Polr1B / 33210
• FlyBaseID: FBgn0003278
• Length: 1129
• Species: Drosophila melanogaster
• Sequence 2: NP_015335.1
• Gene: RPA135 / 856119
• SGDID: S000006214
• Length: 1203
• Species: Saccharomyces cerevisiae
• Alignment Length: 1176
• Identity: 492/1176 - (41%)
• Similarity: 705/1176 - (59%)
• Gaps: 106/1176 - (9%)

  Fly    38 PHVDSFDEMLTVGLD----------------------NSAKHMIPNHWLSPAGEKISMKVESIWI 80
            ||:.||: .||.|.|                      ||...:..:.:|   |.|:|:.||.:.|
Yeast    44 PHIGSFN-ALTEGPDGGLLNLGVKDIGEKVIFDGKPLNSEDEISNSGYL---GNKLSVSVEQVSI 104

  Fly    81 AKPKVPQDVIDVRTREIYPTDSRQLHVSYSGMCSVRLGWSVNGVQKTPINM-DLGEVPIMLRSKA 144
            |||.....|.....|::||::|||...||.|...::|.||||..::....: |.|.:|:||:|..
Yeast   105 AKPMSNDGVSSAVERKVYPSESRQRLTSYRGKLLLKLKWSVNNGEENLFEVRDCGGLPVMLQSNR 169

  Fly   145 CNLGQATPEEMVKHGEHDSEWGGIFVIRGNEKIVRMLIMTRRNHPICVKRSSWKDRGQNFSDLGM 209
            |:|.:.:|.|:|:|.|...|.||.|::.|.||::||||:.|||||:.:.|.|:.:||.::|..|:
Yeast   170 CHLNKMSPYELVQHKEESDEIGGYFIVNGIEKLIRMLIVQRRNHPMAIIRPSFANRGASYSHYGI 234

  Fly   210 LVQTVREDESSLSNVVHYLNNGTAKFMFSHVKRLSYVPVCLILKCLMDYTDEEIYNRLVQGYESD 274
            .:::||.|::|.:||:||||:|...|.||..|....|||.:|||.|...:|.||::.::.....|
Yeast   235 QIRSVRPDQTSQTNVLHYLNDGQVTFRFSWRKNEYLVPVVMILKALCHTSDREIFDGIIGNDVKD 299

  Fly   275 QYYVSCVQAMLREVQNENVYTHAQCKS----FIGNLFRARFPEVPEWQPDDDVTDFILRERVMIH 335
            .:....::.:||..:..  |.|.|.::    ::|:.||..|...|: |.|.:|...:|...|::|
Yeast   300 SFLTDRLELLLRGFKKR--YPHLQNRTQVLQYLGDKFRVVFQASPD-QSDLEVGQEVLDRIVLVH 361

  Fly   336 L--DTYEDKFQLIVFMIQKLFQCAQGKYKVENVDSAMMQEVLLPGHLYQKYLSERVESW----VS 394
            |  |..:|||::::|||:||:....|:...:|.|:...|||||.|.||...|.|:::.:    ::
Yeast   362 LGKDGSQDKFRMLLFMIRKLYSLVAGECSPDNPDATQHQEVLLGGFLYGMILKEKIDEYLQNIIA 426

  Fly   395 QVRRCLQK--KLTSPDALVTSAVMTQCMRQAGGVGRAIESFLATGNIASRTGLGLMQNSGLVIMA 457
            |||..:.:  .:...|....|.|:   ||....:|..::.||:|||:.|::||.|.|.||..::|
Yeast   427 QVRMDINRGMAINFKDKRYMSRVL---MRVNENIGSKMQYFLSTGNLVSQSGLDLQQVSGYTVVA 488

  Fly   458 ENINRMRYMSHFRAIHRGSYFTTMRTTEARQLLPDAWGFICPVHTPDGTPCGLLNHLTLTCEISM 522
            |.||..|::||||.:||||:|..::||..|:|||::|||:||||||||:|||||||....|.||.
Yeast   489 EKINFYRFISHFRMVHRGSFFAQLKTTTVRKLLPESWGFLCPVHTPDGSPCGLLNHFAHKCRIST 553

  Fly   523 RPDPKLVKAIPKHLIDMGMMPLSNRRYLGEKLYVVFLDGKHLGHIHQSEAEKIVDELRYGKIFGT 587
            :...  |..||..|..:|:.|.|:....|..|..|.:|||.:|.:...:.:.|.|.|||.|:.|.
Yeast   554 QQSD--VSRIPSILYSLGVAPASHTFAAGPSLCCVQIDGKIIGWVSHEQGKIIADTLRYWKVEGK 616

  Fly   588 LPQM---MEIGFIPFKKNGQFPGLYIATGPARMMRPVWNLKWKRVEYIGTLEQLYMEIAIDAKEM 649
            .|.:   :|||::|....||:||||:..|.:||:|||..|...:.:.:|..||:||.||:..:|:
Yeast   617 TPGLPIDLEIGYVPPSTRGQYPGLYLFGGHSRMLRPVRYLPLDKEDIVGPFEQVYMNIAVTPQEI 681

  Fly   650 YPDFTTHLELAKTHFMSNLANLIPMPDYNQSPRNMYQCQMGKQTMGTPCLNWPKQAANKLYRLQT 714
            ..:..||:|...|:.:|.||||.|..|:||||||||||||||||||||.:....::.||||||||
Yeast   682 QNNVHTHVEFTPTNILSILANLTPFSDFNQSPRNMYQCQMGKQTMGTPGVALCHRSDNKLYRLQT 746

  Fly   715 PGTPLFRPVHYDNIQLDDFAMGTNAIVAVISYTGYDMEDAMIINKAAYERGFAYGSIYKTKFLTL 779
            ..||:.:...||:..:|:|..|.||:|||||||||||:|||||||:|.||||.||::|||:.:.|
Yeast   747 GQTPIVKANLYDDYGMDNFPNGFNAVVAVISYTGYDMDDAMIINKSADERGFGYGTMYKTEKVDL 811

  Fly   780 -------DKKSSYFA-RHPHMP-ELIKHLDTDGLPHPGSKLSYGSPLYCYFDGEVATYKVVKMDE 835
                   |..:.:|. .:...| |.::.||.||||:.|:.:..|.|:..|||..:...|:.....
Yeast   812 ALNRNRGDPITQHFGFGNDEWPKEWLEKLDEDGLPYIGTYVEEGDPICAYFDDTLNKTKIKTYHS 876

  Fly   836 KEDCIVESIRQLGSFDLSPK----KMVAITLRVPRPATIGDKFASRAGQKGICSQKYPAEDLPFT 896
            .|...:|.:..:|  |.|.|    :.|:|..|:.|...|||||:||.||||:||:|:|..|:||:
Yeast   877 SEPAYIEEVNLIG--DESNKFQELQTVSIKYRIRRTPQIGDKFSSRHGQKGVCSRKWPTIDMPFS 939

  Fly   897 ESGLIPDIVFNPHGFPSRMTIAMMIETMAGKGAAIHGNVYDATPFRFSEENTAIDYFGKMLEAGG 961
            |:|:.|||:.|||.|||||||.|.:|::|||..|:||...|:||:.|:|::|..||||:.|...|
Yeast   940 ETGIQPDIIINPHAFPSRMTIGMFVESLAGKAGALHGIAQDSTPWIFNEDDTPADYFGEQLAKAG 1004

  Fly   962 YNYYGTERLYSGVDGREMTADIFFGVVHYQRLRHMVFDKWQVRSTGAVEARTHQPIKGRKRGGGV 1026
            |||:|.|.:|||..|.|:.|||:.|||:||||||||.||:||||||.|.:.|.||:|||||.||:
Yeast  1005 YNYHGNEPMYSGATGEELRADIYVGVVYYQRLRHMVNDKFQVRSTGPVNSLTMQPVKGRKRHGGI 1069

  Fly  1027 RFGEMERDALISHGAAFLLQDRLFHNSDKTHTLVCHKCGSILAPLQRIVKRNETGGLSSQPDTCR 1091
            |.|||||||||.||.:|||||||.::||.|...||.:|||||...|.:.:   .|.:|:.  .||
Yeast  1070 RVGEMERDALIGHGTSFLLQDRLLNSSDYTQASVCRECGSILTTQQSVPR---IGSISTV--CCR 1129

  Fly  1092 LCG---------------------DNSSV---------------SMIEIPFSFKYLVTELSSVNI 1120
            .|.                     |:|.:               :.:.|||..|||.:|||::.|
Yeast  1130 RCSMRFEDAKKLLTKSEDGEKIFIDDSQIWEDGQGNKFVGGNETTTVAIPFVLKYLDSELSAMGI 1194

  Fly  1121 NARFKL 1126
            ..|:.:
Yeast  1195 RLRYNV 1200

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr1B	NP_476708.1	RNA_pol_B_RPB2	35..1125	CDD:238353	492/1173 (42%)
		RNA_pol_Rpa2_4	564..619	CDD:462028	22/57 (39%)
RPA135	NP_015335.1	RNA_pol_B_RPB2	42..1199	CDD:238353	492/1173 (42%)
		RNA_pol_Rpb2_2	210..403	CDD:398318	68/195 (35%)
		RNA_pol_Rpa2_4	593..651	CDD:462028	22/57 (39%)