DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpI135 and Polr1b

DIOPT Version :9

Sequence 1:	NP_476708.1	Gene:	RpI135 / 33210	FlyBaseID:	FBgn0003278	Length:	1129	Species:	Drosophila melanogaster
Sequence 2:	NP_113961.2	Gene:	Polr1b / 83582	RGDID:	620822	Length:	1135	Species:	Rattus norvegicus

Alignment Length:	1141	Identity:	523/1141 - (45%)
Similarity:	731/1141 - (64%)	Gaps:	57/1141 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly    19 PEFKQIPKKLSRHLANLGGPHVDSFDEMLTVGLDNSAKHMIPNHWLSPAGEKISMKVESIWIAKP 83
            |.:...|::....|.:|...|||||:..:..||.: |...||....:...|:||:.:....|:.|
  Rat    22 PSYAVPPEQQKAALQDLTRAHVDSFNYAVLEGLSH-AVQAIPPFEFAFKDERISLTIVDAAISPP 85

  Fly    84 KVPQDVIDVRTREIYPTDSRQLHVSYSGMCSVRLGWSVNGVQKTPINMDLGEVPIMLRSKACNLG 148
            .||:..| .:...|||.:.|....:|.|..:..:.|:||||.|..|...||.||||::||.|||.
  Rat    86 AVPKGTI-CKELNIYPAECRGRRSTYRGKLTADISWAVNGVPKGIIKQFLGYVPIMVKSKLCNLY 149

  Fly   149 QATPEEMVKHGEHDSEWGGIFVIRGNEKIVRMLIMTRRNHPICVKRSSWKDRGQNFSDLGMLVQT 213
            ...|:.:::|.|...|.||.|:|.|.||::|||||.|||.||.:.|..||.||..::..|:.:..
  Rat   150 NLPPQVLIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKSRGLGYTQFGVSIHC 214

  Fly   214 VREDESSLSNVVHYLNNGTAKFMFSHVKRLSYVPVCLILKCLMDYTDEEIYNRLVQGYESDQYYV 278
            |||:.|:::..:||:.|||....|.:.|.|.::|:...||.|:.::|.:|:..|::|.|.|.::.
  Rat   215 VREEHSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDSFFK 279

  Fly   279 SCVQAMLREVQNENVYTHAQCKSFIGNLFRARFPEVPEWQPDDDVTDFILRERVMIHLDTYEDKF 343
            :.|..|||.|..|..:|..|...::|..||.:. .:|:|.|:.:..:|:..:.:.|||.:..|||
  Rat   280 NSVSQMLRIVMEEGCHTQKQVLDYLGERFRVKL-SLPDWYPNAEAAEFLFNQCICIHLKSNTDKF 343

  Fly   344 QLIVFMIQKLFQCAQGKYKVENVDSAMMQEVLLPGHLYQKYLSERVESWVSQVRRCLQKKLTSPD 408
            .|:..|.:|||..|:|:...:|.||.:.||||.||.|:..:|.|::|:|:..::..|.|:....:
  Rat   344 YLLCLMTRKLFALARGECMEDNPDSLVNQEVLTPGQLFLMFLKEKMENWLLSIKIALDKRAQKTN 408

  Fly   409 ALVTSAVMTQCMRQAGGVGRAIESFLATGNIASRTGLGLMQNSGLVIMAENINRMRYMSHFRAIH 473
            ..:.:..:.:.......:.|..|..|||||:.|:||||.||:|||.::|:.:|.:||:||||.:|
  Rat   409 VSINNENLMKIFSMGTELTRPFEYLLATGNLRSKTGLGFMQDSGLCVVADKLNFIRYLSHFRCVH 473

  Fly   474 RGSYFTTMRTTEARQLLPDAWGFICPVHTPDGTPCGLLNHLTLTCEISMRPDPKLV--KAIPKHL 536
            ||:.|..||||..|:|||::|||:||||||||.|||||||||..||:.    .|.|  .:||..|
  Rat   474 RGADFAKMRTTTVRKLLPESWGFLCPVHTPDGAPCGLLNHLTAVCEVV----TKFVYTASIPALL 534

  Fly   537 IDMGMMPLSN---RRYLGEKLYVVFLDGKHLGHIHQSEAEKIVDELRYGKIF--GTLPQMMEIGF 596
            ..:|:.|:..   |.|  ...|.|.|||..:|.:.:..|.::.|.||..|:.  ..:|..||:..
  Rat   535 CGLGVTPVDAAPCRPY--SDCYPVLLDGVMVGWVDKELAPEVADTLRRFKVLRERRVPPWMEVAL 597

  Fly   597 IPFK-KNGQFPGLYIATGPARMMRPVWNLKWKRVEYIGTLEQLYMEIAIDAKEMYPDFTTHLELA 660
            ||.. |...:|||::.|.|.|::|||.||:..:.|.:||:|||:|.|||...|::...:||.||.
  Rat   598 IPMTGKPSLYPGLFLFTTPCRLVRPVQNLELGKEELVGTMEQLFMNIAIFEDEVFGGVSTHQELF 662

  Fly   661 KTHFMSNLANLIPMPDYNQSPRNMYQCQMGKQTMGTPCLNWPKQAANKLYRLQTPGTPLFRPVHY 725
            ....:|.:||.||..|:||||||||||||||||||.|.|.:..::.|||||||||.:||.||..|
  Rat   663 PHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPCMY 727

  Fly   726 DNIQLDDFAMGTNAIVAVISYTGYDMEDAMIINKAAYERGFAYGSIYKTKFLTLDKK------SS 784
            |:..:|::.:|||||||||||||||||||||:|||::|||||:||:||::|:.|.:|      |.
  Rat   728 DHYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKFKQGDDSL 792

  Fly   785 YFARHPHMPELIKHLDTDGLPHPGSKLSYGSPLYCYF-----DGEVATYKVVKMDEKEDCIVESI 844
            .|...|..|.:::.||.||||..|:||.:|.|.|.|.     :|.|..||     .||:|:|::|
  Rat   793 VFGVKPGDPRVMQKLDNDGLPFIGAKLEFGDPYYGYLNLNTGEGFVVYYK-----SKENCVVDNI 852

  Fly   845 RQLGSFDLSPK-KMVAITLRVPRPATIGDKFASRAGQKGICSQKYPAEDLPFTESGLIPDIVFNP 908
            :...:...|.| |.|.:|:||||..|||||||||.|||||.|:.:||||:||||||::|||:|||
  Rat   853 KVCSNDTGSGKFKCVCVTVRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMMPDILFNP 917

  Fly   909 HGFPSRMTIAMMIETMAGKGAAIHGNVYDATPFRFSEENTAIDYFGKMLEAGGYNYYGTERLYSG 973
            |||||||||.|:||:||||.||:||..:|||||.|||||:|::|||:||:|.|||:|||||||||
  Rat   918 HGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSG 982

  Fly   974 VDGREMTADIFFGVVHYQRLRHMVFDKWQVRSTGAVEARTHQPIKGRKRGGGVRFGEMERDALIS 1038
            :.|.|:.||||.|||:||||||||.||:|||:|||.:..|:|||.||...||:||||||||||::
  Rat   983 ISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPIGGRNVQGGIRFGEMERDALLA 1047

  Fly  1039 HGAAFLLQDRLFHNSDKTHTLVCHKCGSILAPLQRIVKRNETGGLSSQPDT----------CRLC 1093
            ||.:|||.||||:.||::...||.||||:|:||           |...|.:          |.:|
  Rat  1048 HGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPL-----------LEKPPPSWSAMRNRKYNCTVC 1101

  Fly  1094 GDNSSVSMIEIPFSFKYLVTELSSVNINARFKLNEI 1129
            |.:.|:..:.:|:.|:|.|.||:::||  :.||:.|
  Rat  1102 GRSDSIDTVSVPYVFRYFVAELAAMNI--KVKLDVI 1135

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpI135	NP_476708.1	RpoB	34..1128	CDD:223163	519/1123 (46%)
		RNA_pol_B_RPB2	35..1125	CDD:238353	517/1119 (46%)
		RNA_pol_Rpa2_4	564..614	CDD:284335	16/52 (31%)
Polr1b	NP_113961.2	RNA_pol_B_RPB2	38..1133	CDD:238353	518/1121 (46%)
		RNA_pol_Rpb2_2	186..375	CDD:398318	66/189 (35%)
		RNA_pol_Rpa2_4	563..616	CDD:399696	16/52 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166340759
Domainoid	1	1.000	446	1.000	Domainoid score	I506
eggNOG	1	0.900	-	-		E1_COG0085
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H7133
Inparanoid	1	1.050	921	1.000	Inparanoid score	I340
OMA	1	1.010	-	-		QHG53969
OrthoDB	1	1.010	-	-		D42570at2759
OrthoFinder	1	1.000	-	-		FOG0000346
OrthoInspector	1	1.000	-	-		oto97878
orthoMCL	1	0.900	-	-		OOG6_102249
Panther	1	1.100	-	-	LDO	PTHR20856
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X3081
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	15	14.810